Antigenicity of the Measles Virus Haemagglutinin Studied by Using Synthetic Peptides

Mäkelä, Mika; Lund, Garry; Salmi, A.

doi:10.1099/0022-1317-70-3-603

Cited by 35 publications

(16 citation statements)

References 29 publications

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“…All 28 (Table III) affecting sites recognised currently as having important biological and immunological functions. These sites include the predicted B-cell epitope at aa309±318 of the H gene [Makela et al, 1989;Partidos, et al, 1991] and three antigenic sites at aa122±150, aa457±476, and aa519± 525 of the N gene [Buckland et al, 1989;Komase et al, 1990]. Amino acid 416 of the H gene, which is postulated to affect hemagglutination [Saito et al, 1995], had the same change in all three Ethiopia strains compared to the aa coded by the concensus nt sequence (Table III).…”

Section: Discussionmentioning

confidence: 89%

Measles virus strains circulating in Ethiopia in 1998–1999: Molecular characterisation using oral fluid samples and identification of a new genotype

Nigatu

Jin

Cohen

et al. 2001

Journal of Medical Virology

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A measles outbreak in December 1998 in Bedelle (vaccine coverage <40%) and two sporadic cases in Addis Ababa, Ethiopia, were investigated. Paired serum and oral fluid samples were collected 2-8 days after the onset of symptoms. A total of 53 of 55 outbreak cases and both sporadic cases were positive for serum measles virus-specific IgM. Oral fluid measles-specific IgM was positive in 71% of cases collected up to 5 days after onset and in 90% collected at 6-8 days. By contrast, 100% of oral fluid samples were positive for measles virus RNA by RT-PCR, suggesting that early collection of samples favoured the detection of measles virus RNA by RT-PCR. The measles virus strain in the outbreak was identified as genotype D4. One strain from a sporadic case was also genotype D4; the strain from the other sporadic case was assigned to clade D but was distinct. The degree of divergence from recognised clade D strains suggested that, together with three strains from the United Kingdom, it represents an additional genotype of clade D (GenBank accession numbers AF280800-280807).

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Section: Discussionmentioning

confidence: 89%

Measles virus strains circulating in Ethiopia in 1998–1999: Molecular characterisation using oral fluid samples and identification of a new genotype

Nigatu

Jin

Cohen

et al. 2001

Journal of Medical Virology

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“…This is demonstrated by the protection of passive immunization with measles hyperimmune sera [3] and of transplacentally acquired maternal antibodies [ 1.2], High-levels of neutralizing antibodies are concomitant with life-long immunity and persist in adults even decades after the infection. Several different epitopes have been identified using anti MV-H monoclonal antibodies [21][22][23][24][25][26]. In this study 61 overlapping !5-mer peptides covering the whole sequence of the MV-H protein were used to detect antibodies reacting with linear epitopes.…”

Section: Discussionmentioning

confidence: 96%

“…Makela et al, [26] have tested the reactivity of hutnan sera with ten 10-or 11-mer peptides covering about 16' K) of the MV-H protein sequence. As predicted using a Parker et at.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Neutralizing Antibody Response to the Measles Virus Using Synthetic Peptides of the Haemagglutinin Protein

Müller

Schroeder

et al. 1993

Scand J Immunol

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Infection or immunization with measles virus induces a protective immune reaction including neutralizing antibodies against the haemagglutinin and fusion protein. The reactivity of the polyclonal IgG response of sera obtained from late convalescent donors was studied, using overlapping 15mer peptides covering the complete sequence of the measles virus haemagglutinin. Most sera reacted with a similar set of peptides generating a characteristic binding pattern. The reactive peptides correspond to a region mediating cell hemolysis (aa310-325), to regions which serve as targets to neutralizing antibodies and to a putative transmembrane region (aa35-58). The latter region contains also a human T-cell epitope providing evidence of a non-random association of T- and B-cell epitopes. We also immunized different strains of mice and rabbits with measles virus. In contrast to the human sera, animal sera with strong neutralizing activities did not react with any of the H-protein peptides. The mostly weak reactivities with the linear sequences contrast with the strong neutralizing activities of the human or animal antibodies, suggesting that these primarily recognize the fusion protein or conformational epitopes of the haemagglutinin protein.

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“…In developing countries, one of the major difficulties is how to immunize infants who have maternal antibody against measles. For this purpose, a synthetic peptide vaccine is thought to be a candidate, and some laboratories have attempted to identify the B or T cell recognition epitopes of MV in order to develop an immunogenic peptide vaccine for measles [Beauverger et al, 1994;Verjans et al, 1995;Hummel and Bellini, 1995;Makela et al, 1989;Obeid et al, 1995]. Taylor et al [1991] described some different lineages of wild strains of MV co-circulating at a time, and Rota et al [1992Rota et al [ , 1994b reported that significant antigenic drift was observed in the H protein of MV and that recent isolates of MV were classified into significantly different lineages from those isolated previously.…”

Section: Introductionmentioning

confidence: 99%

Identification of three lineages of wild measles virus by nucleotide sequence analysis of N, P, M, F, and L genes in Japan

Yamaguchi¹

1997

J. Med. Virol.

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The nucleotide sequences of nucleocapsid (N), phosphoprotein (P), matrix (M), fusion (F), and large protein (L) genes were partly determined for 19 wild strains of measles virus (MV) isolated over the past 10 years in Japan (nucleotide position N: 1301-1700, P: 1751-2190, M: 3571-4057, F: 6621-7210, L: 10381-11133) and also for a MV strain obtained from a patient with subacute sclerosing panencephalitis (SSPE) who had natural measles in 1980. The phylogenetic trees of these strains drawn for respective genes were very similar to each other and revealed that all the wild strains were classified chronologically into 3 subgroups, those isolated in 1984, 1984-1989, and 1990-1994. The SSPE strain was classified into the subgroup of 1984. Phylogenetic tree analyses including other strains in the world revealed that Japanese strains in 1984 were classified into a distinct lineage which might correlate with the European strains from late 1970s to mid 1980s. Japanese strains from 1984 to 1989 were almost identical to those of the United States isolated from 1989 to 1992, and Japanese strains in 1990s were related closely to some of the MV strains isolated in 1994 in the United States. Genetic recombination among the MV genes seemed not to have occurred.

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Antigenicity of the Measles Virus Haemagglutinin Studied by Using Synthetic Peptides

Cited by 35 publications

References 29 publications

Measles virus strains circulating in Ethiopia in 1998–1999: Molecular characterisation using oral fluid samples and identification of a new genotype

Measles virus strains circulating in Ethiopia in 1998–1999: Molecular characterisation using oral fluid samples and identification of a new genotype

Analysis of the Neutralizing Antibody Response to the Measles Virus Using Synthetic Peptides of the Haemagglutinin Protein

Identification of three lineages of wild measles virus by nucleotide sequence analysis of N, P, M, F, and L genes in Japan

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