1988
DOI: 10.2307/3282489
|View full text |Cite
|
Sign up to set email alerts
|

Antigens of Cryptosporidium Sporozoites Recognized by Immune Sera of Infected Animals and Humans

Abstract: The humoral response of humans, calves, and horses to Cryptosporidium sporozoite antigens was evaluated using a western blot technique. Sera from calves, humans, and horses were obtained at various times following the detection of infection. Sera were reacted with detergent-solubilized, sodium dodecyl sulfate polyacrylamide gel-electrophoresed (SDS-PAGE) sporozoite antigens. The number of antigens recognized by immune sera from humans and animals increased with time postinfection. A 20-kDa antigen appears to b… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

10
76
2

Year Published

1989
1989
2012
2012

Publication Types

Select...
3
2
2

Relationship

0
7

Authors

Journals

citations
Cited by 111 publications
(88 citation statements)
references
References 26 publications
10
76
2
Order By: Relevance
“…While the mechanism of neutralization by MAb 3H2 has not yet been defined, its recognition of apical complex antigens, including CSL, and the surface pellicle of zoites suggests that it may target the attachment and/or invasion processes as well. The mechanism of neutralization by ␣P23 MAb 1E10 may involve interference with zoite locomotion, because P23 is deposited during gliding motility required in the invasion process (3,10,23). This type of sporozoan motility is characterized by attachment of zoite surface molecules to a substratum, posterior translocation, and forward locomotion (44,45).…”
Section: Discussionmentioning
confidence: 99%
See 3 more Smart Citations
“…While the mechanism of neutralization by MAb 3H2 has not yet been defined, its recognition of apical complex antigens, including CSL, and the surface pellicle of zoites suggests that it may target the attachment and/or invasion processes as well. The mechanism of neutralization by ␣P23 MAb 1E10 may involve interference with zoite locomotion, because P23 is deposited during gliding motility required in the invasion process (3,10,23). This type of sporozoan motility is characterized by attachment of zoite surface molecules to a substratum, posterior translocation, and forward locomotion (44,45).…”
Section: Discussionmentioning
confidence: 99%
“…GP25-200 was originally defined by MAb C4A1 as a sporozoite apical and surface pellicle glycoprotein complex comprised of multiple 25-to 200-kDa species identified in reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (1). P23, a 23-kDa sporozoite surface pellicle protein, was originally identified by MAb C6B6 (1,23). Passive immunization with MAbs C4A1 and C6B6 demonstrated that GP25-200 and P23 each expressed a neutralization-sensitive epitope; however, the efficacy observed was suboptimal (1,42).…”
mentioning
confidence: 99%
See 2 more Smart Citations
“…Because immunocompetent hosts usually recover spontaneously and prove refractive to reinfection, various immunological studies have been performed to identify parasite antigens using immune sera from naturally infected hosts. Immunoblot studies have resulted in the detection of a small molecular weight (MW) antigen (20 000-23 000 MW) in either intact oocyst or sporozoite life cycle stages which is frequently recognized by acute and convalescent sera from humans, goats, cattle and horses (1)(2)(3). However, radiolabelling of intact oocysts with '2^1 failed to locate this antigen on the exterior oocyst wall (2), whereas fluorescent-antibody studies indicated it may be located on the sporozoite and merozoite membranes (3).…”
Section: Introductionmentioning
confidence: 99%