Antigens of the basement membrane and the peritumoral stroma in human colonic adenocarcinomas: An immunofluorescence study

Burtin, P; Chavanel, G.; Foidart, Jean‐Michel; Martin, E

doi:10.1002/ijc.2910300104

Cited by 86 publications

(20 citation statements)

References 16 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Conversely, almost all patients with CIV pattern II staining died by two years: 8 the first year with distant (5) or regional (2) metastasis or both (1); three more patients died the second At three years of evolution, the difference in survival as related to the CIV staining pattern was still highly significant (P<0.001) (Table II). For this statistical analysis, we used the Armitage-Cochran test (Table II) (Ingber et al, 1981), breast (Albrechtsen et al, 1981;Siegal et al, 1981), colon (Burtin et al, 1982;Forster et al, 1984), brain (McArdle et al, 1984), thyroid gland (Miettinen & Virtanen, 1984), melanomas (Natali et al, 1985) and laryngeal specimens (Visser et al, 1986).…”

Section: Resultsmentioning

confidence: 99%

“…Among them, type IV collagen (CIV) forms a network which is the architectural skeleton of basement membranes and laminin plays an important role in anchoring epithelial cells to CIV Terranova et al 1980;Timpl et al 1981). Other known basement membrane components are heparan sulphate-rich proteoglycan, entactin etc. Basement membranes are important in growth and differentiation of tissues, and constitute the first natural barrier to the invasion of cancerous cells (Ozzello, 1959;Siegal et al, 1981;Burtin et al, 1982;Liotta et al, 1983).…”

mentioning

confidence: 99%

“…Later, several groups studied basement membrane antigens in human tumours of different organs using immunohistological methods: Breast (Albrechtsen et al, 1981;Siegal et al, 1981), pancreatic gland (Ingber et al, 1981), colon (Burtin et al, 1982(Burtin et al, et 1983Forster et al, 1984), thyroid gland (Miettinen & Virtanen, 1984), brain (McArdle et al, 1984) melanomas (Natali et al, 1985) and recently laryngeal specimens (Visser et al, 1986), but no study has been reported on bladder tumours. Furthermore, Forster et al (1984) Immunoperoxidase method Antisera to CIV purified from the matrix of EHS sarcoma were raised in rabbits and the antibodies, which were kindly given us by J.M.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Collagen IV staining pattern in bladder carcinomas: relationship to prognosis

Daher¹,

Abourachid²,

Bove³

et al. 1987

Br J Cancer

View full text Add to dashboard Cite

A prospective study of type IV collagen in urothelial tissues was undertaken using an immunoperoxidase method on 125 ethanol fixed specimens. In normal and non cancerous urothelium, the basement membrane was continuously stained and the same pattern was seen in the 27 superficial carcinomas. In the 48 invasive bladder carcinomas, we observed two patterns of staining for collagen IV: in the first one, the staining line was conserved or partially fragmented (28 tumours), while in the second one the staining line was widely fragmented or absent in more than 5% of the tumour area (20 tumours). We found a highly significant statistical correlation between the pattern of staining and short term prognosis. Twenty-nine patients had an assessable follow-up of three years at least. All 16 patients with pattern I staining were alive at two years while only two out of 13 patients with pattern II staining survived two years (P less than 0.0001). At three years, all the patients with pattern II staining died while 11 patients with pattern I were still alive (P less than 0.001). These data provisionally indicate that the type IV collagen staining pattern may be of prognostic value in assessing the short term behaviour of invasive bladder carcinomas. It is thus logical to envisage that the treatment decisions may be influenced by the results of collagen IV staining. Images Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Collagen IV staining pattern in bladder carcinomas: relationship to prognosis

Daher¹,

Abourachid²,

Bove³

et al. 1987

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…Similar results were described by Siegal et al (1981) who stained for laminin and type IV collagen, again in breast carcinoma. In a study on human colonic adenocarcinomas Burtin et al (1982) also found marked alterations in basement membrane organisation of laminin and type IV collagen. However, no attempt was made in any of these studies to correlate loss of basement membrane staining for these adhesive glycoproteins and the subsequent development of metastases.…”

mentioning

confidence: 96%

Laminin and fibronectin in rectal adenocarcinoma: Relationship to tumour grade, stage and metastasis

