Antiherpes Effect of Dextran Sulphate Combined with Acyclovir <i>in vitro</i> and <i>in vivo</i>

Pancheva, S

doi:10.1177/095632029300400309

Cited by 6 publications

(5 citation statements)

References 11 publications

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“…Moreover, the inhibitory effect of this complex was much stronger against HSV-2 than against HSV-1. Pancheva (1993) reported that the polysaccharide dextran sulfate inhibited the HSV attachment to the target cells. The efficacy of the combination of the polysaccharide alginate and rhamnolipid PS-17 against HSV replication could be explained with their dual action on HSV replication.…”

Section: Discussionmentioning

confidence: 99%

Anti-Herpesvirus Activities of Pseudomonas sp. S-17 Rhamnolipid and its Complex with Alginate

Remichkova

Galabova

Roeva

et al. 2008

Zeitschrift Für Naturforschung C

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The rhamnolipid biosurfactant PS-17 and its complex with the polysaccharide alginate, both produced by the Pseudomonas sp. S-17 strain, were studied for their antiviral activity against herpes simplex virus (HSV) types 1 and 2. They significantly inhibited the herpesvirus cytopathic effect (CPE) in the Madin-Darby bovine kidney (MDBK) cell line. The investigations were carried out according to the CPE inhibition assay protocol. The suppressive effect of the compounds on HSV replication was dose-dependent and occurred at concentrations lower than the critical micelle concentration of the surfactant. The 50% inhibitory concentration (IC 50 ) of rhamnolipid PS-17 was 14.5 μg/ml against HSV-1 and 13 μg/ml against HSV-2. The IC 50 values of the complex were 435 μg/ml for HSV-1 and 482 μg/ml for HSV-2. The inhibitory effects of the substances were confirmed by measuring the infectious virus yields with the multicycle virus growth experimental design as well: Δlog CCID 50 of 1.84Ð2.0 against the two types of herpes simplex viruses by rhamnolipid PS-17 (20 μg/ml), and a strong reduction of the HSV-2 virus yield under the effect of the alginate complex at a concentration of 450 μg/ml. The results indicate that rhamnolipid PS-17 and its alginate complex may be considered as promising substances for the development of anti-herpetic compounds.

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Section: Discussionmentioning

confidence: 99%

Anti-Herpesvirus Activities of Pseudomonas sp. S-17 Rhamnolipid and its Complex with Alginate

Remichkova

Galabova

Roeva

et al. 2008

Zeitschrift Für Naturforschung C

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“…Pentosan polysulfate and dextran sulfate also protected mice against the scrapie agent [6], whereas i-carrageenan attained 100 % survival in mice infected with murine cytomegalovirus [7]. Dextran sulfate was also active against HSV-2 in mice [8], but, on the other hand, it was not effective in cats infected with feline leukemia virus [9], and in ducklings infected with duck hepatitis B virus [10].…”

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confidence: 98%

Antiviral Activity of a Carrageenan fromGigartina skottsbergiiagainst Intraperitoneal MurineHerpes simplexVirus Infection

et al. 2006

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The partially cyclized mu/nu-carrageenan 1C3, isolated from the red seaweed Gigartina skottsbergii, was previously shown to be a potent inhibitor of the in vitro replication of Herpes simplex virus types 1 (HSV-1) and 2 (HSV-2). Here the protective effect of 1C3 in a murine model of intraperitoneal ( i. p.) HSV-1 infection was evaluated. OF1 mice were i. p. infected with 5 x 10 (5) PFU of HSV-1 KOS strain, and the effects of different treatments with 1C3 were studied. When 30 mg/kg of body weight of 1C3 was administered by the i. p. route immediately after HSV-1 infection, 87.5 % survival of the animals was achieved (p < 0.005), associated with a delay in the mean day of death in 1C3-treated non-surviving mice. Animal survival was not improved when multiple doses of 1C3 were also given in the period 1 - 48 h post-infection, and no protection was afforded when treatment was started after 24 h of infection. When virus and compound were injected by different routes, i. p. and intravenous ( i. v.), respectively, a still significant protection was achieved (40 % survival, p < 0.05). No toxicity of 1C3 for the animals was recorded. The pharmacokinetic properties were analyzed after injection of 1C3 into the tail vein by monitoring of [ (3)H]-1C3 in plasma and organs and by a bioassay of the anti-HSV-1 activity remaining in serum after non-radioactive 1C3 inoculation. A very rapid disappearance of the compound from the blood was observed since only 5.9 - 0.9 % of the radioactivity of the initially administered [ (3)H]-1C3 appeared in the plasma between 5-300 minutes after administration. A transient peak of radioactivity was detected in the kidney 15 minutes after inoculation. The bioassay confirms the presence of the compound circulating in a biologically active form up to 1 hour after injection.

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“…Acyclovir is a purine nucleoside analog of guanosine that is well-known as a highly potent inhibitor of HSV DNA polymerase [18]. Dextran sulfate is an HSV viral entry inhibitor, which can block the interaction between viral envelope proteins and host cellular receptors [19]. These two drugs with different antiviral action modes were used as the "reference drugs".…”

Section: Wogonin Inhibits Hsv-1/2 Replication In Vitromentioning

confidence: 99%

Wogonin inhibits in vitro herpes simplex virus type 1 and 2 infection by modulating cellular NF-κB and MAPK pathways

Chu

Zhang

et al. 2020

Preprint

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Background: Wogonin, a natural flavonoid-like chemical compound, exhibits anti-inflammatory, antitumor, antiviral, neuroprotective, and anxiolytic effects by modulating a variety of cellular signaling pathways including PI3K-Akt, p53, nuclear factor κB (NF-κB), mitogen-activated protein kinase (MAPK) pathways. In this study, its antiviral effect against herpes simplex virus (HSV) type 1 and 2 (HSV-1 and HSV-2) replication was investigated.Results: Wogonin suppressed HSV-2-induced cytopathic effect (CPE) and reduced viral mRNA transcription, viral protein synthesis, and infectious virion particle titers in a dose-dependent manner. A time-of-drug-addition assay demonstrated that wogonin acted as a postentry viral inhibitor. Wogonin also significantly reduced HSV-induced NF-κB and MAPK pathway activation, which has previously been demonstrated to be important for viral replication. Conclusions: Our results suggest that the anti-herpes effect of wogonin may be mediated by modulation of cellular NF-κB and JNK/p38 MAPK pathways and imply that wogonin may be useful as an anti-HSV agent.

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Antiherpes Effect of Dextran Sulphate Combined with Acyclovir in vitro and in vivo

Cited by 6 publications

References 11 publications

Anti-Herpesvirus Activities of Pseudomonas sp. S-17 Rhamnolipid and its Complex with Alginate

Anti-Herpesvirus Activities of Pseudomonas sp. S-17 Rhamnolipid and its Complex with Alginate

Antiviral Activity of a Carrageenan fromGigartina skottsbergiiagainst Intraperitoneal MurineHerpes simplexVirus Infection

Wogonin inhibits in vitro herpes simplex virus type 1 and 2 infection by modulating cellular NF-κB and MAPK pathways

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