Antihypertensive Effects of Nicotianamine from Soybean Broth in Spontaneously Hypertensive Rats

Abstract

The effect of the ACE inhibitor nicotianamine (NA), from soybean broth (SB), on blood pressure was investigated in spontaneously hypertensive rats (SHR) upon single and long-term administration. The IC 50 value of NA from SB was 0.69 μmol/L. Single oral dose of NA (0.9 mg, 4.5 mg and 9.0 mg/kg body weight) decreased blood pressure 1 h after administration, and blood pressure returned to the control level 3 h after administration. Long-term oral dose of NA (0.9 mg and 4.5 mg/kg body weight) decreased blood pressure for 4 weeks after administration, while that of NA (9.0 mg/kg body weight) was decreased for the full 8-weeks feeding period. At 8 weeks after administration, serum NA content in SHR was determined by amino acid analyzer and revealed that NA was not detected in the blood of SHR (0.9 mg and 4.5 mg/kg body weight group), while 32.6 ± 7.3 μg/dL NA was detected in the 9.0 mg/kg body weight group. It was suggested that NA absorbed from the intestine decreased the systolic blood pressure (SBP) in SHR, and an appropriate NA level (9.0 mg/kg body weight group) may provide long-term antihypertensive effects upon administration.

Association of Estimated Salt and Miso Intake with the Prevalence of Obesity in People with Type 2 Diabetes: A Cross-Sectional Study

“…The proportion of obesity in participants with high estimated salt intake was higher than that for those without high estimated salt intake (44.7% ( n = 67/150) vs. 27.3% ( n = 41/150), p = 0.003). Furthermore, high salt intake estimated from urine was related to obesity after adjusting for sex, age, insulin treatment, exercise, smoking, alcohol, diabetes duration, HbA1c, and energy intake (adjusted OR: 1.31 (95% CI: 1.10–1.56), p = 0.003), which is the same as the results of previous studies [ 5 , 7 , 8 , 42 , 43 , 44 ]. However, among those with a high urine-estimated salt intake, habitual miso consumption was not associated with obesity.…”

“…Miso increases sodium excretion in the kidney and inhibits sodium reabsorption in the renal tubules. Nicotinamide extracted from soybeans inhibits ACE activity, and miso has the same effect [ 44 , 45 ]. Therefore, miso enhances sodium excretion, and people who consume it habitually might have been evaluated as having an excessive dietary salt intake.…”

Angiotensin-I Converting Enzyme Inhibitor Activity in Japanese Burdock Roots

“…These kinetic parameters suggest that NA competitively bound to the active site by chelating Zn in ACE. The IC 50 of NA was found to be 481 nM, which is similar to the values reported (Kinoshita, Yamakoshi, & Kikuchi, 1993;Takenaka, Murayama, Furusho, & Takenaka, 2009). Although the inhibition activity of NA was considerably less than captopril (IC 50 = 22 nM) (Fujita & Yoshikawa, 1999), the ingestion of NA from soybean broth was able to ameliorate high blood pressure in spontaneously hypertensive rats (Takenaka et al, 2009).…”

Green Asparagus (Asparagus officinalis) Prevented Hypertension by an Inhibitory Effect on Angiotensin-Converting Enzyme Activity in the Kidney of Spontaneously Hypertensive Rats

“…All of the tested diets produced antihypertensive effects. Nicotianamine was not detected in the blood of SHR given the 20 mg and 100 mg/kg diets; however, it was detected in blood from SHR given the 200 mg/kg diet . In our study, a test diet containing 40 mg of 2″-hydroxynicotianamine/kg was given to SHR for 10 weeks, administered as 4.3 mg of 2″-hydroxynicotianamine/kg body weight/day.…”