Antihypertensive Effect of Piper sarmentosum in L-NAME-Induced Hypertensive Rats

Alwi, Nik Aloesnisa Nik Mohd; Zakaria, Zaiton; Karim, Aminuddin Abdul Hamid; Nordin, Nor Azlin Mohd; Ugusman, Azizah

doi:10.17576/jsm-2018-4710-18

Cited by 8 publications

(6 citation statements)

References 32 publications

(36 reference statements)

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“…The dose of dexamethasone (20 μg/kg/day) was chosen as it had been shown to induce hypertension in rats [16]. The dose and treatment duration for AEPS (500 mg/kg/day for 28 days) were adopted from previous studies which showed that AEPS at similar dose and treatment duration successfully reduced BP and increased NO in spontaneously hypertensive and L-NAME-induced hypertensive rats [12,23].…”

Section: Animals and Experimental Designmentioning

confidence: 99%

“…Systolic and diastolic blood pressure in conscious, restrained rats were measured at baseline (day 0), day 14 and day 28 using CODA TM non-invasive tail cuff system (Kent Scientific Corporation, USA). The interval of blood pressure measurements every fortnight was adopted from previous studies [12,23]. The rats were placed on a heated chambers at 37 ºC during the measurement.…”

Section: Measurement Of Blood Pressurementioning

confidence: 99%

“…At least five readings were recorded for each rat. The highest and the lowest readings were manually discarded and the average of the remaining three readings were taken as the blood pressure value [23].…”

Section: Measurement Of Blood Pressurementioning

confidence: 99%

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Piper sarmentosum attenuates dexamethasone-induced hypertension by stimulating endothelial nitric oxide synthase

Ugusman¹,

Fadze²,

Hamid³

et al. 2020

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Reduced endothelial nitric oxide (NO) synthesized by endothelial nitric oxide synthase (eNOS) in the vasculature is a feature of endothelial dysfunction. Dexamethasone causes secondary hypertension by inhibiting eNOS activity. Piper sarmentosum is an herb with anti-hypertensive effect. The aim of this study was to evaluate the antihypertensive effect of aqueous extract of P. sarmentosum (AEPS) in dexamethasone (Dex)-induced hypertensive rats. A total of 30 male Sprague Dawley rats were divided into five groups including control, AEPS (500 mg/kg/day, orally), Dex (20 μg/kg/day, subcutaneously), Dex (20 μg/kg/day) + AEPS (500 mg/kg/day) and Dex (20 μg/kg/day) + captopril (40 mg/kg/day, orally). Blood pressure was measured using tail-cuff method at baseline and fortnightly thereafter. The rats were sacrificed and the serum was collected to quantify the amount of NO after 28 days of treatment. Aortic samples were homogenized for measurement of eNOS mRNA expression, protein level and activity. Treatment of Dex-induced hypertensive rats with AEPS lowered systolic blood pressure (P < 0.001) and diastolic blood pressure (P < 0.01), increased eNOS mRNA expression (P < 0.01), eNOS protein (P < 0.01), eNOS activity (P < 0.05) and NO level (P < 0.05). In conclusion, AEPS reduces the blood pressure of Dex-induced hypertensive rats as it shows positive effects on eNOS and NO production.

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Section: Animals and Experimental Designmentioning

confidence: 99%

Section: Measurement Of Blood Pressurementioning

confidence: 99%

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Piper sarmentosum attenuates dexamethasone-induced hypertension by stimulating endothelial nitric oxide synthase

Ugusman¹,

Fadze²,

Hamid³

et al. 2020

jrp

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“…NO is a potent vasodilator; thus, the improved vasorelaxation observed in rats treated with AEPS is most likely contributed by the enhanced NO levels. AEPS also increases NO levels in spontaneously hypertensive rats and L-N G -nitro arginine methyl ester-induced hypertensive rats (Zainudin et al, 2015;Alwi et al, 2018). AEPS stimulates the synthesis of NO by increasing mRNA expression, protein level, and eNOS activity in ECs (Ugusman et al, 2010).…”

