Antigenicity of Cryopreserved Corneas

Bourne, William M.

doi:10.1001/archopht.1975.01010020223011

Cited by 5 publications

(1 citation statement)

References 18 publications

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“…This finding supported results of earlier animal studies of Bourne (Bourne 1975), suggesting that there was no significant effect of cryopreservation on the antigenicity of corneal tissue.…”

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confidence: 91%

HLA antigens in lyophilised corneal lenticules

Tabandeh

Morris

Rostron

et al. 1998

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To investigate the presence of Human Leukocyte Antigens (HLA) in organ cultured corneas, in lyophilised epikeratophakia lenticules prepared by dry state lathing, and in lenticules subjected to prolonged rehydration in Balanced Salt Solution (BSS). Methods: Twelve lenticules and 3 organ cultured corneas were studied for the presence of HLA-ABC (class I), and HLA-DR, -DQ, and -DP (class II), antigens using the immunoperoxidase technique. Results: HLA-ABC antigens were detected in all lenticules. HLA-DR antigen was also detected in low concentrations in 6 out of 12 lenticules. HLA-DQ and -DP antigens were generally absent. Incubation in BSS for 24 and 48 hours did not change the expression of antigens. Conclusions: HLA-class I, and to some degree HLA-class II antigens, are present in the stroma of both organ cultured corneas, and in lyophilised lenticules. Incubation in BSS for up to 48 hours did not reduce the expression of antigens.

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confidence: 91%