Antigenic structure of human tumour necrosis factor: Recognition of distinct regions of TNFα by different tumour cell receptors

Rathjen, Deborah A.; Cowan, Keng; Furphy, Louise J.; Aston, R.

doi:10.1016/0161-5890(91)90089-3

Cited by 21 publications

(13 citation statements)

References 24 publications

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“…TNF-␣ is the critical stimulating factor in regulating choriodecidual IL-1␤, IL-10, and PGE 2 production, although it should be noted that neutralization of TNF-␣ did not result in a complete inhibition of LPS-stimulated cytokine and PG production. The concentrations of the Abs used were chosen based on the manufacturer's recommendations (34,35) and confirmed by removal of immunodetectable TNF-␣ in preliminary experiments. However, the lack of complete inhibition could reflect inadequate penetration of the neutralizing Abs as they were added concurrently with the LPS treatment.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Critical Paracrine Interactions Between TNF-α and IL-10 Regulate Lipopolysaccharide-Stimulated Human Choriodecidual Cytokine and Prostaglandin E2 Production

Sato

Keelan

Mitchell

2003

The Journal of Immunology

104

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Increased production of PGs by gestational membranes is believed to be a principal initiator of term and preterm labor. Intrauterine infection is associated with an inflammatory response in the choriodecidua characterized by elevated production of cytokines and PGs. The precise physiological significance of enhanced choriodecidual cytokine production in the mechanism of preterm labor remains uncertain. These studies were undertaken to dissect the roles and regulation of endogenous cytokines in regulating PG production by human choriodecidua. We used LPS treatment of human choriodecidual explants as our model system. In choriodecidual explant cultures, LPS (5 μg/ml) induced a rapid increase in TNF-α production, peaking at 4 h. In contrast, IL-10, IL-1β, and PGE2 production rates peaked 8, 12, and 24 h, respectively, after LPS stimulation. Immunoneutralization studies indicated that TNF-α was a primary regulator of IL-1β, IL-10, and PGE2 production, while IL-1β stimulated only PGE2 production. Neutralization of endogenous IL-10 resulted in increased TNF-α and PGE2 production. IL-10 treatment markedly decreased TNF-α and IL-1β production, but had no effect on PGE2 production. Taken together, these results demonstrate that the effects of LPS on choriodecidual cytokine and PG production are modulated by both positive and negative feedback loops. In the setting of an infection of the intrauterine, TNF-α may be a potential target for treatment intervention; IL-10 could be one such therapeutic.

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Section: Discussionmentioning

confidence: 99%

“…The explants were pretreated with the IL-1Ra for 1 h before the addition of LPS and supernatants were harvested 24 h later. The concentrations selected were chosen according to manufacturer's specifications for neutralizing the cytokines (34,35).…”

Section: Immunoneutralization Experimentsmentioning

confidence: 99%

Critical Paracrine Interactions Between TNF-α and IL-10 Regulate Lipopolysaccharide-Stimulated Human Choriodecidual Cytokine and Prostaglandin E2 Production

Sato

Keelan

Mitchell

2003

The Journal of Immunology

104

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“…TNF-neutralizing mAb (mAb 54) against human TNF was prepared as described previously (29). The following mAbs were purchased: anti-human intercellular adhesion molecule 1 (ICAM-1) (Immunotech, Marseille, France), anti-human endothelial leukocyte adhesion molecule 1 (ELAM-1) (E-selectin) and vascular cell adhesion molecule 1 (VCAM-1) (Becton Dickinson and Co., San Jose, CA), and horseradish peroxidase-conjugated rabbit immunoglobulin (Dakopatts, Copenhagen, Denmark) anti-CD (18).…”

Section: Antibodiesmentioning

confidence: 99%

A synthetic tumor necrosis factor-alpha agonist peptide enhances human polymorphonuclear leukocyte-mediated killing of Plasmodium falciparum in vitro and suppresses Plasmodium chabaudi infection in mice.

Kumaratilake

Rathjen²,

Mack³

et al. 1995

J. Clin. Invest.

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“…One portion ofthe IgG was used as the capture antibody and another portion of the same IgG was biotinylated as described below and used as the tracer antibody. A panel of murine monoclonal antibodies was prepared against recombinant human TNF-a and their epitope specificities were mapped as described by Rathjen et al (1991). A cocktail of antibodies directed against two non-overlapping domains of the TNF-a molecule were used for the ELISA, one set forcaptureand the other set was biotinylated and used as the tracer.…”

Section: Elisas For Il-l and Tnf-amentioning

confidence: 99%

Effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-2, interferon-gamma (IFN-γ), tumour necrosis factor-alpha (TNF-α) and IL-6 on the production of immunoreactive IL-1 and TNF-α by human monocytes

Danis

Franic

Rathjen

et al. 1991

Clinical and Experimental Immunology

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SUMMARYThe effects of GM-CSF, IL-2, IFN-y. TNF-a and IL-6 on the production of IL-I (both secreted and cell associated) and TNF-a by peripheral blood monocytes were studied. Monocytes were cultured for 20 h in suspension and in serum-free conditions which minimized background stimulation of monokine production. GM-CSF. IL-2 and TNF-a directly induced the production of cell-associated IL-1 but liUlcornoIL-l or TNF-a secretion. Combination of GM-CSF with !FN-y. IL-2 or TNF-a synergistically enhanced IL-1 secretion and had an additive eflect on cell-associated IL-1 production. Combination of IL-2 with IFN-7 or TNF-a also synergistically enhanced IL-I secretion but theefiect on cell-associated IL-I production was less than additive. GM-CSF synergistically enhaneed TNF-a secretion induced by IFN-y but not by lipopolysaccharide. GM-CSF did not enhanee TNF-a secretion induced by IL-2 or TNF-a. In contrast. IL-2 syncrgisticatly enhanced TNF-a seeretion induced by IFN--/. These results are discussed in relation lo cytokine involvement in rheumatoid arthritis.

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Antigenic structure of human tumour necrosis factor: Recognition of distinct regions of TNFα by different tumour cell receptors

Cited by 21 publications

References 24 publications

Critical Paracrine Interactions Between TNF-α and IL-10 Regulate Lipopolysaccharide-Stimulated Human Choriodecidual Cytokine and Prostaglandin E2 Production

Critical Paracrine Interactions Between TNF-α and IL-10 Regulate Lipopolysaccharide-Stimulated Human Choriodecidual Cytokine and Prostaglandin E2 Production

A synthetic tumor necrosis factor-alpha agonist peptide enhances human polymorphonuclear leukocyte-mediated killing of Plasmodium falciparum in vitro and suppresses Plasmodium chabaudi infection in mice.

Effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-2, interferon-gamma (IFN-γ), tumour necrosis factor-alpha (TNF-α) and IL-6 on the production of immunoreactive IL-1 and TNF-α by human monocytes

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