Antimicrobial activity of PLA/PEG nanofibers containing terpinen‐4‐ol against <i>Aggregatibacter actinomycetemcomitans</i>

Nepomuceno, Neymara C.; Barbosa, Mariângela A.; Bonan, Roberta Ferreti; Oliveira, Juliano Elvis de; Sampaio, Fábio Correia; Medeiros, Eliton S.

doi:10.1002/app.45782

Cited by 24 publications

(21 citation statements)

References 33 publications

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“…On the other hand, a number of studies have designed PLA/PEG film contained antimicrobial agents for various applications. Barbosa et al demonstrated that the addition of terpinen-4-ol to PLA/PEG reduces the viable Aggregatibacter actinomycetemcomita cells of biofilm 33 . Another study indicated that the incorporation of magnesium oxide nanoparticles into PLA/PEG enhances the antibacterial efficacy of the film 34 .…”

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confidence: 99%

Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Bardania

Mahmoudi

Bagheri

et al. 2020

Sci Rep

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To eliminate the microbial infection from an injury site, various modalities have been developed such as dressings and human skin substitutes. However, the high amount of reactive oxygen species, microbial infection, and damaging extracellular matrix remain as the main challenges for the wound healing process. In this study, for the first time, green synthesized silver nanoparticles (AgNPs) using Teucrium polium extract were embedded in poly lactic acid/poly ethylene glycol (PLA/PEG) film to provide absorbable wound dressing, with antioxidant and antibacterial features. The physicochemical analysis demonstrated, production of AgNPs with size approximately 32.2 nm and confirmed the presence of phytoconstituents on their surface. The antibacterial assessments exhibited a concentration-dependent sensitivity of Staphylococcus aureus and Pseudomonas aeruginosa toward biosynthesized AgNPs, which showed a suitable safety profile in human macrophage cells. Furthermore, oxidant scavenging assays demonstrated exploitation of plant extract as a reducing agent, endows antioxidant activity to biogenic AgNPs. The formation of PLA/PEG nanofilm and entrapment of AgNPs into their matrix were clearly confirmed by scanning electron microscopy. More importantly, antibacterial examination demonstrated that the introduction of biogenic AgNPs into PLA/PEG nanofibers led to complete growth inhibition of P. aeruginosa and S. aureus. In summary, the simultaneous antioxidant activity and antimicrobial activity of the novel biogenic AgNPs/PLA/PEG nanofilm showed its potential for application as wound dressing.activities of porous Ag nanofilm were investigated. We anticipate that the Ag nanofilm wound dressing with free radical scavenging capacity would enhance the wound healing process. Moreover, this study proposed a low-cost and facile method for preparation of Ag nanofilm as a potential wound dressing. Materials and MethodsMaterials. Methanol (CH 3 OH, 99.9%), silver nitrate (AgNO 3 ), nutrient agar, ferric chloride hexahydrate (FeCl 3 , 6H 2 O), iron (II) sulfate tetrahydrate (FeSO 4 .4H 2 O), dichloromethane, polyethylene glycol 300, ascorbic acid and Mueller-Hinton agar (MHA) were purchased from Merck (Germany). 2,2-diphenyl-1-picrylhydrazyl (DPPH), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and 2,4,6-Tripyridyl-s-Triazine (TPTZ) were purchased from Sigma (Germany) and poly lactic acid (PLA) from Zhejiang Hisun Biomaterials (China). The RAW264 macrophage cell line was purchased from National Cell Bank, Pasteur Institute of Iran, Iran and Dulbecco's Modified Eagle's Medium (DMEM), Fetal Bovine Serum (FBS), 1% Penicillin-Streptomycin from Gibco (Paisley, UK). All aqueous solutions were prepared using double distilled water. All reagents used were of analytical grade. Clinical strains of Pseudomonas aeruginosa 38 and Staphylococcus aureus 39 bacteria were isolated from hospitalized patients by our group, which have been characterized as antibiotic-resistant strains.Extract preparation. T.polium is a wild-growing...

