Antigenicity of polypeptides (poly-α-amino acids)—XVIII. Quantitative relationships among polymers containing glutamic acid, lysine and/or alannine rabbit antisera

Maurer, Paul H.; Pinchuck, Paul; Gerulat, Bernard F.

doi:10.1016/0019-2791(66)90178-9

Cited by 5 publications

(3 citation statements)

References 11 publications

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“…This procedure precipitated the maximum amount of antibody. The complete method for analysis of the antigen-antibody precipitates by the micro Folin technique is based on the previously described method (5). When more than 40 #g antibody N were precipitated, more concentrated reagents were used in analyses.…”

Section: Testing Of Sera--passive Cutaneous Anaphylaxis (Pca)mentioning

confidence: 99%

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Antigenicity of Polypeptides (Poly Alpha Amino Acids)

Maurer

Gerulat

Pinchuck

1964

The Journal of Experimental Medicine

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Previous reports from this laboratory have dealt with observations on the immunogenicity in rabbits (1-5), guinea pigs (6, 7), and man (8) of synthetic random copolymers consisting of 2 and 3 L-a-amino acids. In the rabbit studies, the copolymers consisting of the amino acids, glutamic acid and alanine, in a ratio of 60:40 (G60A40) t and the terpolymer consisting of the latter amino acids and lysine (G~L~sAs0) were very effective antigens and have repeatedly been so in our laboratory. This latter terpolymer has also been found to be highly antigenic in guinea pigs (7), man (8), and mice (9). The experiments presented in this report on the immunogenicity of a new group of polymers were undertaken to learn: (a) whether polymers consisting of other ratios of glutamic acid and alanine or glutamic acid and other amino acids are effective antigens; (b) the possible basis for the enhanced immunogenicity of G4~asA,0. When negative or poor responses were noted with the polymers in rabbits, the material was then tested in guinea pigs. This report will present the findings on the immunogenicity of the preparations and a subsequent publication will present the quantitative relationships among the various polymers and their antisera. Materials and MethodsMaterials.- Table I lists the random polymers studied. The methods employed in preparing the polymers from the N-carboxy a-amino acid anhydrides, their characterization and the techniques *

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Section: Testing Of Sera--passive Cutaneous Anaphylaxis (Pca)mentioning

confidence: 99%

“…for immunizing rabbits and guinea pigs have been referred to in the previous publications (1)(2)(3)(4)(5)(6)(7)(8).…”

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confidence: 99%

Antigenicity of Polypeptides (Poly Alpha Amino Acids)

Maurer

Gerulat

Pinchuck

1964

The Journal of Experimental Medicine

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“…Recent work with synthetic polypepfide antigens has shown that such materials can be antigenlcally simple (1). It has been demonstrated that the ability of poly-T.lysine to act as a hapten carrier for the 2,4-dinitrophenyl group in guinea pigs is transmitted as a simple Mendefian determinant (2)(3)(4)(5).…”

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Antigenicity of Polypeptides (Poly Alpha Amino Acids)

Pinchuck

Maurer

1965

The Journal of Experimental Medicine

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The ability of mice to form antibodies against the random terpolymer glu57lys38ala5 is controlled by a codominant Mendelian factor. Three of 7 inbred strains were 100 per cent responders; the others were completely negative. All of these strains could make antibody to related polymers with higher alanine content (10 and 40 mole per cent). Breeding studies using the progeny of Swiss mice indicated that a similar genetic factor was involved.

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Synthesis of two sequential polypeptides by dispersion in benzene and their circular dichroism spectra in aqueous solution: Poly(L‐Glu‐L‐Lys‐L‐Ala‐Gly) and Poly(L‐Ala‐D‐Glu‐L‐Lys‐D‐Ala‐Gly)

1973

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SynopsisThe monomers ~-benzylglutamyl-s-benzyloxycarbonyl-lysylalanylglycine pentachlorophenyl ester and alanyl-r-benzyl-D-glutamyl-e-ben~yloxycarbonyllysyl-~-alanylglycine pentachlorophenyl ester, were polymerized in dilute solutions of dimethylformamide (DMF) or as dispersions in the same volume of benzene. After deprotection with hydrogen bromide, the products were either chromatographed on Sephadex G-50 or dialyzed. The polymers derived from the polymerization in benzene were considerably larger than those from DMF. The results in benzene indicated that high monomer to solvent ratios are not necessary for the production of high-molecular-weight sequential polypeptides. Circular dichroism spectra of the polymers and monomers at neutral and acid pH indicated that poly (cGlu-LLys-cAla-Gly) exists in a random coil I configuration and poly(cAla-D-Glu-cLys-D-Ala-Gly) exists in a p conformation.

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Antigenicity of polypeptides (poly-α-amino acids)—XVIII. Quantitative relationships among polymers containing glutamic acid, lysine and/or alannine rabbit antisera

Cited by 5 publications

References 11 publications

Antigenicity of Polypeptides (Poly Alpha Amino Acids)

Antigenicity of Polypeptides (Poly Alpha Amino Acids)

Antigenicity of Polypeptides (Poly Alpha Amino Acids)

Synthesis of two sequential polypeptides by dispersion in benzene and their circular dichroism spectra in aqueous solution: Poly(L‐Glu‐L‐Lys‐L‐Ala‐Gly) and Poly(L‐Ala‐D‐Glu‐L‐Lys‐D‐Ala‐Gly)

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