Antimicrobial activity of puffball(Bovistella radicata) and separation of bioactive compounds

Ye, Yong; Liu, Kun; Zeng, Qinghua; Zeng, Qingmei

doi:10.1186/s13568-017-0402-5

Cited by 7 publications

(8 citation statements)

References 38 publications

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“…Pat) had remarkable antifungal activities. These data are consistent with previous ndings on the minimum inhibitory concentrations (MICs) and zone of inhibition (ZOI) of B. radicata fermentation [20].…”

Section: Discussionsupporting

confidence: 93%

“…Although no longer edible in their mature state (because of their powdery consistency), these puffballs have been shown to be a source of active compounds of various biological activities. Puffballs are believed to have several therapeutic properties when used medicinally: hemostasis [17], cough relief [18], suppression of cell division, and antitumor [19] and antimicrobial [20] properties. Petrović The aim of the present study was to evaluate the antimicrobial activity of Griseococcin(1) extracted from B. radicata fermentation broth.…”

Section: Introductionmentioning

confidence: 99%

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Griseococcin(1) from Bovistella radicata (Mont.) Pat and antifungal activity

Zeng

2020

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BackgroundTo evaluate the antimicrobial and microbicidel activity of B. radicata fermentation broth, the broth was purified by DEAE-cellulose and sephadex LC-20 column. The compounds were submitted to spectral analyses (HPLC, FT-IR, 1D and 2D NMR etc.). ResultsThe purified compounds were identified as the Griseococcin(s) which were naphthoquinone derivatives, the Chemical formula and MW of Griseococcin(1) was determined as C37O10H43N and 661Da. only Griseococcin(1) has good antimicrobial activity among the Griseococcin(s). The zone of inhibition (ZOI), minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) or minimum fungicidal concentration (MFC) of Griseococcin(1) were used to investigate the antimicrobial activity. Antifungal activity of Griseococcin(1) was significant, especially for main pathogenic fungus Trichophyton rubrum and Trichophyton mentagrophytes, MFC/MIC of Griseococcin(1) was 1, while MFC/MIC of postive control was greater than 4, the fungicidal effect of Griseococcin(1) was better than that of positive control.ConclusionsIn this paper, the secondary metabolite compound Griseococcin(1) from B. radicata was purified. The purified compound can restrain main pathogens (T. rubrum and T. mentagrophytes) leading to tinea pedis. The antifungal activity of Griseococcin(1) was similar to that of the positive control and the fungicidal effect of Griseococcin(1) was better than that of positive control, it might be suitable for pharmaceutical industries.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Griseococcin(1) from Bovistella radicata (Mont.) Pat and antifungal activity

Zeng

2020

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“…Puffballs are believed to have several therapeutic properties when used medicinally: hemostasis [16] , cough relief [17] , suppression of cell division, and antitumor [18] and antimicrobial [19] properties. Petrović P, et al(2016) reported noticeable antimicrobial activity diversity for the methanol extracts obtained from Handkea utriformis (HU), H. excipuliformis (HE), and Vascellum pratense (VP) [20] . The spore powder of HFTU-PB1 has been used traditionally as a folk remedy for tinea pedis.…”

Section: Introductionmentioning

confidence: 99%

Screening of Antifungal Substances from Bovistella Radicata (Mont.) Pat and Their Antifungal Effect

Zeng

Liu

et al. 2020

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Background To analyse the antifungal active compounds in B. radicata alcohol extract, the alcohol extract was purified by column chromatography (macroporous resinD-101) and the active compounds was named as SPAF-1(the spore powder active fraction). Results Alcohol extracts and SPAF-1 were submitted to GC-MS analysis, there were two characteristic peaks(peak1 and peak2) in Gas chromatogram. By comparing in NIST, the compound were 2-propyl-1-pentanol corresponding to peak1 and decanal, n-decanol and 2E-decanol corresponding to peak2. The main constituents were decanal(24.3%) n-decanol(27.9%), 2E-decenol (21.2%) and 2-Propyl-1-pentanol (13.6%). Their MIC values were 62.5 µg/ml, 31.2 µg/ml, 31.2 µg/ml and 250 µg/ml against T. rubrum respectively. Furthermore, transmission electron microscope (TEM) analysis showed altered surface morphology in the majority of T. rubrum cells after treatment with SPAF-1. Conclusions In this paper, we successfully separate SPAF-1 from alcohol extract of B. radicata. The antifungal effect of SPAF-1 is similar to positive control, the main component were decanal, n-decanol, 2E-decenol and 2-Propyl-1-pentanol, the anti-tinea pedis effect of them was obvious.

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“…In Nigeria puffball mushrooms popularly known as ‘Isu or Iso aparo in Yoruba language are part of culinary delicacies with several of the species of puffball mushrooms consumed locally (Oso 1977 ). Puffball are known especially for their nutritive component and a lot of them have also been reported for biological activities including blood glucose lowering activities (Kivrak et al 2014 ; Lee et al 2017 , 2018 ; Ye et al 2017 ). However, accurate identification of these local mushrooms is lacking.…”

Section: Introductionmentioning

confidence: 99%