Antigenic Properties of Pleuropneumonia-Like Organisms From Tissue Cell Cultures and the Human Genital Area

Abstract

properties of pleuropneumonia-like organisms from tissue cell cultures and the human genital area. J. Bacteriol. 82:542-547. 1961.-Antigens were prepared from several tissue culture and human genital strains of pleuropneumonia-like organisms (PPLO) by a method utilizing continuous agitation of the incubating cultures. Antisera were produced in rabbits by intravenous injection of suspensions of these organisms standardized turbidimetrically. The antigenic properties of the selected strains were compared by agglutination techniques supplemented by a test based upon the inhibition of growth of PPLO by specific antisera. The majority of tissue culture strains of PPLO studied, including contaminants from several HeLa cell lines, appeared to be antigenically similar to the human type 1 strains. However, one strain (Sp-1) from a HeLa cell line was found to be related to the human type 2 PPLO.

Physiological and Serological Comparisons Among Strains ofMycoplasma granularumandMycoplasma laidlawii

“…Washed mycoplasma antigens diluted to a 50%l ight transmittance at 450 m,u in a Coleman Junior spectrophotometer were used for preparation of hyperimmune antisera. The antigens were administered to rabbits over a 2-week period (2).…”

GROWTH INHIBITORY PROPERTIES OF MYCOPLASMA ANTIBODY

“…hominis tyl)e II strain, designated CH. The origin of these strains and the methods used to prepare antigen and rabbit antisera have been described previously by Bailey et al (1961). Commercial pooled rabbit serum was used for the normal control in the agglutination and inhibition tests.…”

Serologic and immunologic studies on Mycoplasma hyopneumoniae pneumonia of swine

“…Agglutination test Helsop (1922) to be a satisfactory method of serodiagnosis and was believed to be more sensitive than CF. Newing and Field (1953) The agglutination test has also been used to study the antigens of mycoplasma from human and tissue culture sources (Bailey et_ al. 1961;Tally 1963).…”