Förster¹,

Talbot²,

Critchley³

1984

Br J Cancer

View full text Add to dashboard Cite

Summary Using an immunoperoxidase procedure, we have examined the distribution of laminin and fibronectin in normal human large intestinal mucosa and in 50 cases of rectal adenocarcinoma for which extensive clinical follow up was available. In normal tissue, laminin staining was largely restricted to basement membranes, including that underlying the epithelial cells, whereas fibronectin was found in both basement membranes and surrounding connective tissue.In rectal carcinomas, basement membrane-like staining for laminin associated with tumour cells was found in only 27 out of the 50 cases studied. Statistical analysis showed that the presence of laminin-containing basement membranes was correlated with low histological grade (well-differentiated tumours), but not with stage (progression through the bowel wall and the development of lymph node metastases) and, in a highly significant way, with a reduced incidence of distant metastases and increased patient survival.Although fibronectin was found in tumour cell basement membranes where these were present, it was also found in the stroma of all 50 tumours. There was no apparent correlation between the presence of stromal fibronectin and grade, stage or development of metastases.

show abstract

“…In various organs ECM composition has been found to be different which could be important in tumour cell-ECM adhesion (Nicolson, 1995). Additionally, some tumours, such as colon carcinomas, produce their own ECM components, and these components can modify peritumoural ECM composition (Burtin et al, 1983;Grigioni et al, 1986). In some tumour systems the expression of ECM components by tumour cells, such as the expression of oncofetal fibronectin (FN) by colorectal carcinomas, correlates with poor prognosis (Inufusa et al, 1995).…”

mentioning

confidence: 99%

Different adhesion properties of highly and poorly metastatic HT-29 colon carcinoma cells with extracellular matrix components: role of integrin expression and cytoskeletal components

1999

View full text Add to dashboard Cite

Summary Integrin-mediated tumour cell adhesion to extracellular matrix (ECM) components is an important step in the development of metastatic lesions. Thus, integrin expression and integrin-mediated adhesion of colon carcinoma cells to various ECM components was examined. Poorly (HT-29P) and highly (HT-29LMM) liver-metastatic colon carcinoma cells were used to study the rates of adhesion to collagen I (C I), collagen IV (C IV), laminin (LN), fibronectin (FN), or vitronectin (VN) in a static adhesion assay (10-120 min). Cells were untreated or treated with oligopeptides (RGD, GRGDS, YIGSR, RGES), anti-integrin antibodies, or colchicine, nocodazole, cycloheximide, acrylamide or cytochalasin D (to disrupt cytoskeletal structures). Both cell lines expressed similar patterns of integrin expression (α 2 , α 3 , α 6 , α v , β 1 , β 4 and β 5 ) by immunocytochemistry and immunoprecipitation. HT-29LMM cells showed significantly higher rates of adhesion to LN (P < 0.001) and FN (P < 0.001), but significantly poorer rates of adhesion to C I (P < 0.05) and C IV (P < 0.001) than HT-29P cells, respectively, adhesion to VN was insignificant. RGD and GRGDS inhibited HT-29LMM cell adhesion to FN only. Pretreatment with anti-β 1 or anti-α 2 integrin subunits suppressed adhesion to C I and C IV, and adhesion to LN was inhibited with anti-β 1 or anti-α 6 integrin. Anti-β 1 or anti-α v blocked adhesion to FN. Pretreatment of cells with cytochalasin D, cycloheximide or acrylamide inhibited adhesive interactions of both cell lines to the ECM components. In contrast, colchicine and nocodazole had no effect. The results demonstrate that adhesion of HT-29 cells to ECM is mediated, in part, by different integrins, depending on the substrate. Poorly and highly metastatic HT-29 cells possessed different patterns of adhesion to the various ECM substrates, but these differences were not due to different expression of integrin subunits. The results also suggested that the initial adhesion of poorly or highly metastatic HT-29 cells to ECM components requires, in part, the presence of native action and intermediate filaments, but not of microtubules. Thus the adhesion of tumour cells to ECM components may be dependent on signal transduction and assembly of microfilaments.

show abstract

Antigens of the basement membrane and the peritumoral stroma in human colonic adenocarcinomas: An immunofluorescence study

Cited by 86 publications

References 16 publications

Collagen IV staining pattern in bladder carcinomas: relationship to prognosis

Collagen IV staining pattern in bladder carcinomas: relationship to prognosis

Laminin and fibronectin in rectal adenocarcinoma: Relationship to tumour grade, stage and metastasis

Different adhesion properties of highly and poorly metastatic HT-29 colon carcinoma cells with extracellular matrix components: role of integrin expression and cytoskeletal components

Contact Info

Product

Resources

About