Antimicrobial Activity of Chitosan Film Forming Solution Enriched with Essential Oils; an in Vitro Assay

Abstract

The resistance of the bacteria and fungi to the innumerous antimicrobial agents is a major challenge in the treatment of the infections demands to the necessity for searching and finding new sources of substances with antimicrobial properties. The incorporation of the essential oils (EOs) in chitosan film forming solution may enhance antimicrobial properties. However, its use as the feeding additive in the poultry nutrition needs to clarify the product's activity against both pathogen and the useful microbes in the gastrointestinal tract. In the present study, we carried out an investigation and evaluated the antimicrobial activity of chitosan film forming solution incorporated with essential oils (CFs+EOs) against microbial strains including and. In three replicates, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of different treatments including: 1- essential oils (EOs), 2- chitosan film solution (CFs), and 3-chitosan film solution enriched with EOs (CFs+EOs) were determined against above mentioned microbes. The results indicated that the chitosan solution enriched with essential oils (CFs+EOs) is capable of inhibiting the bacterial and fungal growth even at the lowest concentrations. The MIC and MBC for all the antimicrobial agents against and were very low compared to the concentrations needed to inhibit the growth of useful bacteria, and. The antifungal activity of chitosan was enhanced as the concentration of EOs increased in the film solution. Chitosan-EOs complexes are the promising candidate for novel contact antimicrobial agents that can be used in animal feeds.

Basil Essential Oil: Composition, Antimicrobial Properties, and Microencapsulation to Produce Active Chitosan Films for Food Packaging

“…Although CH is known to possess antimicrobial activity, it was shown to be inactive against a serials of pathogenic and spoilage bacteria [ 52 , 53 , 54 ], probably because of different factors including experimental conditions (concentrations, pH, type of microorganism, and neighboring components) as well as its molecular properties (molecular weight, degree of deacetylation, and original source) [ 55 ]. In general, however, incorporation of the EOs conferred or enhanced antibacterial efficiency of CH films against different spoilage microorganisms and food-borne pathogens [ 56 , 57 , 58 ]. In this respect, Cristani et al (2017) [ 59 ], reported that the observed antimicrobial action can be attributed to the EO content in terpenes that affect the permeability and other functions of the bacterial membranes.…”

Antilisterial activity of chitosan‐based edible coating incorporating cell‐free supernatant fromPediococcus pentosaceus147 on the preservation of fresh cheese

“…The partially purified CFS was diluted and the antimicrobial activity was evaluated (Kana & Amir, 2017). Briefly, the CFS was twofold diluted with BHI broth.…”

Chitosan/Thyme Oil Systems as Affected by Stabilizing Agent: Physical and Antimicrobial Properties

“…The mutual interactions between chitosan polymer and active/stabilizing agents were studied by Fourier-transform infrared spectroscopy; obtained results for chitosan film and chitosan film enriched with 2 wt.% TEO are shown in Table 4 and Figure 1. As can be seen from the main absorption bands (Table 4), thyme oil exhibited some characteristic peaks, e.g., at 3390 cm −1 (O-H), 2961 cm −1 (C-H stretching of methyl and isopropyl groups on thymol phenolic structure), 1619 cm −1 (conjugated double bond of ring), 1457 cm −1 (CH deformation), and 1289 cm −1 (C-O-C) [26,27]. The FTIR spectrum of chitosan showed characteristic signals between 3500 and 3000 cm −1 related O-H and N-H bonds in the amino group.…”

“…Following chitosan modification with thyme EO, a peak at 2959 cm −1 was indicated. Newly emerged peaks in the wavelength range from 900 to 600 cm −1 refer to the potential interaction between chitosan and EO, i.e., incorporation of EO into the chitosan film [26]. A comparison of spectra in the selected interval (1800-400 cm −1 ) of the chitosan film and the chitosan film enriched with 2 wt.% TEO is shown in Figure 1.…”