Antiinflammatory effect of mace, aril of Myristica fragrans Houtt., and its active principles.

Ozaki, Yukihiro; Soedigdo, Soekeni; Wattimena, Yoke Rosina; Suganda, Asep Gana

doi:10.1254/jjp.49.155

Cited by 63 publications

(45 citation statements)

References 27 publications

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“…(Myristicaceae) (MF) is an evergreen aromatic tree commonly known as nutmeg or mace, and has been used traditionally as a spice and for medicinal purposes as carminative, digestive, and expectorant in the Indian system of medicine. In the recent literature, MF has been investigated for its hypolipidemic, antithrombotic, antiplatelet aggregating, antifungal, aphrodisiac, anxiogenic, anti-ulcerogenic, antitumor, and anti-inflammatory activities (Ozaki et al, 1989;Park et al, 1998;Capasso et al, 2000;Sonavane et al, 2001Sonavane et al, , 2002Morita et al, 2003;Chung et al, 2006). MF has been reported to contain 25-30% fixed oils and 5-15% volatile oils such as camphene, elemicin, eugenol, isoelemicin, isoeugenol, methoxyeugenol, pinen, sabinene, safrol, and also chemical substances such as dihydroguaiaretic acid, myristic acid, myristicin, and ligan (Forrest & Heacock, 1972;Isogai et al, 1973;Janssen et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Antidiabetic and Antihyperlipidemic Effects ofMyristica fragrans. in Animal Models

Arulmozhi

Kurian

Addepalli

et al. 2007

Pharmaceutical Biology

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The effect of the hydroalcoholic extract of fruits of Myristica fragrans Houtt. (Myristicaceae) was investigated on chlorpromazine-induced glucose and triglyceride elevations in male Swiss albino mice. After 7 days of oral administration, the extract, at doses of 150 and 450 mg=kg, ameliorated the metabolic abnormalities caused by chlorpromazine as evidenced by significant reduction of glucose and triglyceride (TG) levels (maximal effect of 41% and 53% reduction of glucose and TG, respectively, at 450 mg dose, p < 0.01). The standard antidiabetic rosiglitazone at 10 mg significantly (p < 0.01) reduced the TG (63%) and glucose (40%) levels in this model, while the standard antidiabetic glimepiride has exhibited 55% and 16% reduction in TG and glucose, respectively. In rats fed a high-cholesterol diet, Myristica fragrans extract significantly reduced the elevated TG (47% reduction at 450 mg, p < 0.01) and cholesterol (66.7% reduction at 450 mg, p < 0.01), and also exhibited a reduction in hepatic TG secretion after tyloxapol administration. These data suggest that Myristica fragrans extract ameliorates hyperglycemia and abnormal lipid metabolism in animal models.

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Section: Introductionmentioning

confidence: 99%

Antidiabetic and Antihyperlipidemic Effects ofMyristica fragrans. in Animal Models

Arulmozhi

Kurian

Addepalli

et al. 2007

Pharmaceutical Biology

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“…According to Ozaki et al [5] with some modification. 50 gram of the crude powder of seeds was refluxed with 350 ml of 70% methanol (1:7) in soxhlet apparatus for 8 hours.…”

Section: Preparation Of the Nutmeg Methanolic Extractmentioning

confidence: 99%

“…The residue was dissolved in 1-2 ml hexane and the mobile phase was n-hexane: benzene: ethyl acetate: menthol, 1:1:1:1. as shown in (Scheme (1)). [5].…”

Section: Columnmentioning

confidence: 99%

“…The concentration of ether layer by rotary evaporator is a strong indicator that many related compounds have been removed. The repeated washing increases the purity of myristicin, because in first time both solutions reached were saturated with substances [5].…”

Section: Separation and Purification Of Myristicin Separationmentioning

confidence: 99%

“…It is further used to treat malarial debility. In Indonesia, mace is also used as an analgesic and as medicine for rheumatism [5].…”

Section: Introductionmentioning

confidence: 99%

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Extraction and Purification of Terpenes from Nutmeg (myristica fragrans)

Al-Jumaily

Al-Amiry

2012

JNUS

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This study was conducted with the aim to extract and purify the essential oil containing terpenes from the dried seeds of nutmeg myristica fragrans available in Iraqi markets.The essential oil was extracted by steam distillation method with a yield of 4.7 -7.5 gm / 100 gm, and the resultant oil was subjected to sensory and physical evaluation. It has a special spicy odor, slightly pungent taste, colorless to pale yellow in color, not dissolved in water but dissolved in organic solvents like ethanol, hexane, chloroform and ether. The specific gravity, refractive index and optical rotation were (0.890 gm / ml), (1.4822) and (+22˚), respectively. Gas chromatography is used for the quality and quantity evaluation of the essential oil constituents which revealed the presence of 49 volatile compounds. The methanolic extract obtained by reflux the seeds powder with 70% methanol in soxhlet apparatus and yield 12.8%. The extract was subjected to phytochemical detection which confirms the presense of alkaloids, terpenes, flavonoids, glycosides, tannins and resins, with the exception of saponins and coumarines which gave negative tests. Myristicin was purified from the methanolic extract of nutmeg dried seeds, which were detected in the essential oil by TLC and vanillin-H2SO4 reagent. The purified myristicin was obtained after the application of adsorption chromatography on silica gel column and detected on T.L.C. plate with standard myristicin.Both the essential oil and purified myristicin were subjected to gas chromatography which showed the presence of myristicin in the oil in a concentration of 6%.

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Macelignan protects HepG2 cells against tert‐butylhydroperoxide‐induced oxidative damage

et al. 2007

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In this study, we investigated the protective effect of macelignan, isolated from Myristica fragrans Houtt. (nutmeg) against tert-butylhydroperoxide (t-BHP)-induced cytotoxicity in a human hepatoma cell line, HepG2. The tetrazolium dye colorimetric test (MTT test) and lactate dehydrogenase (LDH) assay were used to monitor cell viability and necrosis, respectively. Lipid peroxidation [malondialdehyde (MDA) formation] was estimated by the fluorometric method. Intracellular reactive oxygen species (ROS) formation was measured using a fluorescent probe 2',7'-dichlorofluorescein diacetate (DCFH-DA), and DNA damage was detected using single cell gel electrophoresis (comet assay). The results showed that macelignan significantly reduced the cell growth inhibition and necrosis caused by t-BHP. Furthermore, macelignan ameliorated lipid peroxidation as demonstrated by a reduction in MDA formation in a dose-dependent manner. It was also found that macelignan reduced intracellular ROS formation and DNA damaging effect caused by t-BHP. These results strongly suggest that macelignan has significant protective ability against oxidative damage caused by reactive intermediates.

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Antiinflammatory effect of mace, aril of Myristica fragrans Houtt., and its active principles.

Cited by 63 publications

References 27 publications

Antidiabetic and Antihyperlipidemic Effects ofMyristica fragrans. in Animal Models

Antidiabetic and Antihyperlipidemic Effects ofMyristica fragrans. in Animal Models

Extraction and Purification of Terpenes from Nutmeg (myristica fragrans)

Macelignan protects HepG2 cells against tert‐butylhydroperoxide‐induced oxidative damage

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