Antileukemic Effect of Tualang Honey on Acute and Chronic Leukemia Cell Lines

Man, Nik Muhd Khuzaimi Nik; Hassan, Rosline; Ang, Cheng Yong; Abdullah, Abu Dzarr; Radzi, Muhammad; Sulaiman, Siti Amrah

doi:10.1155/2015/307094

Cited by 21 publications

(13 citation statements)

References 12 publications

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“…Therefore, there has been an increase in the number of studies investigating the potential role of honey in the prevention of cancer development and progression. These studies have been mainly conducted on certain cancer cell lines such as liver , prostate , endometrial , leukemia , colorectal , and breast . The findings suggest that honey generally exerts antiproliferative and antiapoptotic effects on cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Anatolian honey is not only sweet but can also protect from breast cancer: Elixir for women from artemis to present

et al. 2017

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Natural products with bioactive components are widely studied on various cancer cell lines for their possible cytotoxic effects, recently. Among these products, honey stands out as a valuable bee product containing many active phenolic compounds and flavonoids. Numerous types of multifloral honey and honeydew honey are produced in Turkey owing to its abundant vegetation. Therefore, in this study, we investigated the cytotoxic effects of particular tree-originated honeys from chestnut, cedar, pine, and multifloral honey on cell lines representing different types of the most common cancer of women, breast cancer, MCF7, SKBR3, and MDAMB-231, and fibrocystic breast epithelial cell line, MCF10A as a control. All honey samples were analyzed biochemically. The dose- (1, 2.5, 5, 7.5, and 10 µg/mL) and time (24th, 48th, and 72nd hours)-dependent effects of ethanol/water solutions of the honey samples were scrutinized. Cell viability/cytotoxicity was evaluated by the water soluble tetrazolium Salt-1 (WST-1) method. Apoptotic status was detected by Annexin V-PI assay using FACSCalibur. The statistical analysis was performed using GraphPad Prism 6 and the clustering data analysis with the R programming language. The biochemical analyses of the honey samples showed that the tree-originated honey samples contained more total phenolic compounds than the multifloral honey. Phenolic content of the honey types increases in order of multifloral, pine, cedar, and chestnut, respectively, which is compatible with their cytotoxic affectivity and dark color. In addition, the antioxidant capacity of the studied honey types was observed to increase in order of multifloral < pine < cedar ≅ chestnut. According to the WST-1 data, chestnut honey induced cytotoxicity over 50% on all the cell lines, including the control MCF10A cells, even with low doses (honey concentrations starting from 1 µg/mL) (P < 0.0001). Similarly, Cedar honey was observed to be the second most effective honey in this study. Cedar honey, with the dose of 1 µg/mL, was detected statistically highly significant on MCF10A, MCF7, and SKBR3. In contrast, pine honey showed dramatically significant cytotoxicity only on the MDAMB 231 cells with a 1 µg/mL dose at the same time point (P = 0.018). While pine honey caused an anticancer effect on the MCF-7 and SKBR3 cancer cell lines with a 2.5-5 µg/mL dose (P < 0.0001), like cedar and chestnut honeys, it increased the viability of the MCF10A control cells with the doses of 2.5-5 µg/mL. It only showed cytotoxicity with higher doses (10 µg/mL) on the MCF10A cell line (P < 0.0001). Moreover, we have observed that the multifloral and artificial honey samples were mostly ineffective or increased cell viability with the doses of 1-5 µg/mL. Apoptotic effects of the other honey samples on the MCF-7 cell line were found as chestnut> pine> cedar> multifloral in the Annexin V-propidium iodide (PI) analysis. Chestnut, cedar, and pine honey displayed a remarkably cytotoxic effect on breast cancer cell lines, MCF7, SKBR3, and even on the most agg...

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Section: Discussionmentioning

confidence: 99%

Anatolian honey is not only sweet but can also protect from breast cancer: Elixir for women from artemis to present

et al. 2017

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“…Most important bioactive compounds in honey are polyphenols. for example, Tualang honey inhibited proliferation of K562 and MV4-11 leukaemia cells (Nik Man et al, 2015). This Malaysian honey is collected from beehives in Tualang trees (Koompassia excelsa) and is rich in polyphenols that are also present in Zantaz honey, including syringic acid, p-coumaric acid, catechin, luteolin and apigenin (Kishore, Halim, Syazana, & Sirajudeen, 2011).…”

Section: Cellular Antiproliferative Activity Of Zantaz Honeymentioning

confidence: 99%

Physicochemical characteristics and antiproliferative and antioxidant activities of Moroccan Zantaz honey rich in methyl syringate

et al. 2021

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“…Honey possesses many beneficial properties such as neuroprotective, anti-oxidative, anti-inflammatory, antibacterial, wound healing, anticancer etc. [5,6,13,14]. Based on the current literature search, there has been very little research on the effect of honey on learning and memory on chronic cerebral hypoperfusion induced neurodegeneration in animal models.…”

Section: Discussionmentioning

confidence: 99%

“…Honey possesses numerous beneficial effects including antimicrobial, antiviral, antiparasitory, anti-inflammatory, antioxidant, antimutagenic, neuroprotective, antitumor, wound healing and cardio protective effects [5][6][7]. These activities are mainly due to the phenolic compounds such as flavonoid.…”

Section: Introductionmentioning

confidence: 99%

Improved spatial learning and memory performance following Tualang honey treatment during cerebral hypoperfusion-induced neurodegeneration

Saxena¹,

Phyu²,

Al-Ani³

et al. 2016

J Transl Sci

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Honey has shown potential therapeutic properties in traditional medicine. This study investigated the cognition enhancing potential of Tualang honey in chronic cerebral hypoperfusion-induced neurodegeneration by permanent bilateral common carotid arteries ligation (2 vessels occlusion-2VO) in rats. Previous studies have shown that rats subjected to 2VO (2 vessel occlusion) experienced cognitive deficits, oxidative stress and neuronal injury in the hippocampus of the brain. We studied the effect of Tualang honey on learning and memory using the Morris water maze (MWM) in rats. Rats were randomly divided into four groups (n=10); Sham control, untreated 2VO (2VO), honey treated 2VO (2VO+H) and honey treated group (H). Each group was again divided into two subgroups (n=5): one for long term memory assessment and another for short term memory and relearning tasks. After 10 weeks of treatment, all rat groups were tested for cognitive assessment by MWM (Morris water maze). 2VO+H rats had better spatial learning and memory performance than untreated 2VO rats in MWM tasks (p<0.05). However, there was no significant difference between Sham rats and H group rats (p>0.05). This study shows that Malaysian Tualang honey might have therapeutic potential for the treatment of chronic cerebral hypoperfusion related neurodegenerative disorders such as Alzheimer's disease (AD) in which cognitive impairment is profound.

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Antileukemic Effect of Tualang Honey on Acute and Chronic Leukemia Cell Lines

Cited by 21 publications

References 12 publications

Anatolian honey is not only sweet but can also protect from breast cancer: Elixir for women from artemis to present

Anatolian honey is not only sweet but can also protect from breast cancer: Elixir for women from artemis to present

Physicochemical characteristics and antiproliferative and antioxidant activities of Moroccan Zantaz honey rich in methyl syringate

Improved spatial learning and memory performance following Tualang honey treatment during cerebral hypoperfusion-induced neurodegeneration

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