Sıtmada Etken Madde Taramalarında in vivo ve in vitro Modeller: Pilot ÇalışmaIn vivo and in vitro Models for Scanning Drug Substances in Malaria: Prestudy ÖZ Amaç: Dünya Sağlık Örgütü Plasmodium türlerinin "artemisinin" içeren ilaçlara da dirençli suşlarının ortaya çıkmasıyla tüm ülkeleri "Bir Sonraki Artemisinin Avı'' sloganıyla bitkisel kaynaklı antimalaryal etken maddeleri araştırmaya teşvik etmektedir. Çalışacak genç araştırmacılara rehber olacak şekilde en temel ve basit olarak sıtmanın in vivo ve in vitro modellerinin kurularak ilaç araştırmalarında ve etken madde taramalarında yardımcı olması amacıyla planlanmıştır. Yöntemler: İn vitro çalışmamızda sıvı azot tankından çıkarılarak uygun koşullarda çözdürülen Plasmodium berghei genç trofozoitleri 0.1, 0.4, 0.8, 1.6, 6.4, 12.8 µg/mL konsantrasyonunda klorokin ve tetrasiklin ilaçları ile 24 saat +37°C'de jar içerisinde çalkalamalı etüvde inkübe edilerek takip edilmiştir. İn vivo çalışmalarda Tetrasiklin grubu (TG) ve Klorokin grubuna (KG) intragastrik lavaj yoluyla 50 mg/kg dozunda tetrasiklin ve klorokin oral yolla, tedavi almamış kontrol grubuna (TAKG) aynı miktarda ve yolla serum fizyolojik verilmiştir. Farelerdeki parazitemi 24 gün boyunca takip edilmiştir. Bulgular: İn vitro ilaç çalışmamızda klorokinin 0,8 μg/mL dozda parazitemiyi baskıladığı görülürken, tetrasiklinin 1,6 μg/mL dozda parazitemiyi baskıladığı görülmüştür. İn vivo ilaç çalışmamızda KG grubunda farelerde parazitemi yok olurken TG grubundaki farelerin hepsi 24. günde, TAKG grubundaki farelerin hepsi 12. günde ölmüştür. Sonuç: Çalışmamız, klorokin temini geciktiğinde tetrasiklin tedavisi verilmesi ile paraziteminin ilerlemesinin geciktirilebileceği bu sürede klorokinin temin edilmesiyle ve tedaviye klorokin ile devam edilmesi durumunda hastanın ex olmasının önlenebileceğini düşündürmüştür. Anahtar Kelimeler: Plasmodium, in vitro, in vivo, Manisa, Türkiye Geliş Tarihi: 25.04.2017 Kabul Tarihi: 03.08.2017ABSTRACT Objective: The Wolrd Health Organization (WHO) encourages all countries to investigate antimalarial drug substances derived from herbal sources with the slogan "Hunt of the Next Artemisinin" due to the emergence of resistant strains of Plasmodium species to artemisinin. In the broad and simple sense, it was planned to help guide the young researchers set in-vitro and in-vivo models of malaria in order to be used in drug research and active ingredient studies. Methods: In-vitro study, young Plasmodium berghei trophozoites were removed from the liquid nitrogen tank and resuspended in appropriate conditions, followed by incubation with chloroquine and tetracycline at concentrations of 0.1, 0.4, 0.8, 1.6, 6.4, 12.8 μg/mL for 24 hours at +37°C in a shaking incubator. In-vivo studies, Tetracycline group (TG) and Chloroquine group (KG) were administered 50 mg/kg of tetracycline and chloroquine by intragastric lavage and untreated control group (TACG) were administered the same amount of saline via the same route. The suppression of parasitemia in mice was followed for 24 days.Results: In ...
Objective: Human African trypanosomiasis, also known as sleeping sickness, is a parasitic disease in which Glossina is transmitted by human intervention and Trypanosoma b. rhodosiense and Trypanosoma b. gambiense are the causative agents Production of parasites in axenic cultures provides great advantage in parasite biochemistry, immunological, physiological and molecular studies. In this study, it is aimed to determine the medium which will produce in vigorous amount of Trypanosoma b. rhodosiense and Trypanasoma cruzi and to establish a new medium. Methods: In this study, Trypanosoma b. rhodosiense and Trypanasoma cruzi strains stored in Manisa Celal Bayar University Parasite Bank will be removed from liquid nitrogen tank under suitable conditions, planted in Medium I, Medium II, Medium III and newly developed medium. Reproductive densities of the media will be statistically analyzed on Thoma lamina depending on the time, using the Sidak's multiplequality test. Results: As a result of this study, it has been concluded that the best medium, to produce abundantly Trypanosoma b. rhodosiense and Trypanasoma cruzi strains, to be used in diagnosis and active substance screenings, molecular studies, metabolic analyzes and drug studies is the medium IV. Conclusion: This study is one of the first studies related to the production of Trypanosoma species in Turkey and planned to provide a basis for the studies of African sleeping disease, Chagas disease and their agents.
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