Antimicrobial Activity and Cell Cytotoxicity of Korean Plum-yem Extract

조철희,; 유귀재,; 김소영,; 이건순,; 김진현,; 현정오,; 채희정,

doi:10.3839/jabc.2009.009

Cited by 2 publications

(2 citation statements)

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“…This inhibition of microorganisms by phenolic compounds may be due to iron deprivation or hydrogen bonding with vital proteins such as microbial enzymes or other interactions to inactivate microbial adhesins, cell envelope transport proteins, and/or non-specific interactions with carbohydrates, among other possible effects [ 19 , 20 ]. Earlier, Cho et al [ 21 ] reported an inhibitory effect of Korean plum yew ( C. koreana ) extract on Gram-positive bacteria, Gram-negative bacteria, yeasts, and molds. Similarly, Watanabe and Fukao [ 22 ] reported that an extract of unripe Japanese plum yew fruit ( C. harringtonia ) possessed, among 101 edible plants, the highest inhibitory effect against Bacillus cereus and Leuconostoc mesenteroides .…”

Section: Discussionmentioning

confidence: 99%

Antioxidant, antibacterial, cytotoxic, and apoptotic activity of stem bark extracts of Cephalotaxus griffithii Hook. f

Moirangthem

Talukdar

Kasoju

et al. 2012

BMC Complement Altern Med

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BackgroundCephalotaxus spp. are known to possess various therapeutic potentials. Cephalotaxus griffithii, however, has not been evaluated for its biological potential. The reason may be the remoteness and inaccessibility of the habitat where it is distributed. The main aim of this study was to: (1) evaluate multiple biological potentials of stem bark of C. griffithii, and (2) identify solvent extract of stem bark of C. griffithii to find the one with the highest specific biological activity.MethodsDried powder of stem bark of C. griffithii was exhaustively extracted serially by soaking in petroleum ether, acetone and methanol to fractionate the chemical constituents into individual fractions or extracts. The extracts were tested for total phenolic and flavonoid content, antioxidant (DPPH radical scavenging, superoxide radical scavenging, and reducing power models), antibacterial (disc diffusion assay on six bacterial strains), cytotoxic (MTT assay on HeLa cells), and apoptotic activity (fluorescence microscopy, DNA fragmentation assay, and flow cytometry on HeLa cells).ResultsAmong the three extracts of stem bark of C. griffithii, the acetone extract contained the highest amount of total phenolics and flavonoids and showed maximum antioxidant, antibacterial, cytotoxic (IC50 of 35.5 ± 0.6 μg/ml; P < 0.05), and apoptotic (46.3 ± 3.6% sub-G0/G1 population; P < 0.05) activity, followed by the methanol and petroleum ether extracts. However, there was no significant difference observed in IC50 values (DPPH scavenging assay) of the acetone and methanol extracts and the positive control (ascorbic acid). In contrast, superoxide radical scavenging assay-based antioxidant activity (IC50) of the acetone and methanol extracts was significantly lower than the positive control (P < 0.05). Correlation analysis suggested that phenolic and flavonoid content present in stem bark of C. griffithii extracts was responsible for the high antioxidant, cytotoxic, and apoptotic activity (P < 0.05).ConclusionsStem bark of C. griffithii has multiple biological effects. These results call for further chemical characterization of acetone extract of stem bark of C. griffithii for specific bioactivity.

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Section: Discussionmentioning

confidence: 99%

Antioxidant, antibacterial, cytotoxic, and apoptotic activity of stem bark extracts of Cephalotaxus griffithii Hook. f

Moirangthem

Talukdar

Kasoju

et al. 2012

BMC Complement Altern Med

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“…우리나라에서도 천연물의 생리활성 성분을 이용한 다양한 분야에 대한 연구가 진행되고 있으며 특히 질병에 대한 약물 의 개발에 많은 연구가 이루어지고 있다 [2,[8][9][10][11]19]. 그리고 약 물의 개발뿐 아니라 화장품소재, 식품첨가물과 염료 등 일상 생활에서 많이 사용되는 합성물질과 사료 중 항생제를 대체할 수 있는 물질의 개발에도 많은 연구가 진행 중이다 [3,4,6,12,23,24].…”

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Antibacterial Activity of Oriental Medicinal Herb Extracts against Skin Pathogens

2010

Journal of Life Science

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The antibacterial activity of methanol extracts from 70 kinds of oriental medicinal herbs on four strains of Staphylococcus aureus and S. epidermidis was investigated. The results showed that C. japonica, C. sappan, R. javanica, R. tanguticum, and S. miltiorrhiza had an antibacterial activity on all the strains used. Among these, C. japonica and R. javanica, which showed excellent antibacterial activity, were extracted with water, ethanol, methanol, and ethyl acetate for further study of antibacterial activities. The results showed that the boiled water extract of C. japonica had the best antibacterial activity. Assuming that the antibacterial activity of C. japonica originated from berberine, which has been reported many times, the berberine content of the boiled water extract of C. japonica was analyzed and the result was 13.88%. To compare the antibacterial activity of berberine with that of other antibiotics, berberine chloride and three other kinds of antibiotics were investigated, which showed that berberine chloride had an antibacterial activity on KCCM 35494 S. epidermidis only when the concentration was higher than 600 mg/l, and it did not show antibacterial activity in the other strains. Based on these results, it was concluded that the antibacterial activity of the boiled water extract of C. japonica on the strains used in this study originated from high concentration of berberine or substances other than berberine. Therefore, identification of the substance will be necessary.

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Antimicrobial Activity and Cell Cytotoxicity of Korean Plum-yem Extract

Cited by 2 publications

References 7 publications

Antioxidant, antibacterial, cytotoxic, and apoptotic activity of stem bark extracts of Cephalotaxus griffithii Hook. f

Antioxidant, antibacterial, cytotoxic, and apoptotic activity of stem bark extracts of Cephalotaxus griffithii Hook. f

Antibacterial Activity of Oriental Medicinal Herb Extracts against Skin Pathogens

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