Antileishmanial and antifungal activity of plants used in traditional medicine in Brazil

Braga, Fernanda Gambogi; Bouzada, Maria Lúcia M.; Fabri, Rodrigo Luiz; Matos, Magnum de Oliveira; Moreira, Francis O.; Scio, Elita; Coimbra, Elaine Soares

doi:10.1016/j.jep.2006.12.006

Cited by 247 publications

(168 citation statements)

References 35 publications

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“…The fact that L. chagasi is more resistant than L. amazonensis is known in literature. Braga et al (2007) found a IC50 value of the methanol extract of leaves from L. camara of 14 μg/mL for L. amazonensis and of 250 μg/mL for L. chagasi (Braga et al, 2007). Even though the IC50 (L. chagasi) of the EOLC is lower than that of vegetal extracts of twenty different species of plants tested against the same strain of promastigote forms used in the present work (Braga et al, 2007).…”

Section: Resultsmentioning

confidence: 55%

“…The lower susceptibility of L. chagasi is frequently found also with respect to other drugs (Morais-Teixeira et al, 2008;Braga et al, 2007) including the standard drug Amphotericin B (Table 2). The mechanism of action by which EOLC inhibits the parasite growth is still unknown, however some authors (Wink, 2008;Cabral et al, 2009;Laouer et al, 2009) have shown the biological activity of the major compounds present in this EOLC against other eukaryotes parasites and prokaryote microorganisms, which are distant from Leishmania genus, as punctuated before, this work is the first one that demonstrates the activity of EOLC on promastigote culture of Leishmania, so it is relevant to consider some previous studies which enables the construction of few hypothesis to contribute for explanation of EOLC activity on this protozoa culture.…”

Section: Resultsmentioning

confidence: 94%

“…The literature has shown that the essential oil from leaves of L. camara (EOLC) was able to play as an anti-inflammatory agent in vivo and has a potential antimicrobial action in vitro (Deena & Thoppil, 2000). Braga et al (2007) showed that the methanol extract from leaves of L. camara had a potential activity in vitro against promastigote forms of Leishmania amazonensis.…”

Section: Introductionmentioning

confidence: 99%

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Essential oil from leaves of Lantana camara: a potential source of medicine against leishmaniasis

Machado

Valente²,

Lesche³

et al. 2012

Rev. bras. farmacogn.

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Abstract:Leishmaniasis is an infection of viscera or tegument caused by protozoa Leishmania sp. The extensive period required for the treatment, which involves the use of toxic medicines, leads patients to drop treatment increasing the development of resistant forms of Leishmania sp. Lantana camara L., Verbenaceae, is a tropical plant native from America. Folk uses have been described for treatment of tumors, tetanus, rheumatism and malaria. This study evaluates the leishmanicidal activity of the essential oil of leaves from L. camara on promastigote forms of Leishmania chagasi and L. amazonensis and its toxic effects on Artemia salina (brine shrimp test), macrophage cultures and BALB/c mice. The chemical composition was evaluated using the gas chromatography coupled with mass spectrometer (GC-MS). Thirty substances, mostly mono and sesquiterpenes were identifi ed. The most representative constituents were: germacrene D (24.90%), farnesene derivatives (22%) and (E)-cariophylene (14.31%). Bioassays revealed a signifi cant leishmanicidal activity of essential oil against L. amazonensis (IC50 0.25 μg/ mL) and a potential toxic effect on Brine shrimp (LC50 10 μg/mL) and macrophage assays (CC50 4 μg/mL), while there was no toxic manifestation on mice. The data show the relevant potential of L. camara as a source of medicine for leishmaniasis treatment.

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Section: Resultsmentioning

confidence: 55%

Section: Resultsmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Essential oil from leaves of Lantana camara: a potential source of medicine against leishmaniasis

Machado

Valente²,

Lesche³

et al. 2012

Rev. bras. farmacogn.

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“…In the last years, several works showed the leishmanicidal effect of some Brazilian plant extracts or essential oils (Santin et al 2009, Albernaz et al 2010, Alviano et al 2012, Brito et al 2013. In a previous work, we reported the antileishmanial activity of extracts of 20 plants from the Brazilian flora (Braga et al 2007). Due to our continuous search for new alternatives for the treatment of leishmaniasis, this study aimed to investigated the leishmanicidal activity of 17 plant methanolic extracts against four Leishmania species and murine macrophages.…”

Section: Introductionmentioning

confidence: 83%

Antileishmanial activity of some Brazilian plants, with particular reference to Casearia sylvestris

Antinarelli

Pinto

Scio

et al. 2015

An. Acad. Bras. Ciênc.

