Antigenic Proteins Associated with Calcareous Corpuscules of Taenia solium: Partial Characterization of a Calcium-Binding Protein

Zurabián, Rimma; Carrero, Julio César; Rodriguez‐Contreras, Dayana; Willms, Kaethe; Laclette, Juan Pedro

doi:10.1016/j.arcmed.2004.10.001

Cited by 10 publications

(11 citation statements)

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“…Previous studies have demonstrated that TsM CC bound to several molecules and suggested that CC might be involved in trafficking of those proteins [ 20 – 23 ]. We analyzed the protein ligands of CC with CF or cytosolic proteins extracted from SN.…”

Section: Resultsmentioning

confidence: 99%

“…Most of these ligands shared common properties of calcium dependency [ 35 – 37 ] and/or post-translationally modified by glycosylation [ 8 ]. Previous studies have reported that cestode CC bound to calcium binding proteins [ 20 , 23 ] and glycoproteins [ 21 , 23 ]. Calcium-related biochemical properties of TsMFas1 and TsMFas2 proteins were not examined in this study.…”

Section: Discussionmentioning

confidence: 99%

“…CC is thought to be involved in the focal accumulation of ions and heavy metals, osmoregulation and protection of parasites against calcification [ 19 ]. CC has binding affinity to glycoproteins and calcium-binding proteins [ 20 – 23 ]. A recent study with Echinococcus granulosus metacestode has demonstrated that CC might undergo active catabolic process during drug-induced autophagy [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Fasciclin-calcareous corpuscle binary complex mediated protein-protein interactions in Taenia solium metacestode

et al. 2017

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BackgroundNeurocysticercosis (NC) caused by Taenia solium metacestode (TsM) is a serious neurological disease of global concern. Diverse bioactive molecules involved in the long-term survival of TsM might contribute to disease progression. Fasciclin (Fas) is an extracellular protein that mediates adhesion, migration and differentiation of cells by interacting with other molecules. We hypothesized that TsMFas might bind to calcareous corpuscle (CC) through its adhesive property and participate in crucial protein-protein interactions, thus contributing to the creation of a symbiotic interactome network.MethodsTwo paralogous TsMFas (TsMFas1 and TsMFas2) were isolated, and their molecular properties were characterized. The co-localization pattern of TsMFas1 and TsMFas2 with CC was determined. CC-TsMFas binary complex was generated by incubating CC with recombinant proteins (rTsMFas1 and 2). In vitro binding assay of CC-rTsMFas1 or CC-rTsMFas2 binary complex with TsM cellular proteins extracted from scolex and neck was conducted. Their binding partners were identified through proteomic analysis. Integrated protein-protein interaction networks were established.Results TsMFas1 (6072 bp long) was composed of 15 exons (841 amino acid polypeptide) interrupted by 14 introns. TsMFas2 (5201 bp long) comprised of 11 exons (597 amino acids) and 10 intervening introns. These proteins displayed 22% amino acid sequence identity to each other, but tightly conserved Fas-related domains. Several isoforms of Fas1 and Fas2 proteins might have been expressed through post-translational modifications. They showed adhesion activity with other cells. TsMFas proteins were largely distributed in parenchymal regions of the scolex and bladder wall. These molecules were co-localized with CC, a unique organelle found in platyhelminths. Subsequent proteome analysis of CC-Fas binary complex mediated protein-protein interactions revealed seven protein ligands in the TsM cellular proteins. Their functions were mainly segregated into carbohydrate metabolism (enolase, phosphoenolpyruvate carboxykinase, phosphoglycerate kinase and glyceraldehyde 3-phosphate dehydrogenase) and cytoskeleton/cellular motility (actin, paramyosin and innexin nuc-9). Those proteins had direct (physical) and/or indirect (functional) relationships along with their biochemical properties and biological roles.ConclusionProtein repertoires strongly suggest that TsMFas and CC may symbiotically mediate protein-protein interactions during biological processes to maintain efficacious homeostatic functions and ensure the prolonged survival of TsM in the host.Electronic supplementary materialThe online version of this article (10.1186/s13071-017-2359-2) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fasciclin-calcareous corpuscle binary complex mediated protein-protein interactions in Taenia solium metacestode

et al. 2017

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“…They form in the lumen of the protonephridial ducts and may be involved in osmoregulation [61,62]. Recent experimental evidence, has demonstrated, that CC contain a calcium-binding protein of high molecular weight, which is homologous to the calciumbinding protein of E. granulosus, and appears to be a relevant component of the organic matrix of CC [63].…”

Section: The Adult Tapewormmentioning

confidence: 99%

Morphology and Biochemistry of the Pork Tapeworm, Taenia solium

Willms

2008

CTMC

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This chapter describes the life cycle, general morphology and ultrastructure of the larval and adult stages of Taenia solium, a parasitic flatworm of humans found in underdeveloped countries. Experimental results describing the role of proteins and glycoproteins in the host-parasite relationship, as well as the various strategies the larval stage has developed to evade the host immune responses are analyzed. Characteristics of the tapeworm attachment site in the hamster intestine and the host inflammatory reaction are reviewed. The general morphology and ultrastructure of the experimental tapeworm is described, with emphasis on muscle fiber distribution, the abundance of cytoplasmic glycogen and its association with gap junctions, the development of testis, structure of mature spermatids and vas efferens. Recent descriptions of T. solium actin, myosin and calreticulin components, metabolic steroid pathways, apoptosis and glucose uptake of tapeworms in the hamster model are reviewed.

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“…12). Em concordância com nossos resultados, tanto ARAUJO 2008 tanto para a purificação de extratos parasitários PERALTA et al, 2010), quanto para detecção de antígenos de cisticerco de T. solium em LCR de pacientes acometidos por essa patologia (ESPÍNDOLA et al, 2002), bem como para caracterização antigênica (ZURABIAN et al, 2005). antigenicidade e especificidade para o diagnóstico da NC (ESPINDOLA et al, 2002(ESPINDOLA et al, , 2005.…”

Section: Antígeno Recombinanteunclassified

Obtenção de proteína recombinante baseada em antígenos do líquido vesicular de Taenia crassiceps: aplicação no imunodiagnóstico da neurocisticercose

Gomes¹

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Antigenic Proteins Associated with Calcareous Corpuscules of Taenia solium: Partial Characterization of a Calcium-Binding Protein

Cited by 10 publications

References 30 publications

Fasciclin-calcareous corpuscle binary complex mediated protein-protein interactions in Taenia solium metacestode

Fasciclin-calcareous corpuscle binary complex mediated protein-protein interactions in Taenia solium metacestode

Morphology and Biochemistry of the Pork Tapeworm, Taenia solium

Obtenção de proteína recombinante baseada em antígenos do líquido vesicular de Taenia crassiceps: aplicação no imunodiagnóstico da neurocisticercose

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