Antimicrobial Activity of Plant Extracts against Bacillus Subtilis, Staphylococcus Aureus and Escherichia Coli

Abstract

Local herbs have many potential that may be active with antimicrobial activity. A screening was conducted with 11 species of herbs collected in UiTM Pahang Forest Reserve.Epipremnumsp.,Zingibersp.Tetracera indica, Tectaria crenata, Piper stylosum, Homalomena propinque, Goniothalamus sp., Elephantopus scaber, Mapania patiolale, Melastomasp.,Stemona tuberosa, Phullagathis rotundifolia, Thotea grandifoliaandSmilaxsp. were extracted with methanol to obtain their crude. The agar diffusion method using blank disc of 6 mm diameter were loaded with 1000 µg/mL of methanol crude and applied to the inoculate plate was used to assess the antimicrobial activity against two gram positive bacteria (Bacillus subtilisandStaphylococcus aeureus) and one gram negative bacteria (Escherichia coli). The results evaluated as the diameter of the inhibition zone of microbial growth, showed that all the extracts were active against gram-positive bacteria and gram-negative bacteria. The extract ofStemona tuberosewas found to be the most active against theE. coliandS. aeureuswhilePiper stylosumactive againstB. subtilis.

Unveiling Chemical, Antioxidant and Antibacterial Properties of Fagonia indica Grown in the Hail Mountains, Saudi Arabia

“…These results were in agreement with those of Anil et al [ 27 ], who suggested that the diverse antibacterial activity of the plant extract is explained by the presence of several bioactive components, such as saponins, flavonoids and alkaloids. Previous research found that the alcoholic extract of many plants such as Piper stylosum , Epipremnum sp., Tetracera indica , Zingiber sp., Tectaria crenata , Goniothalamus sp., Homalomena propinque , Smilax sp., Elephantopus scaber , Mapania patiolale , Melastoma sp., Phullagathis rotundifolia , Stemona tuberosa and Thotea grandifolia could inhibit the growth of Bacilus subtilis [ 45 ].…”