AntiHepatitis C Virus Activity of Alectryon serratus Leaves Extract

Tumewu, Lidya; Apryani, Evhy; Santi, Mei Ria; Wahyuni, Tutik Sri; Permanasari, Adita Ayu; Adianti, Myrna; Aoki, Chie; Widyawaruyanti, Aty; Hafid, Achmad Fuad; Lusida, Maria Inge; Soetjipto,; Hotta, Hak

doi:10.1016/j.proche.2016.01.026

Cited by 3 publications

(5 citation statements)

References 5 publications

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“…The anti-HCV test was carried out following the previous test 5,6 . The sampels was dissolved in dimethyl sulfoxide (DMSO) to obtain stock solutions at a concentration of 100 mg/ml, stored at -20°C until it was used.…”

Section: Cells and Viruses Analysis Of Anti-hcv Activitiesmentioning

confidence: 99%

“…Cell staining was carried out following the previous test 5 . The Huh7it cells were prepared medium in 96-well plate (2.3 x 10 4 cell/mL) incubation 24 hours.…”

Section: Stainningmentioning

confidence: 99%

“…The cytotoxic test followed the previous test 5 . The cell viability of the samples was assessed by (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) (MTT) (Sigma-Aldrich) assay.…”

Section: Cytotoxic Assaymentioning

confidence: 99%

“…Mode of action assaytest is carried out by following the previous test 5,7 . These experiments were performed to assess the way of action of the samples.…”

Section: Cytotoxic Assaymentioning

confidence: 99%

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Faloak (Sterculia Quadrifida R.Br) Stem Bark Extract Inhibits Hepatitis C Virus JFH1

Dean¹,

Handajani²,

Khotib³

2019

Orient. J. Chem

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The discovery of antiviral drugs is developing new alternative medication and economics prices. One of the natural resources for alternative medicine is the water extract of faloak stem bark which is a material used by Kupang Community as an antiviral drug. The HCV JFH1 lifecycle inhibition is expressed in IC 50 (inhibitory concentration 50%) and the toxicity of water extract of faloak stem bark in hepatocyte cell line Huh7it is expressed in CC 50 (cytotoxicity concentration 50%). Determining the results obtained using Microsoft Office ® Excell 2013 ® . The water extract of faloak stem bark has an inhibition of HCV genotype 2a strain JFH1 with the IC 50 11.57 μg/mL and the toxicity water extract of faloak stem bark in the Huh7it cell line hepatocyte with the CC 50 >1000 μg/mL. Mode of action activities from water extract of faloak stem bark can be inhibited all step HCV life cycle. The first step is entry step has the inhibition 93.97%, post-entry step has the inhibition 96.75%, and combination step (entry and post-entry step) has the inhibition was 100%. The active compound in the water extract of faloak stem bark was epicatechin 875 mg/kg. The water extract of faloak stem bark can be a candidate for antivirals against HCV JFH1.

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Section: Cells and Viruses Analysis Of Anti-hcv Activitiesmentioning

confidence: 99%

“…Cell staining was carried out following the previous test 5 . The Huh7it cells were prepared medium in 96-well plate (2.3 x 10 4 cell/mL) incubation 24 hours.…”

Section: Stainningmentioning

confidence: 99%

Section: Cytotoxic Assaymentioning

confidence: 99%

“…Mode of action assaytest is carried out by following the previous test 5,7 . These experiments were performed to assess the way of action of the samples.…”

Section: Cytotoxic Assaymentioning

confidence: 99%

See 2 more Smart Citations

Faloak (Sterculia Quadrifida R.Br) Stem Bark Extract Inhibits Hepatitis C Virus JFH1

Dean¹,

Handajani²,

Khotib³

2019

Orient. J. Chem

View full text Add to dashboard Cite

show abstract

“…Some diseases caused by microbial infections include tuberculosis, typhus, diphtheria, cholera, dysentery, and pneumonia (Wagner et al, 2017). While viral infections can cause various diseases, like hepatitis C, it is also a worrying global problem (Emanuelli et al, 2014), infecting nearly 170 million people worldwide (Mustopa et al, 2012;Tumewu et al, 2016). Hepatitis C virus is a member of the genus Hepacivirus and the family Flaviviridae (Adianti et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and anti-HCV activity of triterpenoid and alkaloid compounds from Melochia umbellata (Houtt.) Stapf var Visenia (Paliasa)

2020

J app pharm sci

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Two triterpenoid and alkaloid compounds have been isolated, namely, 3-acetyl-12-en-28-oic acid and (R)-N-transferuloyloctopamine from Melochia umbellata (Houtt.) Stapf var Visenia. Both of these compounds were tested as an antimicrobial and anti-HCV activity. Isolation has been done by maceration, fractionation, and purification. The molecular structure was determined by IR spectroscopy and nuclear magnetic resonance (NMR) 1,2D ( 1 H-NMR, 13 C-NMR, HSQC, and HMBC). Antimicrobial assay of 3-acetyl-12-en-28-oic acid showed a zone of inhibition in the criteria of moderate to active against Escherichia coli (8.4 mm), Salmonella thypi (11.2 mm), Staphylococcus aureus (10.8 mm), and Candida albicans (8.5 mm), as well as compounds (R)-N-trans-feruloyloctopamine that is against E. coli (7.0 mm), S. thypi (10.55 mm), S. aureus (9.1 mm), and C. albicans (7.9 mm). Anti-HCV tests on the two compounds showed IC 50 values of 52.07 and 45.02 μg/ml, respectively. The results of these tests indicate that the two compounds are potential as an antibiotic candidate.

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Plant-Based Natural Products and Extracts: Potential Source to Develop New Antiviral Drug Candidates

et al. 2021

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Viral infections are among the most complex medical problems and have been a major threat to the economy and global health. Several epidemics and pandemics have occurred due to viruses, which has led to a significant increase in mortality and morbidity rates. Natural products have always been an inspiration and source for new drug development because of their various uses. Among all-natural sources, plant sources are the most dominant for the discovery of new therapeutic agents due to their chemical and structural diversity. Despite the traditional use and potential source for drug development, natural products have gained little attention from large pharmaceutical industries. Several plant extracts and isolated compounds have been extensively studied and explored for antiviral properties against different strains of viruses. In this review, we have compiled antiviral plant extracts and natural products isolated from plants reported since 2015.

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AntiHepatitis C Virus Activity of Alectryon serratus Leaves Extract

Cited by 3 publications

References 5 publications

Faloak (Sterculia Quadrifida R.Br) Stem Bark Extract Inhibits Hepatitis C Virus JFH1

Faloak (Sterculia Quadrifida R.Br) Stem Bark Extract Inhibits Hepatitis C Virus JFH1

Antimicrobial and anti-HCV activity of triterpenoid and alkaloid compounds from Melochia umbellata (Houtt.) Stapf var Visenia (Paliasa)

Plant-Based Natural Products and Extracts: Potential Source to Develop New Antiviral Drug Candidates

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