Antimicrobial activity and biocompatibility of the mixture of mineral trioxide aggregate and nitric oxide-releasing compound

Abstract

Background/purpose Antimicrobial activity and biocompatibility of root canal sealer are related to the success of endodontic treatments. This study investigated the efficacy of mixture of mineral trioxide aggregate (MTA) and a NO-releasing compound for the antimicrobial activity, biocompatibility, and physical properties. Materials and methods MTA was mixed with diethylenetriamine-NO (MTA-NO), and the extracts from MTA and the MTA-NO mixture before and after setting was obtained were investigated the antimicrobial activity against Enterococcus faecalis and Porphyromonas endodontalis . After setting MTA and MTA-NO, pulp cell was incubated in the presence of MTA and MTA-NO disk using Transwell® cell culture insert, and the proliferation assay and mineralization-stimulated factors of the cells were analyzed by MTT assay and real-time RT-PCR, respectively. The physical properties of MTA and the MTA-NO mixture, such as surface hardness and flowability was also analyzed. Results The MTA-NO mixture showed stronger antimicrobial activity against E. faecalis and P. endodontalis than that by MTA. Both MTA and MTA-NO mixture increase the ratio of cell proliferation and induced the expression of alkaline phosphatase, collagen type I, osteocalcin, and osteopontin. Moreover, the induction of gene expression by MTA-NO mixture was higher than that by MTA alone. No significant difference was observed for surface hardness and flowability between MTA and MTA-NO mixture. Conclusion The addition of a NO-releasing compound to the endodontic treatment using MTA root canal sealer might reduce the risk of bacterial infection and help to regenerate the dental pulp tissue.

Antimicrobial Action, Genotoxicity, and Morphological Analysis of Three Calcium Silicate-Based Cements

“…In a more recent study, it was found that MTA HP was not effective over P. gingivalis [ 18 ], and these outcomes agree with the reported results in the present study, in which MTA HP was not effective over none of the tested anaerobic bacteria except of P. micra . Besides, there is only one study in the literature that evaluated the effect of MTA over P. endodontalis in which the effectivity of MTA was improved with the addition of nitric oxide [ 19 ]. According to the same study, the antimicrobial action of the CSC is affected by its setting [ 19 ].…”

“…Besides, there is only one study in the literature that evaluated the effect of MTA over P. endodontalis in which the effectivity of MTA was improved with the addition of nitric oxide [ 19 ]. According to the same study, the antimicrobial action of the CSC is affected by its setting [ 19 ]. In the present study, only 5MO was effective over P. endodontalis.…”

Modified Mineral Trioxide Aggregate—A Versatile Dental Material: An Insight on Applications and Newer Advancements

“…Perforations in the roots are one of the reasons for failure in endodontics, and if they are not detected and corrected promptly, they can result in the loss of the affected teeth. MTA has several desirable properties for endodontic repair, including strong sealing ability, biocompatibility, antibacterial properties, and hydrophilic behavior ( Shin et al, 2021 ).…”

“…Various studies have linked five– seven bacterial species to root canal infection, with Porphyromonas gingivalis , Porphyromonas endodontalis , and Enterococcus faecalis being the most often isolated bacterial species from pulpitis. Apical periodontitis is linked to Enterococcus faecalis in RC-treated teeth, whereas Porphyromonas endodontalis and Porphyromonas gingivalis are related to the infection of the root canal (RC) ( Shin et al, 2021 ). Because the root canal system is anatomically complex, the complete elimination of pathogenic microorganisms in the root canal is challenging.…”

Adhesive Ability of Different Oral Pathogens to Various Dental Materials: An in Vitro Study

“…Many studies are available on the antibacterial properties of MTA. Several investigations have demonstrated limited antibacterial properties of MTA against some microorganisms [29][30][31][32] . A study on facultative and obligate anaerobes showed that [30] MTA had antibacterial effects on some facultative anaerobes, with little impact on strict obligate anaerobes.…”