Antigenic properties of VP15 from white spot syndrome virus in kuruma shrimp Marsupenaeus japonicus

Boonyakida, Jirayu; Xu, Jian; Satoh, Jun; Nakanishi, Tetsu; Mekata, Toru; Kato, Tatsuya; Park, Enoch Y.

doi:10.1016/j.fsi.2020.03.061

Cited by 18 publications

(17 citation statements)

References 45 publications

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“…In our previous study, we found that VP15, when administered to Kuruma shrimp IM at a dose of 0.04 mg/g shrimp using the prime-and-boost method, increased the survival rate to 78% at 20 dpi. Moreover, the same GST-VP15 revealed a different result, in which the final survival rate was approximately 43% with a dose of 0.01 mg/g shrimp using the same protocol 38 . This difference can be explained by the amount of the recombinant protein delivered to the shrimp.…”

Section: Discussionmentioning

confidence: 92%

“…These viral subunits were confirmed to improve the survival rate after challenging shrimp with WSSV. Recently, we reported that VP15 can also provide a protective effect in Kuruma shrimp ( M. japonicus ) against WSSV after immune prime-and-boost via intramuscular injection 38 .…”

Section: Introductionmentioning

confidence: 99%

“…No protein crystal structure has been reported yet, and only one trial used VP15-based material (DNA vaccine encoding VP15 42 ) for immunizing shrimp against WSSV. We have previously demonstrated that VP15 can enhance shrimp survivability after challenge with WSSV 38 . In this study, we attempted to determine the antigenic domain of the VP15 protein by in vivo animal experiments with M. japonicus using a purified truncated VP15 series and synthetic peptides derived from VP15 at the peptide level.…”

Section: Introductionmentioning

confidence: 99%

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Identification of antigenic domains and peptides from VP15 of white spot syndrome virus and their antiviral effects in Marsupenaeus japonicus

Boonyakida

Satoh

et al. 2021

Sci Rep

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White spot syndrome virus (WSSV) is one of the most devastating pathogens in penaeid shrimp and can cause massive damage in shrimp aquaculture industries. Previously, the WSSV structural protein VP15 was identified as an antigenic reagent against WSSV infections. In this study, we truncated this protein into VP15(1–25), VP15(26–57), VP15(58–80), and VP15(1–25,58–80). The purified proteins from the E. coli expression system were assayed as potential protective agents in Kuruma shrimp (Marsupenaeus japonicus) using the prime-and-boost strategy. Among the four truncated constructs, VP15(26–57) provided a significant improvement in the shrimp survival rate after 20 days of viral infection. Subsequently, four peptides (KR11, SR11, SK10, and KK13) from VP15(26–57) were synthesized and applied in an in vivo assay. Our results showed that SR11 could significantly enhance the shrimp survival rate, as determined from the accumulated survival rate. Moreover, a multiligand binding protein with a role in the host immune response and a possible VP15-binding partner, MjgC1qR, from the host M. japonicus were employed to test its binding with the VP15 protein. GST pull-down assays revealed that MjgC1qR binds with VP15, VP15(26–57), and SR11. Taken together, we conclude that SR11 is a determinant antigenic peptide of VP15 conferring antiviral activity against WSSV.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of antigenic domains and peptides from VP15 of white spot syndrome virus and their antiviral effects in Marsupenaeus japonicus

Boonyakida

Satoh

et al. 2021

Sci Rep

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“…The WSSV detection time using this disposable electrode was less than 15 min. For comparison, their DNA copy numbers were measured according to standard RT-PCR [39].…”

