Antimalarial activities of Tithonia diversifolia (Asteraceae) and Crossopteryx febrifuga (Rubiaceae) on mice in vivo

Elufioye, Taiwo O.; Agbedahunsi, Joseph M.

doi:10.1016/j.jep.2004.01.009

Cited by 90 publications

(55 citation statements)

References 9 publications

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“…Pharmacological studies of Tithonia diversifolia showed that it has anti-diabetic, 6,7) anti-malarial, 8) anti-inflammatory, 9) analgesic, 9) and cancer chemopreventive 10) activity, some of which account for the folkloric claims of this medicinal plant. Several sesquiterpenoids were isolated from T. diversifolia.…”

Section: -5)mentioning

confidence: 99%

Sesquiterpenoids and Flavonoids from the Aerial Parts of Tithonia diversifolia and Their Cytotoxic Activity

et al. 2007

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Tithonia diversifolia (HEMSL.) A. GRAY (Compositae) is native to Mexico and also grows in parts of Africa, Australia, Asia, and other countries of North America, and is commonly called Mexican sunflower or tree marigold.1) An extract of T. diversifolia has been traditionally used for the treatment of diabetes, diarrhea, menstrual pain, malaria, hematomas, hepatitis, hepatomas, and wound healing. 2-5)Pharmacological studies of Tithonia diversifolia showed that it has anti-diabetic, 6,7) anti-malarial, 8) anti-inflammatory, 9) analgesic, 9) and cancer chemopreventive 10) activity, some of which account for the folkloric claims of this medicinal plant. Several sesquiterpenoids were isolated from T. diversifolia.1,10,11) As part of our systematic survey of bioactive compounds from higher plants, we have now conducted a detailed phytochemical screening of the 80% EtOH extract of T. diversifolia using a cytotoxicity-guided fractionation method, which has resulted in the isolation of twelve sesquiterpenoids (1-12), including three new ones (4, 10, 12), and three known flavonoids (13)(14)(15). This paper deals with the structural identification and determination of the sesquiterpenoids and flavonoids, and with their cytotoxic activity. Results and DiscussionThe dried aerial parts of T. diversifolia were extracted with hot 80% EtOH. The concentrated 80% EtOH extract, which showed cytotoxic activity against HL-60 cells with an IC 50 value of 4.10 mg/ml, was passed through a porous-polymer polystyrene resin (Diaion HP-20) column, and successively eluted with 30% MeOH, EtOH, and EtOAc. The EtOH eluate fraction exhibited cytotoxic activity against HL-60 cells (IC 50 1.03 mg/ml), and was repeatedly subjected to column chromatography on silica gel, octadecylsilanized (ODS) silica gel, Sephadex LH-20, and reversed phase HPLC, giving compounds 1 (8.4 mg), 2 (11.3 mg), 3 (118 mg), 4 (744 mg), 5 (112 mg), 6 (8.7 mg), 7 (67.9 mg), 8 (90.7 mg), 9 (245 mg), 10 (14.0 mg), 11 (24.2 mg), 12 (19.0 mg), 13 (18.8 mg), 14 (60.8 mg), and 15 (6.6 mg).Compounds 1-3, 5-9, 11, and 13-15 were identified as tirotundin (1), 12) tirotundin 3-O-methyl ether (2), 13) tagitinin A (3), 14) tagitinin C (5), 12) deacetylviguiestin (6), 15) tagitinin F (7), 12) 1b-methoxydiversifolin (8), 1) 1b-methoxydiversifolin 3-O-methyl ether (9), 1) 4b,10a-dihydroxy-3-oxo-8b-isobutyroyloxyguaia-11(13)-en-6,12-olide (11), 16) luteolin (13), 16) nepetin (14), 16) and hispidulin (15) Hachioji, Tokyo 192-0392, Japan: b Hiro International Co., Ltd.; 2-16-5 Shibuya, Shibuya-ku, Tokyo 150-0002, Japan: and c Division of Molecular Pharmacology, Cancer Chemotherapy Center, Japanese Foundation for Cancer Research; 3-10-6 Ariake, Koto-ku, Tokyo 135-8550, Japan. Received April 26, 2007; accepted May 15, 2007 Cytotoxicity-guided fractionation of the 80% EtOH extract of Tithonia diversifolia has resulted in the isolation of twelve sesquiterpenoids (1-12), including three new ones (4, 10, 12), and three known flavonoids (13-15). The structures of the new compounds were deter...

