Antimicrobial activity of crude extracts and fractions of Vernonia polyanthes Less (assa-peixe) flowers

Waltrich, K.K.; Hoscheid, Jaqueline; Prochnau, Inara Staub

doi:10.1590/1983-084x/14_110

Cited by 10 publications

(13 citation statements)

References 22 publications

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“…This study involved an evaluation of the in vitro antibacterial activity against P. aeruginosa, E. coli, and S. aureus, but only the last species exhibited inhibition to the concentration used (30 mg mL −1 ). There is no consensus on the acceptable level of inhibition for natural products when compared to antibiotic standards since the material is a mixture of compounds and not a single compound in isolation (Almeida et al, 2014;Waltrich et al, 2015).…”

Section: Total Phenolic Content Antibacterial and Antioxidant Activitymentioning

confidence: 99%

Extraction of Cecropia Pachystachya Leaves by Supercritical Carbon Dioxide: Kinetics, Phytochemical Characterization, Antibacterial and Antioxidant Activities

Hoscheid

Otênio

Lourenço

et al. 2020

JAS

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Cecropia pachystachya Trécul is popularly known in Brazil as embaúva or embaúba. Pharmacological activities were reported in extant studies including the development of anxiolytic, antidepressant, hypoglycemic, antioxidant, cardiotonic, sedative, anti-inflammatory, antimicrobial and leishmanicidal properties for aqueous and alcoholic extracts prepared by infusion or maceration of C. pachystachya leaves. However, there is a paucity of studies examining extracts of leaves of C. pachystachya obtained by supercritical fluid technology. This study evaluated the extraction of Cecropia pachystachya oil using supercritical carbon dioxide (CO2). Extractions were performed at 35 °C, 45 °C, and 55 °C and 14 MPa, 17 MPa, and 20 MPa. The extraction yield, characteristics of the oil, antioxidant and antibacterial potentials were evaluated. The highest extraction yield obtained from C. pachystachya leaves was 1.48% using supercritical CO2 at 55 °C and 20 MPa. The main identified compounds include terpenoids (ursolic and pomolic acids, squalene and β-sitosterol). The most promising results for antibacterial activity relative to Staphylococcus aureus and antioxidant potentials were observed on the extract obtained at 55 °C and 14 MPa. The mathematical Sovová model satisfactorily represented the experimental data.

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Section: Total Phenolic Content Antibacterial and Antioxidant Activitymentioning

confidence: 99%

Extraction of Cecropia Pachystachya Leaves by Supercritical Carbon Dioxide: Kinetics, Phytochemical Characterization, Antibacterial and Antioxidant Activities

Hoscheid

Otênio

Lourenço

et al. 2020

JAS

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“…The antimicrobial assay for ciprofloxacin samples was carried out using the agar well-diffusion assay as described by Waltrich et al, 22 against Salmonella typhi, Pseudomonas aeruginosa (ATCC 27853), and Escherichia coli (ATCC 25922) while agar dilution method as described by Okore 23 was used to test for the antibacterial potency of the metronidazole samples against Peptostrepocuccus spp and Lactobacillus spp.…”

Section: Antibacterial Evaluation Of the Test Samplesmentioning

confidence: 99%

<p>Pharmaceutical Quality of Selected Metronidazole and Ciprofloxacin Infusions Marketed in South Eastern Nigeria</p>

