Antigenuria in visceral leishmaniasis: detection and partial characterisation of a carbohydrate antigen

Sarkari, Bahador; Chance, Michaël La; Hommel, M.

doi:10.1016/s0001-706x(02)00043-8

Cited by 70 publications

(55 citation statements)

References 25 publications

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“…In 1995, De Colmenares et al reported two polypeptide fractions of 72-75 kDa and 123 kDa in the urine of kala-azar patients [32]. In 2002, Sarkari et al described a urinary 5-20 kDa carbohydrate-based, heat stable antigen of VL patients [33]. A latex agglutination test (KAtex, Kalon Biological, UK) for detection of this antigen in urine samples was evaluated using samples from confirmed cases and controls from endemic and non-endemic regions.…”

Section: Antigen Detection In Urinementioning

confidence: 99%

Diagnosis of leishmaniasis

Elmahallawy

Martínez

Rodríguez‐Grangér

et al. 2014

J Infect Dev Ctries

142

119

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Leishmaniasis is a clinically heterogeneous syndrome caused by intracellular protozoan parasites of the genus Leishmania. The clinical spectrum of leishmaniasis encompasses subclinical ( not apparent), localized (skin lesion), and disseminated (cutaneous, mucocutaneous, and visceral) infection. This spectrum of manifestations depends on the immune status of the host, on the parasite, and on immunoinflammatory responses. Visceral leishmaniasis causes high morbidity and mortality in the developing world. Reliable laboratory methods become mandatory for accurate diagnosis, especially in immunocompromised patients such as those infected with HIV. In this article, we review the current state of the diagnostic tools for leishmaniasis, especially the serological test.

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Section: Antigen Detection In Urinementioning

confidence: 99%

Diagnosis of leishmaniasis

Elmahallawy

Martínez

Rodríguez‐Grangér

et al. 2014

J Infect Dev Ctries

142

119

View full text Add to dashboard Cite

show abstract

“…Antigen detection systems are also an ideal alternative to the antibody detection systems in immunocompromised patients and more particularly with the growing number of HIV coinfected cases, especially in advanced cases where the immune response is impaired (Rosenthal et al 1995). A latex agglutination test detecting a heat-stable, lowmolecular-weight carbohydrate antigen in the urine of VL patients has shown promising results (Attar et al 2001;Sarkari et al 2002). Several studies conducted in East Africa and the Indian subcontinent showed good specificity but only low to moderate (48-87 %) sensitivity (Rijal et al 2004;Sundar et al 2005;Sundar et al 2007).…”

Section: Discussionmentioning

confidence: 99%

ELISA and western blotting for the detection of Hsp70 and Hsp83 antigens of Leishmania donovani

Kaur

2012

J Parasit Dis

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“…Il est simple à collecter tout particulièrement chez les enfants. Il est également facile à conserver à température ambiante, présente peu de risque infectieux lors de la manipulation et permet de rechercher aussi bien les anticorps spécifiques, les antigènes solubles que l'ADN des agents parasitaires [19][20][21][22]. Son utilisation avec succès dans la mise en évidence d'anticorps spécifiques anti-rK39 et d'ADN leishmanien chez les enfants atteints de LV nous permet d'envisager l'utilisation de la salive pour un diagnostic sur le terrain [21].…”

Section: Leishmaniose Cutanéeunclassified

“…Son utilisation avec succès dans la mise en évidence d'anticorps spécifiques anti-rK39 et d'ADN leishmanien chez les enfants atteints de LV nous permet d'envisager l'utilisation de la salive pour un diagnostic sur le terrain [21]. La caractérisation précise des antigènes leishmaniens et leur nature glycoprotéique pourront ouvrir des perspectives dans la conception de nouveaux outils pour le diagnostic et le suivi des cas de LV [19,20].…”

Section: Leishmaniose Cutanéeunclassified