Antimicrobial Activity of a Bacteriocin Produced by Lactobacillus Plantarum 29V and Strain′S Viability in Palm Kernel Oil

François, Zambou Ngoufack

doi:10.11648/j.ijnfs.20130203.12

Cited by 12 publications

(10 citation statements)

References 19 publications

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“…Enterococcus faecium (LUM35, LUM38 and SOF45) strains showed the highest antimicrobial activity against food borne pathogens. Similar results were also reported with Enterococcus faecium (Araújo and Ferreira, 2013;Francois et al, 2013), also they observed the antimicrobial activity of Enterococcus faecium and Lb. plantarum against spoilage.…”

Section: Antibacterial Activitysupporting

confidence: 89%

Probiotic Properties of Lactic Acid Bacteria Isolated From Egyptian Salted Food

2017

Zagazig Journal of Agricultural Research

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A total of 12 autochthonous lactic acid bacteria (LAB) were isolated from a traditional homemade salted foods (n=14) in Egypt. The salted fish (n=2), mixed pickles (n=3) and mish (n=9) at one to 48 months of fermentation were collected from Sharkia Governorate (Zagazig, Menya-Elkmh, Belbeas, Fakous and Kafr-Suqr districts), North Sinai Governorate (El-Areesh and Rafah cities), Sohag, Luxor and Aswan cities. Identification process to species and strain level was accomplished by biochemical, morphological characteristics and 16S rDNA gene analysis. The isolates were evaluated according to safety, antibacterial and antibiotic susceptibility criteria. The most frequently observed genus identified by 16S rDNA sequencing analysis was Enterococcus faecium (SHM7, ELM33 and ELM38). The most predominant genus identified by biochemical were Lactobacillus rhamnosus < Lb. frumenti < Enterococcus faecium < Lb. plantarum. The highest percentages of antibiotic resistance were to cefotaxime and cefaclor (91.67%), and ampicillin, kanamycin and levofloxacin (83.33%). All the selected isolates were sensitive to tetracycline and penicillin (25 and 50%, respectively).The results also revealed that 33.33% of the isolates were haemolytic, while 66.67% were non-haemolytic and only one isolate (SHM4) was induced βhaemolytic activity. All the selected isolates showed important levels of antibacterial activity against tested food-borne pathogens (Salmonella enterica, Listeria monocytogenes and Escherichia coli). The results suggest the potential of the obtained isolates to improve the quality of these traditional fermented food products in Egypt.

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Section: Antibacterial Activitysupporting

confidence: 89%

Probiotic Properties of Lactic Acid Bacteria Isolated From Egyptian Salted Food

2017

Zagazig Journal of Agricultural Research

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“…18,21 Moreover, the low inhibitory effect of CeO 2 NPs on E. coli O157:H7 and that of both NPs on S. aureus in the monoculture tests was substantially enhanced by coculture with L. plantarum. As pointed out by François et al 15 and Sharif et al, 20 L. plantarum can effectively inhibit the growth of E. coli and S. aureus. Similarly, a Lactobacillus species was shown to be effective against S. aureus in two other studies.…”

mentioning

confidence: 87%

“…[14][15][16] Of these metabolites, bacteriocins are of particular interest because there have been several reports demonstrating their antimicrobial efficiency across a broad spectrum of microbes. [17][18][19] Although many bacteria can produce bacteriocins, L. plantarum is chosen in this study due to its Generally Regarded As Safe status.…”

Section: Introductionmentioning

confidence: 99%

“…Bacteriocins are proteinaceous antimicrobial compounds produced extracellularly during the stationary phase of bacterial culture. 15,17 Bacteriocins produced by Lactobacillus species are known as "plantaricins", and their stability varies depending on the specific physicochemical environment and Lactobacillus species used. 14,[19][20][21] In view of this, we intended to coculture each test bacteria with L. plantarum to evaluate the possible synergistic antibacterial effect by L. plantarum and as-synthesized CeO 2 NPs or Au/CeO 2 NPs.…”

Section: Introductionmentioning

confidence: 99%

“…For the coculture studies, a lactic acid bacterium, L. plantarum, reported to have antibacterial activity, 15,20 was cocultured along with each test pathogen. The observed …”

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confidence: 99%

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Cytotoxicity and antibacterial activity of gold-supported cerium oxide nanoparticles

