Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L.

Karaman, İsa; Şahi̇n, Fikrettin; Güllüce, Medine; Öğütçü, Hatice; Şengül, Memnune; Adıgüzel, Ahmet

doi:10.1016/s0378-8741(03)00006-0

Cited by 392 publications

(269 citation statements)

References 10 publications

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“…Staphylococcus aureus was the most sensitive to essential oil, it was followed by Staphylococcus epidermitidis and some other The reported differences in susceptibility may be due to the differences in the cell wall composition and/or genetic content of their plasmids [32].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Activity of Mentha Pulegium Essential Oil Against Avian Isolated Esbl Producing Bacteria and Its Synergistic Potential With Antibiotics

Yahiaoui

Benameur

Ben-Mahdi

2017

Int J Pharm Pharm Sci

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Objective: The aim of this study was to evaluate the antibacterial effect of Algerian Mentha pulegium essential oil against extended-spectrum β-lactamases (ESBL) producing bacteria isolated from avian livestock. The synergistic interactions between conventional antibiotics and Mentha pulegium essential oil were assessed. Methods:Essential oil composition was determined by gas chromatograph-flame ionization detector (GC/FID), gas chromatograph-mass spectrometer (GC/MS) analysis.Antibacterial activity of Mentha pulegium essential oil against ESBL producing bacteria was investigated by disc diffusion assay. Minimum inhibitory concentration (MIC) of essential oil and it synergistic interaction with conventional antibiotics were determined by micro-broth dilution method and checkerboard test, respectively. Results:The results indicate that Mentha pulegium essential oil with a high amount of pulegone (88.78%) had high inhibitory activity against the tested strains and particularly displays a satisfactory action against the studied ESBL producing bacteria from animal origin, with a diameter ranging from 13 to 26 mm. Out of 51 combinations tested between essential oil and antibiotics 60.78 % showed total synergy, 13.72 % had presented a partial synergy. The best antibacterial activities were obtained with the combination of Mentha pulegium essential oil and cefazolin, cefotaxime and gentamycin. Conclusion:This study allowed concluding that Mentha pulegium essential oil showed not only satisfactory antibacterial properties, but also acts synergistically combined with conventional antibiotics, which make it a promising alternative to antimicrobial drugs; beside that, it might reduce the minimum effective dose of the drugs which minimizes their possible side effects.

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Section: Discussionmentioning

confidence: 99%

Antibacterial Activity of Mentha Pulegium Essential Oil Against Avian Isolated Esbl Producing Bacteria and Its Synergistic Potential With Antibiotics

Yahiaoui

Benameur

Ben-Mahdi

2017

Int J Pharm Pharm Sci

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“…O método de disco-difusão foi proposto por vários autores para verificar a atividade antimicrobiana de extratos vegetais, como Nascimento et al (2000) e Karaman et al (2003). Já Ostrosky et al (2008) mencionaram o método de microdiluição em caldo como sendo o mais confiável para avaliar agentes antimicrobianos, por fornecer resultados quantitativos e não ser influenciado pela velocidade de crescimento do micro-organismo.…”

Section: Palavras-chaveunclassified

Comparação de métodos para avaliação da atividade antimicrobiana e determinação da concentração inibitória mínima (cim) de extratos vegetais aquosos e etanólicos

et al. 2014

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Several studies have been conducted to discover new antimicrobial agents from plants to be used in pharmaceuticals, cosmetics and in the food industry. The lack of standardized methods for the evaluation of plant extracts with antimicrobial potential complicates the comparison of results. Taking into consideration the need to establish a method with consistent results to evaluate the antimicrobial activity of plant extracts, this study aimed to evaluate the antimicrobial activity of extracts of some plants from the Myrtaceae family on different micro-organisms based on a comparative assay with three methods that are commonly used for the assess antimicrobials. The methods used were broth microdilution, and agar diffusion by disc and well. The evaluated extracts were those of Psidium guajava, Myrciaria cauliflora, and Syzygium cumini for Grampositive and negative bacteria and yeast. In general, inhibition extracts promoted by the agar diffusion test by well was higher than the values obtained by disc, regardless of the plant extracts tested. However, the inhibitory activity of all micro-organisms was only possible to be determined with the microdilution broth method, which also presented the more reproducible results, and proved to be the most economic and reliable way to evaluate the antimicrobial activity of plant extracts in contrast with other methods. KEYWORDS:Myrtaceae; agar diffusion; disc diffusion; microdilution. RESUMO:Vários estudos estão sendo conduzidos para a descoberta de novos agentes antimicrobianos provenientes de plantas, para que possam ser utilizados em produtos farmacêuticos, cosméticos e na indústria alimentícia. A ausência de padronização de métodos utilizados para a avaliação de extratos vegetais com potencial antimicrobiano dificulta a comparação de resultados. Considerando a necessidade de estabelecer um método com resultados consistentes para avaliar a atividade antimicrobiana dos extratos vegetais, este trabalho propôs-se a avaliar a atividade antimicrobiana e determinar a concentração mínima inibitória de extratos de plantas da família Myrtaceae sobre diferentes micro-organismos, utilizando três méto-dos para avaliação de antimicrobianos. Os métodos empregados foram microdiluição em caldo e difusão em ágar por disco e poço. Foram avaliados os extratos de Psidium guajava, Myrciaria cauliflora e Syzygium cumini sobre bactérias Gram-positivas, negativas e levedura. Em geral, a inibição promovida pelos extratos no teste de difusão em ágar por poço foi maior do que os valores obtidos por disco, independentemente do extrato vegetal testado. Contudo, a atividade inibitória de todos os micro-organismos só pôde ser determinada com o método de microdiluição em caldo, que também apresentou os resultados mais reprodutíveis, e mostrou-se o mais econômico e confiável para se avaliar a atividade antimicrobiana de extratos vegetais quando comparado aos outros métodos. PALAVRAS-CHAVE:Myrtaceae; difusão em ágar; disco-difusão; microdiluição.Comparação de métodos para avaliação da atividade a...

