Antimicrobial activity of Satureja hortensis L. essential oil against pathogenic microbial strains

Mihajilov‐Krstev, Tatjana; Radnović, Dragan; Kitić, Dušanka; Stojanović-Radić, Zorica; Zlаtković, Bojаn

doi:10.2298/abs1001159m

Cited by 56 publications

(43 citation statements)

References 32 publications

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“…As a result of this and negative consumer perceptions of artificial preservatives, attention is shifting towards alternatives that are obtained from plant extracts (Mihajilov-Krstev et al, 2010). Dobre et al (2011) reported that in the variety of techniques available for conservation, food industry is investigating other techniques to replace traditional methods of conservation due to high demand by consumers for tasty and natural food.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Essential Oils From Some African Spices Against Two Strains of Bacillus cereus Isolated From Vegetable Salad

Uzeh¹,

Oguntosin²

2013

JFR

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Chemical preservatives have been used to preserve our foods against spoilage and pathogenic microorganisms over the years. Consumers now frown at this hence the need to source for preservatives from natural sources. In this investigation fifteen samples of vegetable salad were collected from retail outlets in Lagos, Nigeria dilutions of which were plated on Brilliance Bacillus cereus agar. Isolates were identified based on morphological, biochemical characteristics and reactions to API 20E and API 50 CHB/E test kit. Two strains of Bacillus cereus; B. cereus 1 and B. cereus 2 were isolated. The organism was spread evenly on Mueller -Hinton agar and wells of 5mm in diameter were made on the inoculated agar with sterile cork borer. Different dilutions (1, 0.1, 0.01, 0.001 mg/ml) of essential oils extracted from three African spices; Aframomum melegueta, Xylopia aethiopica and Piper guineense by hydro distillation method were introduced into the wells in triplicate. After incubation diameter of zones of inhibition were measured. The essential oils of the spices inhibited growth of the two strains. A. melegueta produced the greatest zone of inhibition (14 to 24 mm) and with the lowest minimum inhibitory concentration (MIC) of 31.25 mg/ml, followed by X. aethiopica (10 to 18 mm) with MIC of 62.5 mg/ml and P. guineense (11 to 15 mm) and MIC of 125 and 250 mg/ml against B. cereus 1 and B. cereus 2 respectively. P. guineense was the least inhibitory. However, B. cereus 1 was more sensitive (inhibition zone of 12 to 24 mm) to the essential oils of the spices than B. cereus 2 (inhibition zone of 10 to 17 mm). The higher the concentration of the essential oils the greater the resultant zones of inhibition. The spices especially A. melegueta have proved very efficient in the inhibition of B. cereus a pathogen obtained from vegetable salad. The spices can therefore help in ensuring food safety.

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Section: Introductionmentioning

confidence: 99%

Efficacy of Essential Oils From Some African Spices Against Two Strains of Bacillus cereus Isolated From Vegetable Salad

Uzeh¹,

Oguntosin²

2013

JFR

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“…Each tube contained 9 mL of Muller Hilton was sterilize by autoclaving. After cooled, 1 mL of different concentrations (2,4,8,16, 32, 64, 128, 256 mg/mL) of each extract was added to each tube. The tubes were incubated for 24 hours at 37°C [14].…”

Section: Methodsmentioning

confidence: 99%

“…Owning to their aromatic and antiseptic properties, they are used as spices, natural food preservatives, as well as for aromatherapy and for different medical purposes in the perfume industry. Savory species produce antimicrobial secondary metabolites, essential oils, either as a part of their normal program of growth and development or in response to pathogens' attack or stress [2].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Effect of Aqueous and Ethanolic Extracts of Satureja Bachtiarica on Some Pathogenic Bacteria in Vitro