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Leishmaniasis is a complex of diseases caused by Leishmania protozoa which treatment is restricted to a limited number of drugs that exhibit high toxicity, collateral effects and are often costly. There are a variety of tropical plants distributed in Brazil, and for many poor people the therapy for several diseases is based mainly on the use of traditional herbal remedies. In this work, the cytotoxic activity of 17 plant methanol extracts was evaluated on several Leishmania species and murine macrophages. Among them, the extract of Casearia sylvestris, Piptocarpha macropoda, Trembleya parviflora, Samanea tubulosa and Plectranthus neochilus showed a promissing leishmanicidal activity, exhibiting IC 50 values below of 20 µg/mL against at least one species of Leishmania. Casearia sylvestris showed the most expressive activity against all promastigote forms of Leishmania species (IC 50 values of 5.4 µg/mL, 5.0 µg/mL, 8.5 µg/mL and 7.7 µg/ mL for L. amazonensis, L. braziliensis, L. chagasi and L. major, respectively), being more effective than the reference drug miltefosine. In spite of the cytotoxic effect on macrophages (CC 50 value of 5.2 µg/mL), C. sylvestris exhibited a strong inhibition against intracellular amastigotes of L. braziliensis (IC 50 value of 1.3 µg/ mL). Further studies, including bio-guided fractionation will be conducted to identify the active compounds.

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“…V. polyanthes leaves have been studied and their pharmacological properties such as antinociceptive and anti-inflammatory (Temponi et al, 2012), antihypertensive and diuretic (Silveira et al, 2003a;Silveira et al, 2003b), antiulcerogenic (Barbastefano et al, 2007), antifungal and leishmanicidal (Braga et al, 2007), mutagenic and antimycobacterial (Oliveira et al, 2007;Jorgetto et al, 2011), have been established. Fixed acids, chalcones, aurones, alkaloids, aminoacids, coumarins, steroids, quinones, triterpenes, Sesquiterpene lactones, anthraquinones, flavonoids, chlorogenic acids, saponins and tannins were detected in V. polyanthes (Souza et al, 2008;Silva et al, 2010;Temponi et al, 2012;Igual et al, 2013) and may be responsible for their activity.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of crude extracts and fractions of Vernonia polyanthes Less (assa-peixe) flowers

Waltrich

Hoscheid

Prochnau

2015

Rev. bras. plantas med.

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Antimicrobial activity of crude extracts and fractions of Vernonia polyanthes Less (assa-peixe) flowers. Vernonia polyanthes, known as "assa-peixe", is a plant native to Brazil, and the decoction or infusion of its flowers, roots and leaves are used in folk medicine, being considered sources of diuretic, balsamic, anti-rheumatic substances, and are used in cases of bronchitis and persistent cough. The aim of the study was to evaluate the antimicrobial activity of the extract/fractions obtained by methanol maceration and infusion of V. polyanthes flower, also including qualitative identification of flower compounds, through phytochemical evaluation, using colorimetric tests. Identification tests for the presence of anthraquinones, tannins, flavonoids, saponins and alkaloids were performed. Microbiological evaluation was made through agar diffusion assay, using Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa as test organisms. From the performed colorimetric tests it was possible to verify the presence of tannins and flavonoids in both extracts. Alkaloids were also observed in the macerated extract. The ethyl acetate fraction from the agar diffusion assay, from both extracts, presented microbial activity over Staphylococcus aureus. It was possible to qualitatively identify the floral compounds, and to show the differences between extraction methods, being methanol considered the best solvent to the extraction.Keywords: Assa-peixe; Cambará; Medicinal plants; Phytochemical profile. RESUMO: Atividade antimicrobiana de extrato bruto e de frações de flores de Vernonia polyanthes Less (assa-peixe).Vernonia polyanthes, conhecida como assa-peixe, é planta nativa do Brasil e suas flores, raízes e folhas, em decocção ou infusão, são empregadas na medicina caseira como diuréticas, balsâmicas, e anti-reumáticas, usadas nos casos de bronquite e tosse persistente. O objetivo do trabalho foi avaliar a atividade antimicrobiana do extrato/ frações obtidos por maceração em metanol e infusão de flores de V. polyanthes, incluindo também a identificação qualitativa dos compostos presentes nas flores, através da avaliação fitoquímica, empregando testes colorimétricos. Foram realizados testes para identificação de antraquinonas, taninos, flavonóides, saponinas e alcalóides. A avaliação microbiológica foi realizada por meio de ensaio de difusão em ágar, empregando Escherichia coli, Staphylococcus aureus e Pseudomonas aeruginosa. A partir dos testes colorimétricos foi possível verificar a presença de taninos e flavonóides em ambos os extratos. Ainda, no extrato macerado, foi observada a presença de alcalóides. No ensaio de difusão em ágar, a fração acetato de etila de ambos os extratos, apresentou atividade antimicrobiana sobre Staphylococcus aureus. Foi possível identificar qualitativamente os compostos da flor, demonstrando diferença entre as técnicas de extração, sendo que o metanol resultou em melhor poder extrativo.

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Antileishmanial and antifungal activity of plants used in traditional medicine in Brazil

Cited by 247 publications

References 35 publications

Essential oil from leaves of Lantana camara: a potential source of medicine against leishmaniasis

Essential oil from leaves of Lantana camara: a potential source of medicine against leishmaniasis

Antileishmanial activity of some Brazilian plants, with particular reference to Casearia sylvestris

Antimicrobial activity of crude extracts and fractions of Vernonia polyanthes Less (assa-peixe) flowers

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