Antimicrobial activity and biofilm formation inhibition of green tea polyphenols on human teeth

Cho, Yun-Seok; Oh, Jay Jooyoung; Oh, Keun-Yeob

doi:10.1007/s12257-009-0195-8

Cited by 29 publications

(16 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The activity observed was most likely due to the polyphenols such as catechin found in the green tea leaf, which seem to affect the double layer of the cell membrane that is critical for bacterial resistance to antimicrobials as well as other cell functions 9 . Our results corroborate evidence demonstrating in vitro antimicrobial activity of green tea on various bacteria 15,16 . Furthermore, the consumption of green tea can provide clinical benefits in the treatment of gingivitis 13,20 and periodontitis with greater reductions in probing depth and gains in clinical attachment 9 .…”

Section: Discussionsupporting

confidence: 90%

“…Some evidence suggests that green tea extract has antimicrobial activity against anaerobic bacteria that are typically associated with periodontal diseases 10 and against cariogenic bacteria 14 . In addition, green tea inhibits the growth and adherence of Porphyromonas gingivalis 15 , Prevottela intermedia 12 and several other bacteria 16 . This evidence suggests that the natural product may inhibit the adhesion phase of biofilm formation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Oxygen tension during biofilm growth influences the efficacy antimicrobial agents

Antoniazzi

Trojahn

Casarin³

et al. 2016

Rev. odontol. UNESP

View full text Add to dashboard Cite

ResumoObjetivo: Comparar a eficácia antimicrobiana de clorexidina 0,12% (CHX) e solução de chá verde (CV) (Camellia sinensis) em biofilmes formados em diferentes tensões de oxigênio em um modelo in situ. Método: Vinte e cinco estudantes de odontologia foram elegíveis. Um dispositivo com espécimes de esmalte (EE) padronizados, voltado para o palato e para vestibular foram inseridos nos voluntários por 7 dias. Durante os primeiros quatro dias nenhum agente foi aplicado. A partir do quinto dia, ambos os agentes foram aplicados nos EEs no grupo teste e nenhum agente foi aplicado no grupo EE controle. Após 7 dias, os fragmentos EEs foram removidos dos dispositivos, sonicados, plaqueados em ágar, e incubado durante 24 h a 37 °C para determinar unidades formadoras de colonias (UFCs). Resultado: CHX teve significativamente melhor eficácia em comparação com CV nos EEs para vestibular (1.330 vs. 2.170 UFC/mL) e EEs voltados para o palatal (2.250 vs. 2.520 UFC/mL). Comparações intragrupos mostraram maior eficácia em EEs vestibulares do que as EEs voltadas para o palato (1.330 vs. 2.250 UFC/mL para CHX e 2170 vs, 2520 UFC/mL para CV) em ambas as soluções. Análise dos controles, mostrou a formação de biofilme significativamente maior em comparação com EEs palatinas e bucais. Conclusão: CHX tem uma eficácia maior do que o chá verde em biofilmes formados por 4 dias. A eficácia dos agentes foi reduzida para os biofilmes em ambiente com baixa tensão de oxigênio. A tensão de oxigênio parece influenciar na eficácia dos agentes testados.Descritores: Camellia sinensis; clorexidina; placa dentária. AbstractObjective: To compare the antimicrobial efficacy of a 0.12% chlorhexidine (CHX) and herbal green tea (Camellia sinensis) solution on established biofilms formed at different oxygen tensions in an in situ model. Method: Twenty-five dental students were eligible for the study. In situ devices with standardized enamel specimens (ES) facing the palatal and buccal sides were inserted in the mouths of volunteers for a 7 day period. No agent was applied during the first four days. From the fifth day onward, both agents were applied to the test ES group and no agent was applied to the control ES group. After 7 days the ES fragments were removed from the devices, sonicated, plated on agar, and incubated for 24 h at 37 °C to determine and quantify the colony forming units (CFUs). Result: CHX had significantly higher efficacy compared to green tea on the buccal (1330 vs. 2170 CFU/µL) and palatal (2250 vs. 2520 CFU/µL) ES. In addition, intragroup comparisons showed significantly higher efficacy in buccal ES over palatal ES (1330 vs. 2250 CFU/µL for CHX and 2170 vs, 2520 CFU/µL for CV) for both solutions. Analysis of the ES controls showed significantly higher biofilm formation in palatal ES compared to buccal ES. Conclusion: CHX has higher efficacy than green tea on 4-day biofilms. The efficacy of both agents was reduced for biofilms grown in a low oxygen tension environment. Therefore, the oxygen tension environment seems to influence the eff...