Section: Discussionmentioning

confidence: 93%

Piper sarmentosum Roxb. Attenuates Vascular Endothelial Dysfunction in Nicotine-Induced Rats

et al. 2021

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Exposure to cigarette smoke is an important risk factor for cardiovascular diseases. Nicotine is an addictive compound in cigarette smoke that triggers oxidative stress, which leads to vascular dysfunction. Piper sarmentosum Roxb. is a herb with antioxidant and vascular protective effects. This study evaluated the potential protective effect of the aqueous extract of P. sarmentosum leaf (AEPS) on vascular dysfunction in rats induced with prolonged nicotine administration. A total of 22 male Sprague-Dawley rats were divided into control (normal saline, oral gavage [p.o.]), nicotine (0.8 mg/kg/day nicotine, intraperitoneally [i.p.]), and nicotine + AEPS groups (250 mg/kg/day AEPS, p.o. + 0.8 mg/kg/day nicotine, i.p.). Treatment was given for 21 days. Thoracic aortae were harvested from the rats for the measurement of vasorelaxation, vascular nitric oxide (NO) level, and antioxidant level and the assessment of vascular remodeling. Rats treated with AEPS had improved vasorelaxation to endothelium-dependent vasodilator, acetylcholine (ACh), compared with the nicotine-induced rats (p < 0.05). The presence of endothelium increased the maximum relaxation of aortic rings in response to ACh. Compared with the nicotine group, AEPS enhanced vascular NO level (p < 0.001) and increased antioxidant levels as measured by superoxide dismutase activity (p < 0.05), catalase activity (p < 0.01), and reduced glutathione level (p < 0.05). No remarkable changes in aortic histomorphometry were detected. In conclusion, P. sarmentosum attenuates vascular endothelial dysfunction in nicotine-induced rats by improving vasorelaxation and enhancing vascular NO and antioxidant levels.

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“…There is a large body of evidence to support that the chronic inhibition of NO synthase produces high blood pressure and is related to phenotypic change and proliferation of vascular smooth muscle cells (VSMC) [5,6]. Furthermore, many in vivo studies have demonstrated that chronic inhibition of NO production by Nω-nitro-L-arginine methyl ester (L-NAME) in rats causes a significant increase in blood pressure and also enhances vascular wall thickening [7][8][9]. Consequently, diagnosis and treatment that improve the function of vascular endothelial cells would reduce the risk and rate of death from cardiovascular diseases.…”

Section: Introductionmentioning

confidence: 99%

Ethyl Rosmarinate Prevents the Impairment of Vascular Function and Morphological Changes in L-NAME-Induced Hypertensive Rats

et al. 2019

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Background and Objectives: The potent, endothelium-independent, vasorelaxant effect of ethyl rosmarinate, an ester derivative of rosmarinic acid, makes it of interest as an alternative therapeutic agent for use in hypertension. This study was designed to investigate the effect of ethyl rosmarinate on N ω -nitro-L-arginine methyl ester (L-NAME)-induced hypertensive rats. Materials and Methods: L-NAME was given orally to male Wistar rats for 6 weeks to induce hypertension concurrently with treatment of ethyl rosmarinate at 5, 15, or 30 mg/kgor enalapril at 10 mg/kg Systolic blood pressure (SBP), heart rate, and body weight of all experimental groups were recorded weekly, while the vascular sensitivity and histological changes of the aorta were evaluated at the end of the experiment. Results: For all treatment groups, the data indicated that ethyl rosmarinate significantly attenuated the SBP in hypertensive rats induced by L-NAME, with no significant differences in heart rate and body weight. In addition, the response of vascular sensitivity to acetylcholine (ACh) was improved but there was no significant difference in the response to sodium nitroprusside (SNP). Furthermore, the sensitivity of the aorta to phenylephrine (PE) was significantly decreased. The thickness of the aortic wall did not differ between groups but the expression of endothelial nitric oxide synthase (eNOS) was increased in ethyl rosmarinate-and enalapril-treated groups compared with the hypertensive group. Conclusions: Ethyl rosmarinate is an interesting candidate as an alternative treatment for hypertension due to its ability to improve vascular function and to increase the expression of eNOS similar to enalapril which is a drug commonly used in hypertension.

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Antihypertensive Effect of Piper sarmentosum in L-NAME-Induced Hypertensive Rats

Cited by 8 publications

References 32 publications

Piper sarmentosum attenuates dexamethasone-induced hypertension by stimulating endothelial nitric oxide synthase

Piper sarmentosum attenuates dexamethasone-induced hypertension by stimulating endothelial nitric oxide synthase

Piper sarmentosum Roxb. Attenuates Vascular Endothelial Dysfunction in Nicotine-Induced Rats

Ethyl Rosmarinate Prevents the Impairment of Vascular Function and Morphological Changes in L-NAME-Induced Hypertensive Rats

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