Antimicrobial activity of Luma apiculata against resistant strains of Staphylococcus aureus and Klebsiella pneumoniae

Abstract

Bacterial resistance is the mechanism through which the bacteria can decrease the action of antimicrobial agents. Studies have reported an antimicrobial action of Luma apiculata, however, there are no in vitro studies demonstrating the effect of this plant against multidrug-resistant bacteria. The objective of this study was to explore the antimicrobial properties of alcoholic extract of Luma apiculata against standard multi-resistant bacterial pathogens. Multidrug-resistant strains of Staphylococcus aureus ATCC 43300 (MRSA) and Klebsiella pneumoniae ATCC BAA 1705 (CRKP) were incubated independently for 72 h at 37ºC with different concentrations (1-10,000 µg/mL) of alcoholic extract of Luma apiculata. The growth curve was studied up to 72 hours. The minimum inhibitory concentration (MIC) was determined by broth macrodilution method. The agar diffusion method was used to examine bacteriostatic activity and bactericidal activity was evaluated by a commercial kit and analyzed by flow cytometry. Analyses of chromatographic data, as well as mass spectrometry, were performed to determine the main compounds present in the extracts. Luma apiculata leaves extracts showed bactericidal activity and was effective attenuating growth in both bacterial strains. Thus, Luma apiculata could represent a natural alternative against multidrug-resistant bacterial strains. However, further studies should be undertaken to test their clinical utility.

Innovative Integration of Arrayan (Luma apiculata) Extracts in Chitosan Coating for Fresh Strawberry Preservation

“…Pseudomonas aeruginosa and St. aureus, establishing for the latter an IC50 (median inhibitory concentration) between 0.354 and 0.385 mg/mL [20]. On the other hand, Treulen et al (2019) established the MIC of an Arrayan extract for St. aureus between 1 and 10 mg/mL, consistent with our study, where a dose used for BC of 0.8% w/v (8 mg/mL) was established [34]. In a similar manner, Araya-Contreras et al ( 2019) determined that hexane Arrayan extracts showed antibacterial activity against St. aureus, St. epidermidis, I. saprophyticus, Enterococcus sp., A. baumanii, Pseudomona aeruginosa, and E. coli at concentrations greater than 100 µg/mL [19].…”

Staphylococcus aureus y Streptococcus pneumoniae: INFECCIONES NOSOCOMIALES QUE REPRESENTAN UNA AMENAZA APREMIANTE PARA LA SALUD PúBLICA

“…(i) Prueba de sensibilidad de S. aureus a cefoxitina (discos con 30 ug); (ii) Prueba de sensibilidad a vancomicina. Prueba E (Epsilometer-Test) para la cuantificación de la susceptibilidad de los microorganismos, determinando la concentración mínima inhibitoria (MIC)(Treulen et al, 2019).…”