Identification of antigenic domains and peptides from VP15 of white spot syndrome virus and their antiviral effects in Marsupenaeus japonicus

Boonyakida, Jirayu; Xu, Jian; Satoh, Jun; Nakanishi, Tetsu; Mekata, Tohru; Kato, Tatsuya; Park, Enoch Y.

doi:10.1038/s41598-021-92002-8

Cited by 9 publications

(3 citation statements)

References 65 publications

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“…Subsequently, ~ 50 μl of the mixture was injected into a fifth-stage instar silkworm larva (Ehimesansyu, Ehime, Japan). The hemolymph containing recombinant BmNPV was collected from the larvae 6–7 days post-injection (dpi) and mixed with 1-phenyl-2-thiourea at 1 mM, with aliquots of hemolymph stored at – 80 °C before use (Boonyakida et al 2021 ; Suhaimi et al 2019 ; Utomo et al 2019 , 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Humoral immune response induced with dengue virus-like particles serotypes 1 and 4 produced in silkworm

Utomo

Pambudi²,

Park

2022

AMB Expr

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Dengue is an arboviral disease, which threatens almost half the global population, and has emerged as the most significant of current global public health challenges. In this study, we prepared dengue virus-like particles (DENV-LPs) consisting of Capsid-premembrane-envelope (CprME) and premembrane-envelope (prME) polypeptides from serotype 1 and 4, which were expressed in the silkworms using Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid. 1CprME, 1prME, 4CprME, and 4prME expressed proteins in hemolymph, and the molecular weight of the purified proteins was 55 kDa, respectively. The purified polypeptides formed spherical Dengue virus-like particles (DENV-LPs) with ~ 30–55 nm in diameter. The immunoelectron microscopy (IEM) images revealed antigens to the surface of a lipid bilayer of DENV-LPs. The heparin-binding assay shows a positive relationship between absorbance and E protein domain III (EDIII) quantity, which is supported by the isothermal titration calorimetry assay. This indicates a moderate binding affinity between heparin and DENV-LP. The high correlation between patient sera and DENV-LP reactivities revealed that these DENV-LPs shared similar epitopes with the natural dengue virus. IgG elicitation studies in mice have demonstrated that DENV-LPs/CPrMEs elicit a stronger immune response than DENV-LP/prMEs, which lends credence to this claim.

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Section: Methodsmentioning

confidence: 99%

Humoral immune response induced with dengue virus-like particles serotypes 1 and 4 produced in silkworm

Utomo

Pambudi²,

Park

2022

AMB Expr

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“…Each recombinant BmNPV bacmid was combined with chitosan (Sigma-Aldrich, Tokyo, Japan) and administered to fth instar silkworm larvae (Ehimesansyu, Ehime, Japan). The hemolymph containing recombinant BmNPV was collected from the larvae 6-7 days after injection (dpi) and mixed with 1-phenyl-2-thiourea at 1 mM, with aliquots of hemolymph stored at -80 °C before use (Boonyakida et al 2021;Suhaimi et al 2019;Utomo et al 2019;Utomo et al 2020).…”

Section: Construction Of Recombinant Bmnpvsmentioning

confidence: 99%

Humoral Immune Response in a Mouse Model Induced With Dengue Virus-like Particles Serotypes 1 and 4 Produced in Silkworm Larvae

Irawan

Pambudi

Park

2021

Preprint

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Dengue is an arboviral disease, which threatens almost half the global population, and has emerged as the most significant of current global public health challenges. In this study, we prepared dengue virus-like particles (DENV-LPs) consisting of Capsid-Premembrane-Envelope (CprM/E) and Premembrane-Envelope (prM/E) polypeptides from serotype 1 and 4, which were expressed in the silkworms using Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid. 1CprME, 1prME, 4CprME, and 4prME expressed proteins in hemolymph and molecular weight of the purified proteins were 55 kDa, respectively. The purified polypeptides formed spherical Dengue virus-like particles (DENV-LPs) with approximately 30–55 nm in diameter. The immunoelectron microscopy (IEM) images revealed antigens to the surface of a lipid bilayer of DENV-LPs. The heparin-binding assay shows a positive relationship between absorbance and the quantity of E protein domain III (EDIII), which was supported by the isothermal titration calorimetry assay, showing a moderate binding affinity between heparin and DENV-LP. The high correlation between patient sera and DENV-LP reactivities revealed that these DENV-LPs shared similar epitopes with the natural dengue virus. IgG elicitation studies in mice have demonstrated that DENV-LP/CPrMEs elicits a stronger immune response than DENV-LP/prMEs, which lends credence to this claim.

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“…White spot disease (WSD) caused by the virus has brought great harm and serious economic losses to shrimp breeding industry in China and all over the world (Millard et al 2021 ). In 1921, WSD was first discovered in China Taiwan (Zhu et al 2019 ), and has spread to other Asian countries (Cui et al 2020 ; Boonyakida et al 2021 ). In 1995, WSD was also found in a shrimp farm in southern Texas (Sanchez-Paz 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Validation of qPCR from a crude extract for the rapid detection of white spot syndrome virus

Tian²,

Yang

et al. 2022

Aquacult Int

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White spot disease (WSD) has posed a serious threat to the China and the global shrimp aquaculture. In order to diagnose white spot syndrome virus (WSSV) early and prevent the spread and outbreak of WSD, it is necessary to establish a highly sensitive WSSV diagnosis method suitable for shrimp farming sites. In this study, a pre-amplification qPCR assay from the crude extract of samples heated lysis was established, which was further compared with the universal qPCR assay to verify the shrimp samples. The limit of detection (LOD) of pre-amplification qPCR assay and universal qPCR assay was 2.80 copies and 20.57 copies per reaction at 95% CI, respectively. It had good WSSV specificity and did not show cross-detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV), hepatopancreatic parvovirus (HPV), Enterocytozoon hepatopenaei (EHP), acute hepatopancreas necrosis disease (AHPND), necrotizing hepatopancreatitis bacteria (NHPB), and decapod iridescent virus 1 (DIV1). A total of 36 shrimp samples were detected as WSSV DNA positive by pre-amplification qPCR with crude extract from samples heated lysis and universal qPCR with DNA extraction. The diagnostic sensitivity and specificity were 97.22% (85.5 ~ 99.9%, 95% CI) and 100% (81.5 ~ 100%, 95% CI), respectively. The agreement Kappa value was 0.959 (0.879 ~ 1, 95% CI), and the analysis results were basically consistent. Eliminating the tedious steps of extracting DNA and using pre-amplified qPCR to detect WSSV in shrimp, it is a good choice for aquaculture farms. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s10499-022-00920-9.

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Identification of antigenic domains and peptides from VP15 of white spot syndrome virus and their antiviral effects in Marsupenaeus japonicus

Cited by 9 publications

References 65 publications

Humoral immune response induced with dengue virus-like particles serotypes 1 and 4 produced in silkworm

Humoral immune response induced with dengue virus-like particles serotypes 1 and 4 produced in silkworm

Humoral Immune Response in a Mouse Model Induced With Dengue Virus-like Particles Serotypes 1 and 4 Produced in Silkworm Larvae

Validation of qPCR from a crude extract for the rapid detection of white spot syndrome virus

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