Babu¹,

Anandkumar²,

Tsai³

et al. 2014

IJN

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Background Cerium oxide nanoparticles (CeO 2 ) have been shown to be a novel therapeutic in many biomedical applications. Gold (Au) nanoparticles have also attracted widespread interest due to their chemical stability and unique optical properties. Thus, decorating Au on CeO 2 nanoparticles would have potential for exploitation in the biomedical field. Methods In the present work, CeO 2 nanoparticles synthesized by a chemical combustion method were supported with 3.5% Au (Au/CeO 2 ) by a deposition-precipitation method. The as-synthesized Au, CeO 2 , and Au/CeO 2 nanoparticles were evaluated for antibacterial activity and cytotoxicity in RAW 264.7 normal cells and A549 lung cancer cells. Results The as-synthesized nanoparticles were characterized by X-ray diffraction, scanning and transmission electron microscopy, and ultraviolet-visible measurements. The X-ray diffraction study confirmed the formation of cubic fluorite-structured CeO 2 nanoparticles with a size of 10 nm. All synthesized nanoparticles were nontoxic towards RAW 264.7 cells at doses of 0–1,000 μM except for Au at >100 μM. For A549 cancer cells, Au/CeO 2 had the highest inhibitory effect, followed by both Au and CeO 2 which showed a similar effect at 500 and 1,000 μM. Initial binding of nanoparticles occurred through localized positively charged sites in A549 cells as shown by a shift in zeta potential from positive to negative after 24 hours of incubation. A dose-dependent elevation in reactive oxygen species indicated that the pro-oxidant activity of the nanoparticles was responsible for their cytotoxicity towards A549 cells. In addition, cellular uptake seen on transmission electron microscopic images indicated predominant localization of nanoparticles in the cytoplasmic matrix and mitochondrial damage due to oxidative stress. With regard to antibacterial activity, both types of nanoparticles had the strongest inhibitory effect on Bacillus subtilis in monoculture systems, followed by Salmonella enteritidis , Escherichia coli , and Staphylococcus aureus , while, in coculture tests with Lactobacillus plantarum , S. aureus was inhibited to a greater extent than the other bacteria. Conclusion Gold-supported CeO 2 nanoparticles may be a potential nanomaterial for in vivo application owing to their biocompatible and antibacterial properties.

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Probiotic Potential of Lactobacillus Strains Isolated from Sorghum-Based Traditional Fermented Food

Rao

Chennappa

Suraj

et al. 2015

Probiotics & Antimicro. Prot.

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Sorghum-based traditional fermented food was screened for potential probiotic lactic acid bacteria. The isolates were identified by biochemical, physiological and genetic methods. Species identification was done by 16s rRNA sequence analysis. The functional probiotic potential of the two Lactobacillus species viz., Lactobacillus plantarum (Lact. plantarum) and Lactobacillus pentosus (Lact. pentosus) was assessed by different standard parameters. The strains were tolerant to pH 2 for 1 h and resistant to methicillin, kanamycin, vancomycin and norfloxacin. Two (Lact. plantarum COORG-3 and Lact. pentosus COORG-8) out of eight isolates recorded the cell surface hydrophobicity to be 59.12 and 64.06%, respectively. All the strains showed tolerance to artificial duodenum juice (pH 2) for 3 h, positive for bile salt hydrolase test and negative for haemolytic test. The neutralized cell-free supernatant of the strains Lact. pentosus COORG-4, Lact. plantarum COORG-1, Lact. plantarum COORG-7, Lact. pentosus COORG-8 and Lact. plantarum COORG-3 showed good antibiofilm activity. Lact. pentosus COORG-8 exhibited 74% activity against Pseudomonas aeruginosa-MTCC 7903 and Lact. plantarum COORG-7 showed 68% inhibition of biofilm against Klebsiella pneumonia MTCC 7407. Three (Lact. plantarum COORG-7, Lact. pentosus COORG-5 and Lact. pentosus COORG 8) out of eight isolates exhibited a good antimicrobial activity against Listeria monocytogenes and five isolates (Lact. pentosus COORG 2, Lact. plantarum COORG 1, Lact. plantarum COORG 4, Lact. pentosus COORG 3 and Lact. plantarum COORG 6) are active against Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Enterobacter aerogenes, Klebsiella pneumonia, Enterococcus faecalis. The study also evaluated the cholesterol lowering property of the Lactobacillus strains using hen egg yolk as the cholesterol source. The cholesterol in hen egg yolk was assimilated by 74.12 and 68.26% by Lact. plantarum COORG 4 and Lact. pentosus COORG 7, respectively. The results of the present study suggest that the Lactobacillus strains isolated and characterized from sorghum-based fermented product may be used as probiotic strains for therapeutic applications.

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Antimicrobial Activity of a Bacteriocin Produced by Lactobacillus Plantarum 29V and Strain′S Viability in Palm Kernel Oil

Cited by 12 publications

References 19 publications

Probiotic Properties of Lactic Acid Bacteria Isolated From Egyptian Salted Food

Probiotic Properties of Lactic Acid Bacteria Isolated From Egyptian Salted Food

Cytotoxicity and antibacterial activity of gold-supported cerium oxide nanoparticles

Probiotic Potential of Lactobacillus Strains Isolated from Sorghum-Based Traditional Fermented Food

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