Oli

Ibeabuchi

Enweani

et al. 2020

DHPS

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Background: Pharmaceutical products need to be of good quality and it is even more critical when it comes to life saving medicaments like infusions. Objective: This research surveyed the quality fitness of some ciprofloxacin and metronidazole infusion samples marketed in Southeastern of Nigeria. Methods: Using Official Compendial methods, microbiological quality, active pharmaceutical ingredients quantitation, pH and particle count tests were evaluated on eighty infusion bottles (from eight pharmaceutical companies) of each of the two drugs. Results: Out of the sixteen brands tested, 2 metronidazole brands and 1 ciprofloxacin brand (representing 18.75% of the total 16 brands/makes) were contaminated while the remaining 13 brands (81.25%) were found sterile. The active pharmaceutical ingredients quantitative assay showed that all the brands of ciprofloxacin infusion were between the 95% and 105% limit of label claim while one metronidazole brand has <95-110% limit label claim. Six brands each of the two drugs evaluated fall below the acceptable pH range [ciprofloxacin (3.5-4.6) and metronidazole (4.8-5.2)], while the other two brands of both drugs passed the test. In the antibacterial study, Pseudomonas aeruginosa and Escherichia coli were susceptible to the ciprofloxacin (5 µg). However, Salmonella typhi recorded inhibition zone diameters within resistant and intermediate range. Peptostrepococcus spp was susceptible (at minimum inhibitory concentrations of 100 µg/mL) to all the brands of metronidazole, while none of the brands were effective on Lactobacillus spp. All the brands passed the test for particulate contamination. The particles size range was <10µm. Conclusion: About eighty-one percent (81.25%) of the infusions have acceptable good microbiological quality. However, 18.75% that failed the tests is a concern knowing that these are lifesaving products.

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“…The antibiotic used in this study was carried out as [12]. Hence, the antibacterial potential of the fractions can be assume as good, indeed, although the zone diameters obtained are much lower than results of antibiotics.…”

Section: Resultsmentioning

confidence: 88%

Antibacterial Activity of Fractions of Ethyl Acetate Extract of Garcinia Lattissima Miq. Fruits

Ambarwati

Malik

Listari

et al. 2017

Asian J Pharm Clin Res

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Objectives: The emergence of new infections and increase in bacterial drug resistance has created a serious need for the expansion of new antibacterial agents from natural sources. The study was carried out to evaluate the antibacterial activity of fractions of ethyl acetate extract of Garcinia latissima Miq. fruits. Methods:The fractionation was done using a silica gel column and organic solvents as the eluent, i.e., n-hexane, ethyl acetate, and methanol. All fractions were assayed for antibacterial activity, which was done by performing disc diffusion for growth inhibition against Bacillus subtilis and Pseudomonas aeruginosa. In addition, the growth inhibition activity was also examined by performing bioautography assay using pre-coated silica gel 60 GF 254 plates as the stationary phase. Fractions A-F were eluted using n-hexane:chloroform (1:4), while Fractions G-K were used ethyl acetate:dichloromethane (4:1) as the mobile phase. The plate was visualized by ultraviolet at λ 254 nm and 366 nm, while the other one was contacted with the inoculated agar medium to observe zone inhibition. Further, the minimum inhibitory concentration (MIC) value was determined by performing microdilution. Results:The result showed that the antibacterial activity of all fractions was more active at inhibiting the growth of B. subtilis than P. aeruginosa, mainly for Fractions H and J. However, the strongest antibacterial activity was showed by Fractions H and J against B. subtilis, MIC 312.5 µg/mL (lower than reference, which is erythromycin antibiotic (25 µg/mL), followed by Fraction D against B. subtilis MIC 625 µg/mL, Fraction K against P. aeruginosa MIC 625 µg/mL, whereas Fractions C, E, and G against B. subtilis, and Fraction E against P. aeruginosa also showed low MIC values (1.250 µg/mL). Conclusions:The results indicated that fractions of G. latissima Miq. fruit ethyl acetate extracts possessed antibacterial activity. The most active fraction that inhibited the growth of B. subtilis was shown by Fractions H and J; these fractions have the potential to be developed as new antibacterial agents.

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Antimicrobial activity of crude extracts and fractions of Vernonia polyanthes Less (assa-peixe) flowers

Cited by 10 publications

References 22 publications

Extraction of Cecropia Pachystachya Leaves by Supercritical Carbon Dioxide: Kinetics, Phytochemical Characterization, Antibacterial and Antioxidant Activities

Extraction of Cecropia Pachystachya Leaves by Supercritical Carbon Dioxide: Kinetics, Phytochemical Characterization, Antibacterial and Antioxidant Activities

<p>Pharmaceutical Quality of Selected Metronidazole and Ciprofloxacin Infusions Marketed in South Eastern Nigeria</p>

Antibacterial Activity of Fractions of Ethyl Acetate Extract of Garcinia Lattissima Miq. Fruits

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