Antigenotoxic Effect Against Ultraviolet Radiation‐induced <scp>DNA</scp> Damage of the Essential Oils from <i>Lippia</i> Species

Ruiz, Nathalia Quintero; Campo, Yuri Córdoba; Stashenko, Elena E.; Fuentes, Jorge Luı́s

doi:10.1111/php.12735

Cited by 22 publications

(7 citation statements)

References 66 publications

(75 reference statements)

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“…The oil from Lippia citriodora together with citral have been previously evaluated for their antigenotoxic effect against ultraviolet radiation-induced DNA damage using the SOS chromosome test, where they both showed antigenotoxicity [ 25 ]. Citral has shown diverse toxicity using different test systems.…”

Section: Resultsmentioning

confidence: 99%

Chemical Composition and Evaluation of the Biological Properties of the Essential Oil of the Dietary Phytochemical Lippia citriodora

et al. 2018

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The aim of the study was to characterize the chemical composition and biological properties of the essential oil from the plant Lippia citriodora grown in Greece. The essential oil volatiles were analyzed by gas chromatography–mass spectrometry GC-MS indicating citral as the major component. Τhe antimicrobial properties were assayed using the disk diffusion method and the minimum inhibitory and non-inhibitory concentration values were determined. Listeria monocytogenes, Staphylococcus epidermidis, Staphylococcus aureus, Saccharomyces cerevisiae, and Aspergillus niger were sensitive to Lippia citriodora oil, but not Escherichia coli, Salmonella Enteritidis, Salmonella typhimurium, and Pseudomonas fragi. Adversely, all microbes tested were sensitive to citral. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays were used to assess direct antioxidant activity, which proved to be weak for both agents, while comet assay was utilized to study the cytoprotective effects against H2O2-induced oxidative damage in Jurkat cells. Interestingly, the oil showed a more profound cytoprotective effect compared to citral. The antiproliferative activity was evaluated in a panel of cancer cell lines using the sulforhodamine B (SRB) and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-S-(phenylamino) carbonyl-2-tetrazolium hydroxide (XTT) assays and both agents demonstrated potent antiproliferative activity with citral being more cytotoxic than the oil. Taken together, the essential oil of Lippia citriodora and its major component, citral, exert diverse biological properties worthy of further investigation.

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Section: Resultsmentioning

confidence: 99%

Chemical Composition and Evaluation of the Biological Properties of the Essential Oil of the Dietary Phytochemical Lippia citriodora

et al. 2018

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“…Ionization radiation can cause DNA damage . After exposure to ionization radiation, γ‐H2AX, an important molecular marker of DNA damage, binds to DNA double‐strand break sites, recruits damage–repair proteins and forms γ‐H2AX foci .…”

Section: Discussionmentioning

confidence: 99%

Combination of RIZ1 Overexpression and Radiotherapy Contributes to Apoptosis and DNA Damage of HeLa and SiHa Cervical Cancer Cells

Yang

Xing

et al. 2018

Basic Clin Pharma Tox

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Although radiotherapy has been widely applied to treating cervical cancer in the clinic, its therapeutic efficacy is often restricted to the radioresistance of cancer cells. Retinoblastoma protein-interacting zinc finger gene 1 (RIZ1) has been suggested as a tumour suppressor gene, whereas its role in cervical cancer with or without radiotherapy has been unclear. In this study, two cervical cancer cell lines, HeLa and SiHa cells, stably transfected with RIZ1 overexpression plasmid were subjected to ionizing radiation, and their survival fractions were calculated by assessing their clonogenic abilities. Our results showed that the forced overexpression of RIZ1 significantly reduced the clonogenic survival rates of both HeLa and SiHa cells exposed to ionizing radiation. By analysing the cell apoptotic status, we found that the RIZ1-overexpressed cervical cancer cells under ionizing radiation were more vulnerable to damage, and more γ-H2AX foci were found in these cells. Furthermore, the volumes of tumour xenografts formed by the RIZ1-overexpressed cells in nude mice under ionizing radiation were smaller than those generated by the control cells. There were more morphological changes, apoptosis cells and lower expression of PCNA in RIZ1-overexpressed tumour tissues of mice after exposure to ionizing radiation. Taken together, our study demonstrates that the overexpression of RIZ1 combined with radiotherapy facilitates apoptosis and DNA damage of cervical cancer cells.

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“…Volatile secondary metabolites have been the subject of antigenotoxicity tests. L. alba, L. micromera and L. origanoides EOs were found antigenotoxic, and the evaluation of their main constituents showed that carvacrol, thymol, citral, p-cymene, and geraniol inhibited the UV-induced genotoxicity in the SOS chromotest [83].…”

Section: Antigenotoxicitymentioning

confidence: 99%

Study of Essential Oils Obtained from Tropical Plants Grown in Colombia

Stashenko¹,

Martı́nez²

2020

Essential Oils - Oils of Nature

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Researchers from several Colombian universities have joined efforts for over 15 years to characterize the composition and biological properties of more than a thousand samples of essential oils (EOs) obtained from aromatic plants collected during at least 30 botanical outings in different regions of Colombia. This chapter presents a brief description of essential oil extraction and chemical characterization techniques, followed by a representative list of references to publications on EO composition obtained from tropical aromatic plants that grow in Colombia. Opportunities for the development of interesting products for the pharmaceutical, cosmetics, hygiene, and food industries are illustrated with a few selected works on the evaluation of cytotoxicity, antioxidant, antiviral, antigenotoxic activities, and repellence of these essential oils.

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Antigenotoxic Effect Against Ultraviolet Radiation‐induced DNA Damage of the Essential Oils from Lippia Species

Cited by 22 publications

References 66 publications

Chemical Composition and Evaluation of the Biological Properties of the Essential Oil of the Dietary Phytochemical Lippia citriodora

Chemical Composition and Evaluation of the Biological Properties of the Essential Oil of the Dietary Phytochemical Lippia citriodora

Combination of RIZ1 Overexpression and Radiotherapy Contributes to Apoptosis and DNA Damage of HeLa and SiHa Cervical Cancer Cells

Study of Essential Oils Obtained from Tropical Plants Grown in Colombia

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