Heidari-Sureshjani

Tabatabaei-Yazdi

Alizadeh-Behbahani

et al. 2015

Zahedan J Res Med Sci

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Background: Essences and extracts of herbs, possess a variety level of biological activities, in addition the antimicrobial activities of a large number of these have been proved. With regard to the biologically active compounds and traditional use of the Satureja bachtiarica, it seems that this plant has significant antimicrobial effects. Objectives: The aim of this study is to evaluate the antimicrobial effect of aqueous and ethanolic extracts of S. bachtiarica against Enterobacter aerogenes, Bacillus subtilis and Listeria innocua of the important food pathogen. Materials and Methods:In this experimental study, antimicrobial effect of extracts was evaluated by two methods, Collins method and disk agar diffusion method on Enterobacter aerogenes ATTC 13048, Listeria innocua ATTC 33090 and Bacillus subtilis PTCC 1720. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determined using a dilution method. Statistical analysis was carried out by analysis of variance (ANOVA) technique. Results: All ethanolic extract concentrations had an inhibitory effect on the disk agar diffusion method. In Collins method ethanolic extract, prevented the growth of both strains on medium. The MBC of ethanolic extract of S. bachtiarica for B. subtilis and L. innocua was 16 and 32 mg/mL respectively, and for E. aerogenes was 64 mg/mL. But the MBC of aqueous extract of S. bachtiarica for B. subtilis and L. innocua was 32 and 64 mg/mL, respectively and for E. aerogenes was 128 mg/mL. Conclusions: The S. bachtiarica extract showed the more effective impact on the growth of B. subtilis PTCC 1720 and L. innocua ATTC 33090 than E. aerogenes ATTC 13048 (P < 0.05). The results indicated that ethanolic extract of S. bachtiarica had maximum effect on Gram positive bacteria B. subtilis PTCC 1720 and L. innocua ATTC 33090.

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“…Aromatic plants, known for both aromatic and antiseptic properties, are used in spices and natural food preservatives, perfume industry for aromatherapy, etc. (2,3). During the last decades, natural products with antimicrobial effect have been investigated for eliminating the use of synthetic antibiotics that cause the resistance of microorganisms and have side effects to human health (4).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial, antioxidant, cytotoxic and apoptotic activities of Satureja khuzestanica

Soltanzadeh

Açık

Türk

et al. 2018

GMJ

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Objective: Natural products with antimicrobial effect are currently investigated in order to eliminate the use of synthetic antibiotics that cause the resistance of microorganisms. This study determines the antibacterial, antioxidant activity, and also the cytotoxic and apoptotic effect of Satureja khuzestanica on various cancer cell lines. Materials and methods: The individual constituents were identified by their identical retention indices referring to known compounds from the literature. Methanol and ethanol extracts of S. khuzestanica (25 and 50 mg/mL) were screened against four Gram-positive and four Gram-negative bacteria. The plant extracts were tested for their antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. The interaction between the extracts and plasmid DNA were analyzed by agarose gel electrophoresis. In addition, the cytotoxic activity of the extracts was evaluated on MCF-7, DLD-1, osteosarcoma, and fibroblast cell line. Finally, gene expression of caspase-3, Bax and Bcl-2 were investigated by real-time PCR. Results: Our result indicated that both extracts showed good inhibitory activity against both Gram-negative (Escherichia coli ATCC 35218) and positive (Bacillus subtilis ATCC 6633) bacteria. In addition, the methanol extract of S. khuzestanica had strong antioxidant activity (IC50=37.0±0.3 μg/mL). The extracts showed a strong effect on plasmid DNA. The methanol extract of S. khuzestanica showed a good concentration-dependent cytotoxicity. Finally, IC50=47.00a±1.21μg/mL, ethanol, and water extract had apoptotic effect in MCF-7 cell line. Discussion: MCF-7 was detected as the most sensitive cell line therefore further studies should be done on this plant extract as a potential anticancer agent against breast cancer.

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Antimicrobial activity of Satureja hortensis L. essential oil against pathogenic microbial strains

Cited by 56 publications

References 32 publications

Efficacy of Essential Oils From Some African Spices Against Two Strains of Bacillus cereus Isolated From Vegetable Salad

Efficacy of Essential Oils From Some African Spices Against Two Strains of Bacillus cereus Isolated From Vegetable Salad

Antimicrobial Effect of Aqueous and Ethanolic Extracts of Satureja Bachtiarica on Some Pathogenic Bacteria in Vitro

Antimicrobial, antioxidant, cytotoxic and apoptotic activities of Satureja khuzestanica

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