Antigenicity of Polypeptides (Poly Alpha Amino Acids)

“…Many compounds of low molecular weight are nonimmunogenic or are immunogenic only when coupled to a larger molecule. However, the immunogenicity of compounds of tool wt of approximately 5000 or below has been reported (19)(20)(21)(22)(23)(24). Two such compounds, ce,N-DNP-hepta-L-Lysine, and a p-azo-benzenearsonate conjugate of hexa-L-tyrosine, which were found to be immunogenic (22,23), have approximately the same molecular weight as that of the decapeptide which we found was not immunogenic although it does combine with anti-TMVP.…”

Section: Discussionmentioning

confidence: 99%

Studies on the Immune Response to a Characterized Antigenic Determinant of the Tobacco Mosaic Virus Protein

S̄pitler

Benjamini

Young

et al. 1970

The Journal of Experimental Medicine

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The following peptides have previously been shown to bind specifically with antibodies to TMVP: (a) An eicosapeptide representing residues 93–112 of TMVP and having the sequence Ileu-Ileu-Glu-Val-Glu-AspNH2-GluNH2-Ala-AspNH2-Pro-Thr-Thr-Ala-Glu-Thr-Leu-Asp-Ala-Thr-Arg. (b) Its C-terminal decapeptide. (c) Its C-terminal pentapeptide. (d) N-octanoyl-C-terminal-tripeptide. (e) (Lys)4-C-terminal-pentapeptide. (f) (Lys)7 C-terminal-pentapeptide. The present communication deals with the investigation of several parameters of the immunological activity of the peptides. The results show that none of the peptides tested were immunogenic in guinea pigs, nor did they stimulate the incorporation of 14C-thymidine by spleen cells derived from TMVP-primed animals. Results also showed that all of the peptides tested could elicit specific delayed and immediate skin reactions in TMVP-sensitized guinea pigs, and furthermore, that the peptides could specifically inhibit the migration of peritoneal exudate cells derived from these animals. The elicitation of delayed skin reactions and the ability to inhibit migration of peritoneal exudate cells were independent of carrier specificity.

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“…their synthesis and characterization have been previously described (9)(10)(11)(12). E-DNP-lysine, and di-O,N-DNP-L-tyrosine were purchased from ICN Nutritional Biochemicals Div., International Chemical & Nuclear Corp., Cleveland, Ohio.…”

Section: Abbreviationmentioning

confidence: 99%

The specificity of cellular immune responses II. The structure of antigenic determinants leading to T-lymphocyte stimulation.

Janeway¹,

Maurer²,

Dailey³

et al. 1976

The Journal of Experimental Medicine

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In a previous report (1), guinea pigs primed with the hapten 2,4-dinitrophenyl (DNP) coupled directly to mycobacteria were shown to possess thymus-derived (T) lymphocytes responsive to DNP on a wide variety of carriers. This system is of interest for several reasons. First, it contrasts with most T-cell responses to hapten-carrier conjugates, which are specific for carrier or for specific conjugate determinants, but not for the same hapten on heterologous carriers (2). Second, it allows the comparison of responses of T lymphocytes to those of thymusindependent, bone-marrow-derived (B) lymphocytes to the same, chemically defined determinant. Finally, it permits the precise analysis of the structure of antigenic determinants recognized by such T cells; since it is necessary that the determinant contain the hapten for T-cell responses to occur, the hapten becomes a marker for the determinant. If the specificity of such T-cell responses could be shown to resemble closely the specificity of B-cell responses to DNP, and of anti-DNP antibody, then a strong argument could be made for the identity of the specific receptors for antigen on T and B lymphocytes; Blymphocyte receptors are generally believed to be immunoglobulin (Ig) in nature (2-4). On the other hand a clear difference in the specificity ofT-lymphocyte responses compared to B-lymphocyte responses might imply that these two types of lymphocytes use entirely different molecules to perform a similar function, namely specific antigen recognition.Previous studies (1) of guinea pigs immunized with DNP-mycobacteria have shown that the in vitro proliferative response of a purified population of guinea pig T lymphocytes to DNP conjugates is independent of the few B cells in the system, and is indeed a T-cell response. The determinant recognized involves both hapten and carrier, since neither alone can stimulate such T cells. The

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Antigenicity of Polypeptides (Poly Alpha Amino Acids)

Cited by 26 publications

References 23 publications

Poly-L-glutamate, an anionic polymer, enhances transgene expression for plasmids delivered by intramuscular injection with in vivo electroporation

Poly-L-glutamate, an anionic polymer, enhances transgene expression for plasmids delivered by intramuscular injection with in vivo electroporation

Studies on the Immune Response to a Characterized Antigenic Determinant of the Tobacco Mosaic Virus Protein

The specificity of cellular immune responses II. The structure of antigenic determinants leading to T-lymphocyte stimulation.

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