Section: Detection Of Wssv Using the Disposable Electrodementioning

confidence: 99%

Electrochemical detection of white spot syndrome virus with a silicone rubber disposable electrode composed of graphene quantum dots and gold nanoparticle-embedded polyaniline nanowires

et al. 2020

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Background With the enormous increment of globalization and global warming, it is expected that the number of newly evolved infectious diseases will continue to increase. To prevent damage due to these infections, the development of a diagnostic method for detecting a virus with high sensitivity in a short time is highly desired. In this study, we have developed a disposable electrode with high-sensitivity and accuracy to evaluate its performances for several target viruses. Results Conductive silicon rubber (CSR) was used to fabricate a disposable sensing matrix composed of nitrogen and sulfur-co-doped graphene quantum dots (N,S-GQDs) and a gold-polyaniline nanocomposite (AuNP-PAni). A specific anti-white spot syndrome virus (WSSV) antibody was conjugated to the surface of this nanocomposite, which was successfully applied for the detection of WSSV over a wide linear range of concentration from 1.45 × 102 to 1.45 × 105 DNA copies/ml, with a detection limit as low as 48.4 DNA copies/ml. Conclusion The engineered sensor electrode can retain the detection activity up to 5 weeks, to confirm its long-term stability, required for disposable sensing applications. This is the first demonstration of the detection of WSSV by a nanofabricated sensing electrode with high sensitivity, selectivity, and stability, providing as a potential diagnostic tool to monitor WSSV in the aquaculture industry.

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“…To date, following the concept of "trained immunity", several kinds of WSSV immunizing agents have been tested, including viral protein subunits, attenuated WSSV, and DNA-/RNA-based agents 24,25 . Most recombinant subunit vaccines investigated have been based on VP19, VP24, VP26, VP28, VP292, and VP466, and among them, VP28 has been widely studied [26][27][28][29][30] . These viral subunits were con rmed to improve the survival rate after challenging shrimp with WSSV.…”

Section: Introductionmentioning

confidence: 99%

Identification of Antigenic Domains and Peptides from VP15 of White Spot Syndrome Virus and their Antiviral Effects in Marsupenaeus Japonicus

Boonyakida

Satoh

et al. 2021

Preprint

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White spot syndrome virus (WSSV) is one of the most devastating pathogens in penaeid shrimp and can cause massive damage in shrimp aquaculture industries. Previously, the WSSV structural protein VP15 was identified as an antigenic reagent against WSSV infections. In this study, we truncated this protein into VP15(1−25), VP15(26−57), VP15(58−80), and VP15(1−25,58−80). The purified proteins from the E. coli expression system were assayed as potential protective agents in Kuruma shrimp (Marsupenaeus japonicus) using the prime-and-boost strategy. Among the four truncated constructs, VP15(26−57) provided a significant improvement in the shrimp survival rate after 20 days of viral infection. Subsequently, four peptides (KR11, SR11, SK10, and KK13) from VP15(26−57) were synthesized and applied in an in vivo assay. Our results showed that SR11 could significantly enhance the shrimp survival rate, as determined from the accumulated survival rate. Moreover, a multiligand binding protein with a role in the host immune response and a possible VP15-binding partner, MjgC1qR, from the host M. japonicus were employed to test its binding with the VP15 protein. GST pull-down assays revealed that MjgC1qR binds with VP15, VP15(26−57), and SR11. Taken together, we conclude that SR11 is a determinant antigenic peptide of VP15 conferring antiviral activity against WSSV.

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Antigenic properties of VP15 from white spot syndrome virus in kuruma shrimp Marsupenaeus japonicus

Cited by 18 publications

References 45 publications

Identification of antigenic domains and peptides from VP15 of white spot syndrome virus and their antiviral effects in Marsupenaeus japonicus

Identification of antigenic domains and peptides from VP15 of white spot syndrome virus and their antiviral effects in Marsupenaeus japonicus

Electrochemical detection of white spot syndrome virus with a silicone rubber disposable electrode composed of graphene quantum dots and gold nanoparticle-embedded polyaniline nanowires

Identification of Antigenic Domains and Peptides from VP15 of White Spot Syndrome Virus and their Antiviral Effects in Marsupenaeus Japonicus

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