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“…Controls with distilled water only were also conducted to assure that Tween 80 did not influence growth of the bacteria. Antibacterial activities were recorded by using the disc diffusion method of Karaman et al (2003), of which the content of M A and M B was 400 mg per disc, and that of ampicillin was 30 mg. One hundred ml of test organisms (10 6 colony forming units CFU/ml) were grown in nutrient broth media for 24 h and were spread over the surface of solid nutrient agar medium in Petri dish (9 cm in diameter). Filter paper discs (6 mm in diameter) loaded with each of the samples and ampicillin were placed on the surface of the nutrient agar and were incubated at 378C for 24 h, and then the diameters of inhibitory areas were determined by measurement in millimeters.…”

Section: Isolation Of M a And M Bmentioning

confidence: 99%

Comparative efficaciesin vitroof antibacterial, fungicidal, antioxidant, and herbicidal activities of momilatones A and B

Fukuta¹,

Xuan²,

Deba³

et al. 2007

Journal of Plant Interactions

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Momilactones A (M A ) and B (M B ) are phytoalexins derived from rice plant (Oryza sativa) and were considered to be a part of the mechanism of rice self-defense system. The present study was to evaluate the comparative efficacies in vitro of antibacterial, fungicidal, antioxidant, and herbicidal activities of M A and M B . In general, M B shows higher antifungal, antibacterial, and herbicidal action than M A , although its antioxidant property was less than M A . In herbicidal trial, the IC 50 values of M B against germination, shoot and root elongation of barnyardgrass and monochoria were 40.9, 45.5, and 27.5, and 27.1, 17.3, and 0.9 mg, respectively. For M A , these values were 40.3, 35.6, and 55.1, and 43.9, 24.3, and 0.5 mg, respectively. For antifungal activity, momilactone B (IC 50 : 1.2, 123.9, and 53.4 mg) exerted significantly greater inhibition than M A (IC 50 : 78.1, 198.1, and 95.3 mg) against Botrytis cinerea, Fusarium solani, Colletrotrichum gloeosporioides, respectively, except for Fusarium oxysporum that both M A and M B showed no marked difference. In addition, M B exhibited significantly stronger antibacterial activity than M A against Pseudomonus ovalis, Bacillus cereus, and Bacillus pumilus, whereas the inhibitory activity of the two compounds was similar against Escherichia coli. Both M A and M B exerted rather weak antioxidant activity (EC 50 was 783.9 and 790.7 mg, respectively), of which M A showed a slightly stronger antioxidant activity than M B . This study is the first to examine antifungal, antibacterial, and antioxidant activities of two phytoalexins, as well as their comparative efficacies against growth of the noxious weeds barnyardgrass and monochoria.

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Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L.

Cited by 392 publications

References 10 publications

Antibacterial Activity of Mentha Pulegium Essential Oil Against Avian Isolated Esbl Producing Bacteria and Its Synergistic Potential With Antibiotics

Antibacterial Activity of Mentha Pulegium Essential Oil Against Avian Isolated Esbl Producing Bacteria and Its Synergistic Potential With Antibiotics

Comparação de métodos para avaliação da atividade antimicrobiana e determinação da concentração inibitória mínima (cim) de extratos vegetais aquosos e etanólicos

Comparative efficaciesin vitroof antibacterial, fungicidal, antioxidant, and herbicidal activities of momilatones A and B

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