Antimicrobial activity of Enterococcus faecium EF 55 against Salmonella Enteritidis in chicks

Levkut, M.; Pistl, J.; Lauková, Andrea; Révajová, Viera; Herich, R.; Ševčíková, Zuzana; Strompfová, Viola; Szabóová, R.; Kokinčáková, T.

doi:10.1556/avet.57.2009.1.2

Cited by 27 publications

(17 citation statements)

References 22 publications

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“…In vivo, the inhibitory effect of E. faecium EF55 against different bacteria, including Salmonella Enteritidis was demonstrated in our previous study (8). Recently, beneficial influence of EF55 strain on the production of intestinal mucin has been shown in chickens (9).…”

Section: Introductionmentioning

confidence: 74%

Effect of Enterococcus faecium EF 55 on morphometry and proliferative activity of intestinal mucosa in broilers infected with Salmonella Enteritidis

Ševčíková

Blanár

Lauková

et al. 2016

Journal of Veterinary Research

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Introduction: The present study aimed to investigate the effect of Enterococcus faecium EF55 on chickens, as well as its influence on proliferative activity of epithelial intestinal cells after infection with Salmonella enterica serovar Enteritidis phage type 4 (SE PT4). Moreover, the length and area of duodenal and jejunal villi of the birds were examined. Material and Methods: A pool of 80 birds was divided randomly into four groups. Probiotic group (EF) and Salmonella + probiotic group (EFSE) received E. faecium EF55 (10 9 CFU -3 g per group/day) during 22 d. Salmonella group (SE) and EFSE group were infected with Salmonella Enteritidis PT4 (10 8 CFU in 0.2 mL PBS) in a single dose per os on day four of the experiment, whereas control birds (C group) received only 0.2 mL of PBS on that day. Samples were taken on the 4 th and 18 th day post infection. Results: Supplementation of feed with E. faecium EF 55 confirmed its selective antibacterial activity against SE PT4. The chickens infected with SE PT4 and fed E. faecium EF55 supplemented diet showed increased proliferative activity of enterocytes in the jejunum in both samplings. Applied probiotic strain demonstrated positive impact on intestinal morphometry in the jejunum of both non-infected groups and in Salmonella-infected chickens. In the latter group, the beneficial effect of E. faecium EF 55 was manifested by more efficient tissue turnover in the jejunum.

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Section: Introductionmentioning

confidence: 74%

Effect of Enterococcus faecium EF 55 on morphometry and proliferative activity of intestinal mucosa in broilers infected with Salmonella Enteritidis

Ševčíková

Blanár

Lauková

et al. 2016

Journal of Veterinary Research

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“…It has been repeatedly confirmed that enterocins unrelated to the source of the enterocin-producing strain are effective. Poultry farming represents a livestock sector where probiotic preparations are frequently used and where probiotic bacteria (mainly of the species E. faecium) have already been successfully applied (Lauková et al 2003, Levkut et al 2009). In our previous work inhibitory effect against coliform bacteria was shown not only in vitro (Lauková et al 1993), but also in vivo, e.g.…”

Section: Discussionmentioning

confidence: 99%

Escherichia coli strains from ostriches and their sensitivity to antimicrobial substances

Ščerbová¹,

Lauková²

2016

Polish Journal of Veterinary Sciences

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Ostriches are bred especially for their high-quality meat. There is a lack of knowledge concerning the ostrich's microflora. Escherichia coli is a commensal microorganism of the poultry intestine, ostriches included. However, some strains may become pathogenic. This study was therefore undertaken to detect coliform bacteria in ostrich faeces and to test their antibiotic profile and sensitivity to enterocins. Faeces (n=54, 18 mixture samples from 3 different age groups of 140 ostriches) were sampled to isolate coliform bacteria. The counts of coliform bacteria varied from 5.69 ± 2.4 log10 CFU/g to 5.73 ± 2.4 CFU/g. Pure colonies were identified using MALDI-TOF MS mass spectrometry and confirmed by phenotypization. Seventy-one strains were allotted to the species E. coli. Sixty-four of those 71 strains caused hemolysis. They were mostly polyresistant to antibiotics. Thirty-two polyresistant strains of E. coli were sensitive to enterocins. These strains were most sensitive to Ent 9296 (26 strains). Moreover, Ent EM41 produced by E. faecium EM41 (isolated from ostrich faeces) inhibited the growth of 20 strains, reaching activity of 100 AU/ml. Our results indicate the possibility of enterocins being used for prevention/reduction of coliforms. Of course, in vivo studies are also being processed.

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“…Duodenal intraepithelial lymphocytes were isolated by modification some methods and published in detail by Levkut et al (2009). Mononuclear cells from blood and intestine were separated over Histopaque-1077 gradient sedimentation (Sigma, Germany).…”

Section: Flow Cytometrymentioning

confidence: 99%

Duodenal morphology and immune responses of broiler chickens fed low doses of deoxynivalenol

et al. 2013

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Morphometry and flow cytometry for intraepithelial lymphocyte phenotyping were used to determine the changes in duodenal mucosae after administration of low doses of deoxynivalenol in chickens. Moreover, functions of phagocytes and immunocompetent cells in peripheral blood were evaluated by flow cytometry. In total, sixty chickens of Ross hybrid broilers 308 were used in this experiment. Two experimental groups of 20 birds were continually fed for 14 days a diet containing deoxynivalenol at a dose of 1 and 3 mg·kg -1; 20 birds of the control group were fed uncontaminated diet. Morphometry showed only tendency to decrease the height of villi and surface area of duodenal mucosae in chickens fed the diet supplemented with 3 mg·kg -1 deoxynivalenol. Phenotyping of intraepithelial lymphocytes showed a decrease of CD45+ (P < 0.034) in duodenum of birds fed diets supplemented with deoxynivalenol. Examination of white blood cells showed a decrease of monocytes (P < 0.020) in chickens fed 3 mg·kg -1 of deoxynivalenol. Both experimental groups revealed higher metabolic burst of peripheral blood heterophils (P < 0.001). Phenotyping of immunocompetent cells showed an increase (P < 0.003) of CD3+ and a decrease (P < 0.001) of MHC II+ cells in peripheral blood of chickens fed with 3 mg·kg -1 dose of deoxynivalenol. The experimental feeding of chickens with deoxynivalenol resulted in immunomodulation of immunocompetent cells in duodenum and blood with mild atrophy of intestinal villi, mainly after the feeding of the dose of 3 mg·kg -1 . We proved that even low doses of deoxynivalenol can cause changes in haemathological, immunological and morphological profiles already during two weeks, and lead to the activation of compensatoryadaptive mechanisms with unfavourable impact on health and performance of birds.

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Antimicrobial activity of Enterococcus faecium EF 55 against Salmonella Enteritidis in chicks

Cited by 27 publications

References 22 publications

Effect of Enterococcus faecium EF 55 on morphometry and proliferative activity of intestinal mucosa in broilers infected with Salmonella Enteritidis

Effect of Enterococcus faecium EF 55 on morphometry and proliferative activity of intestinal mucosa in broilers infected with Salmonella Enteritidis

Escherichia coli strains from ostriches and their sensitivity to antimicrobial substances

Duodenal morphology and immune responses of broiler chickens fed low doses of deoxynivalenol

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