Antimicrobial activity of highly stable silver nanoparticles embedded in agar–agar matrix as a thin film

Ghosh, Somnath; Kaushik, Rajeev; Nagalakshmi, K.; Hoti, S.L.; Menezes, Godfred Antony; Harish, Belgode Narasimha; Vasan, H. N.

doi:10.1016/j.carres.2010.08.001

Cited by 102 publications

(53 citation statements)

References 43 publications

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“…The antibacterial effect of silver nanoparticles on S. aureus was comparable to other studies examining the biological effects of silver colloids [5,10,17]. Taking similar particle sizes into account, a comparable antibacterial effect between laser-generated and chemically synthesized AgNP can be assumed.…”

Section: Antibacterial Activity Of Nanoparticlessupporting

confidence: 65%

“…Silver nanoparticles used in antibacterial implant coatings and surfaces are often embedded in composites or hydrogels, which release the silver ions over time to the adjacent area [8,9]. Unfortunately, high concentrations of antibacterial silver ions also exhibit cytotoxic effects on eukaryotic cells, making it difficult to establish a possible therapeutic window [10][11][12]. The cytotoxic effects of silver nanoparticles on eukaryotic cells are described in many studies and the particle concentrations, which cause at least moderate cytotoxicity depend very much on the type of cells used [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

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Alloying colloidal silver nanoparticles with gold disproportionally controls antibacterial and toxic effects

et al. 2013

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Elemental silver nanoparticles are an effective antibacterial substance and are found as additive in various medical applications. Gold nanoparticles are used due to their optical properties in microscopy and cancer therapy. These advantages might be combined within alloyed nanoparticles of both elements and thereby open new fields of interest in research and medical treatment. In this context, laser ablation of solid alloys in liquid gives access to colloidal silver-gold alloy nanoparticles with a homogeneous ultrastructure. Elemental and alloy silver-gold nanoparticles with increasing molar fractions of silver (50, 80, and 100 %) were produced and stabilized with citrate or albumin (BSA). Particles were embedded in agar at concentrations of 3-100 μg cm −3 and tested on clinical relevant Staphylococcus aureus regarding their antibacterial properties. Cytotoxic effects were measured within the same particle concentration range using human gingival fibroblasts (HGFib). As expected, a reduced fraction of silver in the nanoalloys decreased the antibacterial effect on S. aureus according to the evaluated minimal inhibitory concentrations. However, this decrease turned out stronger than expected by its relative mass per particle, due to the electrochemical, disproportionally high effect of gold on the bioresponse to silver within silver-gold nanoalloy particles.BSA was able to stabilize all colloids and maintain antibacterial activity, whereas sodium citrate reduced antibacterial effects and cytotoxicity even at high nanoparticle concentrations. The alloying of silver with gold by laser ablation in liquid produced nanoparticles with both reduced antibacterial and cytotoxic properties in comparison to silver nanoparticles but still retains the application spectrum of both elements combined in one colloid. In particular, alloying with gold may render silver nanoparticles more biocompatible, and allows bioconjugation via established thiol chemistry.

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Section: Antibacterial Activity Of Nanoparticlessupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Alloying colloidal silver nanoparticles with gold disproportionally controls antibacterial and toxic effects

et al. 2013

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“…Based on Table 1, the YM value of the blank seaweed film was 2.15 GPa, which was higher than those seaweed-derived polymers such as alginate (1.88 GPa), carrageenan (1.1 GPa), and agar (1.3 GPa) (Ghosh et al 2010;Shankar et al 2015. However, the addition of OPS nanofillers up to 5% did not lead to significantly different YM values in the composite film as compared to the control film.…”

Section: Mechanical Properties Of Bio-nanocomposite Filmsmentioning

confidence: 90%

Oil Palm Shell Nanofiller in Seaweed-based Composite Film: Mechanical, Physical, and Morphological Properties

et al. 2017

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Composite films that utilize seaweed as a matrix and oil palm shell (OPS) nanoparticles as a reinforcing material were developed. The effects of loading OPS nanoparticle (0%, 1%, 5%, 10%, 20%, and 30%) into seaweed films were determined by analyzing the physical, mechanical, and morphological properties of the films. The seaweedbased film incorporated with OPS nanoparticles at a high concentration (20% w/w) achieved the highest tensile strength (44.8 MPa) and Young's Modulus (3.13 GPa). However, the film's hydrophobicity (contact angle = 47.3º) and percentage of elongation at break (2.10%) were reduced. Moreover, it was observed that excessive loading of nanofillers (> 20%) reduced the tensile strength and hydrophilicity of the film. This phenomenon was attributed to the agglomeration of OPS nanoparticles and the formation of large voids on the film surface. Thus, the relative effectiveness of the various tested nanofiller contents in enhancing the mechanical strength of the composite film were found to be ranked in the following order: 20%, 10%, 5%, 30%, and 1%.