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confidence: 99%

Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Bardania

Mahmoudi

Bagheri

et al. 2020

Sci Rep

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“…2017; Nepomuceno et al . 2018). Solution blow spinning (SBS) is a technique for producing micro‐ and nanofibres with several applications for the controlled release of bioactive compounds (Bonan et al .…”

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confidence: 99%

“…2015; Nepomuceno et al . 2018). Therefore, the focus of this work was to evaluate the effect of different concentrations of the essential oils from O. basilicum and O. gratissimum on the physicochemical, antifungal and antimicotoxygenic properties of poly(lactic acid) nanofibres against the fungi Aspergillus ochraceus and Aspergillus westerdjikiae .…”

Section: Introductionmentioning

confidence: 99%

Antifungal and physicochemical properties of Ocimum essential oil loaded in poly(lactic acid) nanofibers

Brandão

Cardoso

Batista

et al. 2022

Letters in Applied Microbiology

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Significance and Impact of the Study: The continuous and improper application of synthetic fungicides can favour the selection of resistant OTA-producing fungi, and it can result in the induction of the biosynthesis of this toxic substance. In addition, it can cause adverse health effects for consumers. Essential oils are chemically safe and acceptable to consumers. The essential oils from Ocimum basilicum and Ocimum gratissimum encapsulated in lactic polyacid nanofibres hold promise for the control of fungal contamination and the inhibition of the production of ochratoxin A (OTA).

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“…Researchers have been worked about the development antibacterial PLA-based electrospun materials using chitosan and silver [43], thymol [44], propolis [45], silver and vitamin E [46], copaiba (Copaifera sp.) oil [47], tetracycline hydrochloride and chitosan [48], cinnamon essential oil [49], thymoquinone [50], tea tree and manuka oil [51], carvacrol [52], clary sage and black pepper [53], terpinen-4-ol [54], epidermal growth factor [55], quercetin [56], Nigella sativa herbal extract [57], Eucalyptus essential oil [58], silver diclofenac complex [59], chitosan, black pepper essential oil and limonene [60], selenium and clarithromycin [61], cefazolin [62], silver nanoparticles [63], zinc oxide [64], thyme essential oil [65], zenian (Carum copticum) essential oil [66], birch bark triterpene extract [67], Capparis spinosa L. extracts [68], Plumbago europaea plant extract [69], curcumin [70], babassu oil [71], and cypress (Cupressus sempervirens L.) essential oil [72].…”

Section: Introductionmentioning

confidence: 99%

An In Vitro Study of Antibacterial Properties of Electrospun Hypericum perforatum Oil-Loaded Poly(lactic Acid) Nonwovens for Potential Biomedical Applications

et al. 2021

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The growth of population and increase in diseases that cause an enormous demand for biomedical material consumption is a pointer to the pressing need to develop new sustainable biomaterials. Electrospun materials derived from green polymers have gained popularity in recent years for biomedical applications such as tissue engineering, wound dressings, and drug delivery. Among the various bioengineering materials used in the synthesis of a biodegradable polymer, poly(lactic acid) (PLA) has received the most attention from researchers. Hypericum perforatum oil (HPO) has antimicrobial activity against a variety of bacteria. This study aimed to investigate the development of an antibacterial sustainable material based on PLA by incorporating HPO via a simple, low-cost electrospinning method. Chemical, morphological, thermal, thickness and, air permeability properties, and in vitro antibacterial activity of the electrospun nonwoven fabric were investigated. Scanning electron microscopy (SEM) was used to examine the morphology of the electrospun nonwoven fabric, which had bead-free morphology ultrafine fibers. Antibacterial tests revealed that the Hypericum perforatum oil-loaded poly(lactic acid) nonwoven fabrics obtained had high antibacterial efficiency against Escherichia coli and Staphylococcus aureus, indicating a strong potential for use in biomedical applications.

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Antimicrobial activity of PLA/PEG nanofibers containing terpinen‐4‐ol against Aggregatibacter actinomycetemcomitans

Cited by 24 publications

References 33 publications

Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Antifungal and physicochemical properties of Ocimum essential oil loaded in poly(lactic acid) nanofibers

An In Vitro Study of Antibacterial Properties of Electrospun Hypericum perforatum Oil-Loaded Poly(lactic Acid) Nonwovens for Potential Biomedical Applications

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