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Section: -5)mentioning

confidence: 99%

Sesquiterpenoids and Flavonoids from the Aerial Parts of Tithonia diversifolia and Their Cytotoxic Activity

et al. 2007

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“…We employed a 4-day blood schizonticidal test modified from previous studies (Peters et al, 1975;Elufioye & Agbedahunsi, 2004). Each mouse received standard inoculum of 1 × 10 7 Plasmodium yoelii-infected erythrocytes intraperitoneally on day 1.…”

Section: Methods For In Vivo Suppression Of Parasitemiamentioning

confidence: 99%

In vivoantimalarial activities of glycoalkaloids isolated from Solanaceae plants

Chen¹,

Li²,

Sun³

et al. 2010

Pharmaceutical Biology

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“…TG ve KG gruplarına tedavi intragastrik lavaj yoluyla 50 mg/kg dozunda oral olarak uygulanmıştır. TAKG'na ise aynı miktarda serum fizyolojik verilmiştir (23,24,25).…”

Section: Sıtmada In Vivo Modelunclassified

“…Klorokin gruplarında tedavi dozu olarak günde tek doz 50 mg/ kg olarak oral yolla 3 gün boyunca farelere klorokin verilen başka araştırmacıların yaptığı çalışmalarda da benzer sonuçların alındığı rapor edilmiştir (24,34).…”

Section: Sıtmada In Vivo Modelunclassified

In vivo and in vitro Models for Scanning Drug Substances in Malaria: Prestudy

Özbilgin

Çavuş²,

Nuraydın³

et al. 2017

TurkiyeParazitolDerg

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Sıtmada Etken Madde Taramalarında in vivo ve in vitro Modeller: Pilot ÇalışmaIn vivo and in vitro Models for Scanning Drug Substances in Malaria: Prestudy ÖZ Amaç: Dünya Sağlık Örgütü Plasmodium türlerinin "artemisinin" içeren ilaçlara da dirençli suşlarının ortaya çıkmasıyla tüm ülkeleri "Bir Sonraki Artemisinin Avı'' sloganıyla bitkisel kaynaklı antimalaryal etken maddeleri araştırmaya teşvik etmektedir. Çalışacak genç araştırmacılara rehber olacak şekilde en temel ve basit olarak sıtmanın in vivo ve in vitro modellerinin kurularak ilaç araştırmalarında ve etken madde taramalarında yardımcı olması amacıyla planlanmıştır. Yöntemler: İn vitro çalışmamızda sıvı azot tankından çıkarılarak uygun koşullarda çözdürülen Plasmodium berghei genç trofozoitleri 0.1, 0.4, 0.8, 1.6, 6.4, 12.8 µg/mL konsantrasyonunda klorokin ve tetrasiklin ilaçları ile 24 saat +37°C'de jar içerisinde çalkalamalı etüvde inkübe edilerek takip edilmiştir. İn vivo çalışmalarda Tetrasiklin grubu (TG) ve Klorokin grubuna (KG) intragastrik lavaj yoluyla 50 mg/kg dozunda tetrasiklin ve klorokin oral yolla, tedavi almamış kontrol grubuna (TAKG) aynı miktarda ve yolla serum fizyolojik verilmiştir. Farelerdeki parazitemi 24 gün boyunca takip edilmiştir. Bulgular: İn vitro ilaç çalışmamızda klorokinin 0,8 μg/mL dozda parazitemiyi baskıladığı görülürken, tetrasiklinin 1,6 μg/mL dozda parazitemiyi baskıladığı görülmüştür. İn vivo ilaç çalışmamızda KG grubunda farelerde parazitemi yok olurken TG grubundaki farelerin hepsi 24. günde, TAKG grubundaki farelerin hepsi 12. günde ölmüştür. Sonuç: Çalışmamız, klorokin temini geciktiğinde tetrasiklin tedavisi verilmesi ile paraziteminin ilerlemesinin geciktirilebileceği bu sürede klorokinin temin edilmesiyle ve tedaviye klorokin ile devam edilmesi durumunda hastanın ex olmasının önlenebileceğini düşündürmüştür. Anahtar Kelimeler: Plasmodium, in vitro, in vivo, Manisa, Türkiye Geliş Tarihi: 25.04.2017 Kabul Tarihi: 03.08.2017ABSTRACT Objective: The Wolrd Health Organization (WHO) encourages all countries to investigate antimalarial drug substances derived from herbal sources with the slogan "Hunt of the Next Artemisinin" due to the emergence of resistant strains of Plasmodium species to artemisinin. In the broad and simple sense, it was planned to help guide the young researchers set in-vitro and in-vivo models of malaria in order to be used in drug research and active ingredient studies. Methods: In-vitro study, young Plasmodium berghei trophozoites were removed from the liquid nitrogen tank and resuspended in appropriate conditions, followed by incubation with chloroquine and tetracycline at concentrations of 0.1, 0.4, 0.8, 1.6, 6.4, 12.8 μg/mL for 24 hours at +37°C in a shaking incubator. In-vivo studies, Tetracycline group (TG) and Chloroquine group (KG) were administered 50 mg/kg of tetracycline and chloroquine by intragastric lavage and untreated control group (TACG) were administered the same amount of saline via the same route. The suppression of parasitemia in mice was followed for 24 days.Results: In ...

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Antimalarial activities of Tithonia diversifolia (Asteraceae) and Crossopteryx febrifuga (Rubiaceae) on mice in vivo

Cited by 90 publications

References 9 publications

Sesquiterpenoids and Flavonoids from the Aerial Parts of Tithonia diversifolia and Their Cytotoxic Activity

Sesquiterpenoids and Flavonoids from the Aerial Parts of Tithonia diversifolia and Their Cytotoxic Activity

In vivoantimalarial activities of glycoalkaloids isolated from Solanaceae plants

In vivo and in vitro Models for Scanning Drug Substances in Malaria: Prestudy

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