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“…La plata es uno de los aditivos con características antimicrobianas mayoritariamente utilizado en diversos materiales poliméricos como el agar (Ghosh et al, 2010;Rhim et al, 2013), alcohol de polivinilo (Bryaskova et al, 2010;Sedlarik et al, 2010), gelatina (Kanmani y Rhim, 2014) y mezclas de quitosan-celulosa (Lin et al, 2015), almidón-arcilla (Abreu et al, 2015) o quitosan-glutaraldehido (Vimala et al, 2011) para aplicaciones de empacado de alimentos (Martínez-Abad et al, 2012). Sin embargo, no hay casi estudios concernientes al desarrollo de BP a base de GBA cargadas de AgNPs y su comportamiento físico-mecánico.…”

Section: Introductionunclassified

Biopelículas de Gelana Cargadas con Nanopartículas de Plata: Propiedades Físico Mecánicas, Antimicrobianas y de Barrera

2017

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ResumenSe elaboraron biopelículas a base de gelana de bajo acilo cargadas con nanopartículas de plata (AgNPs) evaluando sus propiedades físico-mecánicas, antimicrobianas y de barrera, después de un almacenamiento de 3 semanas. La actividad antimicrobiana fue evaluada frente a bacterias de origen alimentario (Escherichia coli y Staphylococcus aureus) y dos hongos (Aspergillus sp y Penicillium sp). Los resultados permitieron determinar que el tiempo de almacenamiento influye en todas las propiedades evaluadas. Al incrementar el tiempo de almacenamiento se apreció una disminución en la resistencia a la tensión y en la transparencia. Además se presentó un incremento en el poder antimicrobiano y la permeabilidad al vapor de agua. Este comportamiento probablemente obedece a la lixiviación de iones plata, lo cual disminuye el número de enlaces presentes en la matriz polimérica. Tales resultados son prometedores y sugieren la posibilidad de utilizar biopelículas a base de gelana de bajo acilo como sistema de vehiculización de AgNPs para su liberación gradual manteniendo un efecto antimicrobiano sostenido. Palabras clave: biopelículas; goma gelana; nanopartículas de plata; permeabilidad; resistencia a la tensión Biofilms of Gellan Loaded with Silver Nanoparticles: Physico Mechanical, Antimicrobials and Barrier Properties AbstractBiofilms based on low acyl gellan loaded with silver nanoparticles (AgNPs) were tested, evaluating their physical-mechanical, antimicrobial and barrier properties after 3 weeks of storage. The antimicrobial activity was evaluated against food-borne bacteria (Escherichia coli and Staphylococcus aureus) and two fungi (Aspergillus sp and Penicillium sp). The results showed that the storage time influence all the evaluated properties. Increasing the storage time decreases the tensile strength and transparency values. Also, the antimicrobial power and the water vapor permeability increased. This behavior is probably due to the leaching of silver ions, which decreases the number of bonds present in the polymer matrix. Such results are promising and suggest the possibility of using biofilms based on low acyl gellan as a system for the vehiculation of AgNPs for their gradual release while maintaining a sustained antimicrobial effect.

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Antimicrobial activity of highly stable silver nanoparticles embedded in agar–agar matrix as a thin film

Cited by 102 publications

References 43 publications

Alloying colloidal silver nanoparticles with gold disproportionally controls antibacterial and toxic effects

Alloying colloidal silver nanoparticles with gold disproportionally controls antibacterial and toxic effects

Oil Palm Shell Nanofiller in Seaweed-based Composite Film: Mechanical, Physical, and Morphological Properties

Biopelículas de Gelana Cargadas con Nanopartículas de Plata: Propiedades Físico Mecánicas, Antimicrobianas y de Barrera

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