Antigenic Relationship among the Eight Prototype and New Serotype Strains of <i>Orientia tsutsugamushi</i> Revealed by Monoclonal Antibodies

Kang, Jae-Seung; Chang, William

doi:10.1111/j.1348-0421.1999.tb02397.x

Cited by 14 publications

(14 citation statements)

References 25 publications

(37 reference statements)

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“…These strains are antigenically different but could not be differentiated by our method. 18,19 Only the Boryong strain, and not the Kuroki strain, is mentioned in this study, because it is a long-term predominant strain and there has been no evidence of divergence. The term Kanda/Kawasaki is used for the 14 cases of Kanda or Kawasaki strains.…”

Section: Genotypes Of O Tsutsugamushimentioning

confidence: 99%

Antigenic Drift of Orientia tsutsugamushi in South Korea as Identified by the Sequence Analysis of a 56-kDa Protein-Encoding Gene

Park¹,

Lee²,

Kwak³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Abstract. This study was performed to determine the nationwide antigenic diversity of Orientia tsutsugamushi in South Korea. Sequence analysis was performed around variable domains I and II of a 56-kDa protein-encoding gene. We used eschar to overcome the disadvantages of conventional serotyping. The serological passive hemagglutination assay (PHA) was assessed based on the genotyping results. We analyzed 153 isolates from scrub typhus patients in major endemic areas and found that Boryong was the major strain (68.6%). New strains were also identified: Taguchi (19.6%), Kanda/Kawasaki (9.2%), and UAP7 (1.3%). PHA yielded significantly fewer positive results among Kawasaki strains ( P < 0.001), which are not included in the PHA antigen panel. In South Korea, Boryong was still the predominant strain, but the sequence analysis identified new changes in minor strains (30.1%). This antigenic drift had a negative effect on the PHA results. Periodic surveillance of the contemporary strains using sequence analysis is needed.

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Section: Genotypes Of O Tsutsugamushimentioning

confidence: 99%

Antigenic Drift of Orientia tsutsugamushi in South Korea as Identified by the Sequence Analysis of a 56-kDa Protein-Encoding Gene

Park¹,

Lee²,

Kwak³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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“…Diagnostic treatment must be started sometimes even before the definite diagnosis is confirmed by the results of laboratory examinations. The immunity after Tsutsugamushi disease is knownto be serotype specific, so the sameperson can be infected with a different serotype strain during the convalescent period (6). Therefore, it is very important to know the prevalent serotype in a local area for serodiagnosis and epidemiology.…”

Section: Discussionmentioning

confidence: 99%

Successful Diagnosis Using Scab for PCR Specimen in Tsutsugamushi Disease.

Ono

Higuchi

et al. 2002

Intern. Med.

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Wetreated a case of Tsutsugamushi disease diagnosed by polymerase chain reaction (PCR) using a scab specimen at the bite site of trombiculid mites. Otherwise the diagnosis could not be confirmed by serum antibody test nor the PCRtest of blood. The genome of Rickettsia tsutsugamushi was detected and identified as the Kawasaki serotype strain. Anattempt to extract the genomefrom the scab has not been reported, thus our data suggest that the scab is a useful specimen to confirm the diagnosis of Tsutsugamushi disease. (Internal Medicine 41: 408-411, 2002)

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“…The methods for the production of hybridomas and ascitic fluid were as described previously (9). MAb FS15 and M686-8 reacted with the 56-kDa and 46-kDa proteins, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Previously, a panel of 36 MAbs that were raised against O. tsutsugamushi has been described (9). The MAbs showed variable reactivity to the eight prototype strains …”

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confidence: 99%

Characterization of Monoclonal Antibody Reacting Exclusively against Intracellular Orientia tsutsugamushi

Mee-Kyung

Odgerel

Chung

et al. 2002

Microbiology and Immunology

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Intracellular bacteria often change the expression of their genes in order to adapt to new environmental conditions. Here we describe a monoclonal antibody (MAb) that reacts exclusively against intracellular Orientia tsutsugamushi Although MAb applied to the 56‐kDa protein, a major outer membrane protein, reacted against a large number of bacteria that had attached to host cells at the early stage of infection, M686–13 reacted against only a minor portion of the attached bacteria. In the later stage of the intracellular growth cycle, both antibodies showed identical staining patterns by double immunofluorescent staining. These results suggest that M686–13 reacted to an epitope or a protein that had probably been expressed during the intracellular growth cycle and rapidly diluted or degraded upon release into the extracellular environment. Although its molecular characteristics remain unknown, the reactive antigen may prove to be a novel developmental antigen and this MAb could be used as reagent for the staining of viable O. tsutsugamushi.

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Antigenic Relationship among the Eight Prototype and New Serotype Strains of Orientia tsutsugamushi Revealed by Monoclonal Antibodies

Cited by 14 publications

References 25 publications

Antigenic Drift of Orientia tsutsugamushi in South Korea as Identified by the Sequence Analysis of a 56-kDa Protein-Encoding Gene

Antigenic Drift of Orientia tsutsugamushi in South Korea as Identified by the Sequence Analysis of a 56-kDa Protein-Encoding Gene

Successful Diagnosis Using Scab for PCR Specimen in Tsutsugamushi Disease.

Characterization of Monoclonal Antibody Reacting Exclusively against Intracellular Orientia tsutsugamushi

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