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Oxygen tension during biofilm growth influences the efficacy antimicrobial agents

Antoniazzi

Trojahn

Casarin³

et al. 2016

Rev. odontol. UNESP

View full text Add to dashboard Cite

show abstract

“…For instance, Lee et al reported that the simple dihydrochalcone phloretin (2=,4,4=,6=-tetrahydroxydihydrochalcone) inhibits biofilm formation of the pathogenic E. coli strain O157:H7. Phloretin, however, does not promote the growth of planktonic E. coli O157:H7 or induce its functional differentiation to produce enterotoxin (42). Conversely, monocultured B. unamae CK43B formed a viable biofilm in MW medium in our experiments only when indole and gallic acid were both present.…”

Section: Figcontrasting

confidence: 57%

“…Generally, polyphenols inhibit biofilm formation of eubacteria (39)(40)(41)(42). For instance, Lee et al reported that the simple dihydrochalcone phloretin (2=,4,4=,6=-tetrahydroxydihydrochalcone) inhibits biofilm formation of the pathogenic E. coli strain O157:H7.…”

Section: Figmentioning

confidence: 99%

Induction of Biofilm Formation in the Betaproteobacterium Burkholderia unamae CK43B Exposed to Exogenous Indole and Gallic Acid

Kim

Sitepu

Hashidoko

2013

Appl Environ Microbiol

View full text Add to dashboard Cite

bBurkholderia unamae CK43B, a member of the Betaproteobacteria that was isolated from the rhizosphere of a Shorea balangeran sapling in a tropical peat swamp forest, produces neither indole nor extracellular polymeric substances associated with biofilm formation. When cultured in a modified Winogradsky's medium supplemented with up to 1.7 mM indole, B. unamae CK43B maintains its planktonic state by cell swelling and effectively degrades exogenous indole. However, in medium supplemented with 1.7 mM exogenous indole and 1.0 mM gallic acid, B. unamae CK43B produced extracellular polymeric substances and formed a biofilm. The concentration indicated above of gallic acid alone had no effect on either the growth or the differentiation of B. unamae CK43B cells above a certain concentration threshold, whereas it inhibited indole degradation by B. unamae CK43B to 3-hydroxyindoxyl. In addition, coculture of B. unamae CK43B with indole-producing Escherichia coli in nutrient-rich Luria-Bertani medium supplemented with 1.0 mM gallic acid led to the formation of mixed cell aggregates. The viability and active growth of B. unamae CK43B cells in a coculture system with Escherichia coli were evidenced by fluorescence in situ hybridization. Our data thus suggest that indole facilitates intergenus communication between indole-producing gammaproteobacteria and some indole-degrading bacteria, particularly in gallic acid-rich environments.

show abstract

“…It is well established that membrane fusion by polyethylene glycol, calcium ion, or virions has consistently resulted in the leakage of intramembranous materials and aggregation (Ikigai et al, 1993). SEM analysis of oral microorganisms treated with tea polyphenol showed major structural cell surface changes and irregular forms, with aggregates among cells (Cho et al, 2010). It was reported that damage to bacterial cells might manifest in a few ways, one of which was loss of membrane integrity resulting in leakage of essential intracellular constituents such as inorganic phosphate and proteins.…”

Section: Discussionmentioning

confidence: 99%

Effect of Roselle Calyx Extract on Gingipain Activity, Production of Inflammatory Cytokines, and Oral Bacterial Morphology

Sulistyani¹,

Fujita²,

Nakazawa³

2016

J microb biotech food sci

View full text Add to dashboard Cite

This study to investigate the effect of roselle calyx extract on gingipain activity, production of inflammatory cytokines, and oral bacterial morphology. The inhibitory effect of roselle calyx extract (RCE) on Arg- (Rgp) and Lys-gingipain (Kgp) was evaluated using synthetic substrate colorimetric assay with a 96-well microtiter plate. RCE with various concentrations was tested for determination of interleukin (IL)-6 and IL-8 produced from KB cells stimulated with heat-inactivated P. gingivalis. The production of IL-6 and IL-8 was quantified using an ELISA kit. The morphological alterations in cells of S. mutans and P. gingivalis after treatment with RCE were studied using scanning electron microscopy (SEM). RCE could inhibit both Rgp (90%) and Kgp (70%) activities significantly at concentrations lower than the minimum inhibitory concentration (MIC) (2.2 mg/mL). Furthermore, the production of IL-6 and IL-8 from KB cells stimulated with P. gingivalis was significantly inhibited by RCE at 6 h after exposure, in a dose-dependent manner. After treatment with RCE, the morphological alterations were observed in cells of S. mutans and P. gingivalis by using SEM. The ability of RCE to inhibit gingipain activity and production of inflammatory cytokines indicates that RCE could be considered for prevention and clinical treatment for periodontitis.

show abstract

Antimicrobial activity and biofilm formation inhibition of green tea polyphenols on human teeth

Cited by 29 publications

References 28 publications

Oxygen tension during biofilm growth influences the efficacy antimicrobial agents

Oxygen tension during biofilm growth influences the efficacy antimicrobial agents

Induction of Biofilm Formation in the Betaproteobacterium Burkholderia unamae CK43B Exposed to Exogenous Indole and Gallic Acid

Effect of Roselle Calyx Extract on Gingipain Activity, Production of Inflammatory Cytokines, and Oral Bacterial Morphology

Contact Info

Product

Resources

About