Antiinflammatory and antinociceptive activities of zingiber officinale roscoe essential oil in experimental animal models

Abstract

Ginger, Zingiber officinale Roscoe (Zingiberaceae), in folk medicine has been used against pain, inflammation, arthritis, urinary infections, and gastrointestinal disorders. [1] The oil of ginger is a mixture of constituents, consisting of monoterpenes (phellandrene, camphene, cineole, citral, and borneol) and sesquiterpenes (zingiberene, zingiberol, zingiberenol, ß-bisabolene, sesquiphellandrene, and others). Aldehydes and alcohols are also present. [1,2] Gingerol and its analogs found in rhizome extracts are responsible for many pharmacological activities. [1] Few works have reported the properties of ginger essential oil (GEO). However, several types of terpene compounds are known to present antiinflammatory and antinoceptive activities. [1,2] The aim of the present study was to evaluate the antiinflammatory and analgesic effects of GEO administered orally in rodents. Groups of 10 male Swiss mice (25-30 g) and male Wistar rats (190-230 g) were used for evaluation of the antinoceptive and antiinflammatory effects, respectively. All animals were housed in groups of five and maintained in standardized conditions (12/12 h light/dark cycle, 25°C) with free access to water and food. The protocol for these experiments was approved and was in accordance with the guidelines of the Brazilian Committee of Animal Experimentation. Fresh rhizomes of Z. officinale were collected from the herbarium of the State University of Maringá, identified, and authenticated. GEO was obtained from 250 g of rhizomes by conventional steam distillation using Clevenger apparatus during 3 h. The oil obtained was kept refrigerated and protected form direct light. Pleurisy was induced in anesthetized mice by intraperitoneal (i.p.) injection of carrageenan (200 µg/cavity). Four hours later, the rats were sacrificed and the exudate was collected to determine the total volume and leukocyte number. Exudates smears were prepared, air-dried, and fixed with Rosenfeld stain for leukocyte differential count. The parameters studied were leukocyte migration and fluid leakage. GEO (100, 200, and 500 mg/kg, p.o.) and indomethacin (5 mg/kg, p.o.) were administered 30 min before the test. The antinociceptive activity of the GEO was assessed using the writhing test. Acetic acid solution (10 ml/kg, 0.6%) was i.p. injected and abdominal muscles constriction together with stretching of the hind limbs was counted over a period of 20 min, starting immediately after acetic acid injection. GEO (50, 100, and 200 mg/kg, p.o.) and indomethacin (5 mg/kg, p.o.) were administered 30 min before the acid injection. Antinociceptive activity was expressed as the percentage of

RENDIMENTO DO ÓLEO ESSENCIAL DE ZINGIBER OFFICINALE EM RESPOSTA A DIFERENTES PROCESSAMENTOS E TEMPOS DE EXTRAÇÃO Yield of Zingiber officinale essential oil in response to different processing methods and extraction times

“…Entre os monoterpenos destacaram-se o geranial e o zingereno (MENON et al, 2007). Mono e sesquiterpenos podem estar relacionados aos efeitos anti-inflamatório e analgésico do óleo essencial de gengibre (VENDRUSCOLO et al, 2006).…”

Ginger From Ancient Times to the New Outlook

“…Prior studies showed that a solution consisted of 5% ginger essential oil in grape seed oil, applying nasocutaneously could prevent postoperative nausea (11). Anti-inflammatory, immonomodulatory, analgesic and antibacterial activity of volatile oil of ginger has been studied as well (12-14). Pungent taste of fresh ginger is mainly due to gingerols, especially (6)- gingerol, a phenolic alkanone and pungent compound found in fresh ginger (10).…”

Otimização de processos biotecnológicos para a produção de compostos de aroma a partir de substratos monoterpênicos

“…Quanto as atividades biológicas, esse composto apresenta grande ação anti-inflamatória, anticolesterogência e anti-tumoral, além de recentes pesquisas que comprovaram propriedades sedativas, efeitos sobre a ansiedade e agressividade em modelos animais submetidos a inalação (aromaterapia) de linalol(HUO et al, 2013;WU et al, 2014c; KLADNIEW et al, 2014;MIYASHITA & SADZUKA, 2013;LINCK et al, 2010).Entre os moterpenos monocíclicos, o timol (2-isopropil-5-metilfenol) (13), composto presente em Thymus vulgaris e Monarda punctate , tem sido amplamente utilizando na medicina devido as suas propriedades antimicrobianas, ação antisséptica e muitos estudos descrevem também ação antioxidante, antiespasmódica, anti-agregante plaquetário e efeitos anti-inflamatórios(AESCHBACH et al, 1994;SHAPIRO & GUGGENHEIM, 1995; WEI et al, 2014;GAVLIAKOVA et al, 2013;OKAZAKI;KAWAZOE;TAKAISHI, 2002; FACHINI-QUEIROS et al, 2012;ZHOU et al, 2014). anti-inflamatoria, estimulante da resposta imune e recentemente foi comprovada a atividade anti-hiperalgesica e antidepressiva do α-felandreno em estudos in vivo(SINGH;SINGH;MAURYA, 2002;NAPOLI;CURCURUTO;RUBERTO, 2010;GILLES et al, 2010;VENDRUSCOLO et al, 2006;LIMA et al, 2012;LIN et al, 2013;PICCINELLI et al, tratamento de diversos tipos de câncer(McGARVEY & CROTEAU, 1995;IMAMURA et al, 2014). Apresenta grande potencial na atividade regressora de diferentes tumores, incluindo mamários, pancreáticos e cerebrais (YURI…”