Antigenic fractions from Taenia crassiceps metacestodes obtained by hydrophobicity for the immunodiagnosis of active and inactive forms of neurocysticercosis in human cerebrospinal fluid samples

Silva, Gabriela B. da; Nunes, D. S.; Sousa, José Eduardo Neto de; Gonçalves-Pires, Maria do Rosário F.; Levenhagen, Marcelo Arantes; Costa-Cruz, Júlia Maria

doi:10.1016/j.parint.2016.12.009

Cited by 7 publications

(3 citation statements)

References 24 publications

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“…Considering the presence of common antigenic determinants shared with T. solium metacestodes, along with the minimal cost of antigen production in laboratory, our results indicate the hydrophobic fraction of T. crassiceps as a potential diagnostic tool for NCC diagnosis. Antigenic fractions of T. crassiceps have been successfully tested in CSF samples of NCC-positive patients, showing high values of Se and Sp in immunoassays using the hydrophobic antigen (Silva et al ., 2017).…”

Section: Discussionmentioning

confidence: 99%

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IgY antibody and human neurocysticercosis: a novel approach on immunodiagnosis usingTaenia crassicepshydrophobic antigens

Carrara¹,

Silva²,

Faria³

et al. 2019

Parasitology

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Human neurocysticercosis (NCC) is a worldwide neglected disease caused by Taenia solium metacestode and responsible for various complications and neurological disorders. This study aimed to evaluate the use of specific immunoglobulin Y (IgY) produced by laying hens immunized with a hydrophobic fraction of Taenia crassiceps metacestodes (hFTc) in NCC diagnosis. Egg yolk IgY antibodies were fractionated, purified and characterized. Enzyme-linked immunosorbent assay (ELISA) was carried out to evaluate the production kinetics and avidity maturation of anti-hFTc IgY antibodies throughout the IgY obtention process. Antigen recognition tests were carried out by Western blotting and immunofluorescence antibody test using purified and specific anti-hFTc IgY antibodies for detection of parasitic antigens of T. crassiceps and T. solium metacestodes. Sandwich ELISA was performed to detect circulating immune complexes formed by IgG and parasitic antigens in human sera. The results showed high diagnostic values (93.2% sensitivity and 94.3% specificity) for immune complexes detection in human sera with confirmed NCC. In conclusion, specific IgY antibodies produced from immunized hens with hFTc antigens were efficient to detect T. solium immune complexes in human sera, being an innovative and potential tool for NCC immunodiagnosis.

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Section: Discussionmentioning

confidence: 99%

“…The hydrophobic antigen (hF Tc ) was obtained from the total saline extract of T. crassiceps metacestodes (Te Tc ), using Triton X-114 (TX-114) fractionation method as described by Silva et al . (2017). The fractionation process started with a protein mass of 14 mg mL −1 from Te Tc .…”

Section: Methodsmentioning

confidence: 99%

IgY antibody and human neurocysticercosis: a novel approach on immunodiagnosis usingTaenia crassicepshydrophobic antigens

Carrara¹,

Silva²,

Faria³

et al. 2019

Parasitology

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“…Also, the murine cysticercosis model is easily maintained in the laboratory, allowing the measurement of parasite loads without biohazard risks (Capelli-Peixoto et al , 2011). The experimental model T. crassiceps -BALB/c mice was selected because this is a relatively simple laboratory model of cysticercosis (Sciutto et al , 1990; Silva et al , 2017; Carrara et al , 2020). Additionally, T. crassiceps does not readily parasitize humans (Sciutto et al , 2008; Willms and Zurabian, 2010).…”

Section: Introductionmentioning

confidence: 99%

Mimotope-based antigens as potential vaccine candidates in experimental murine cysticercosis

Manhani¹,

Tilelli²,

Ribeiro³

et al. 2020

Parasitology

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Human cysticercosis is a public health problem caused by Taenia solium metacestodes; thus, eradication of T. solium transmission by vaccination is an urgent requirement. The Cc48 mimotope from T. solium cysticerci was tested expressed in phage particles (mCc48) and chemically synthesized (sCc48) as a vaccine candidate in experimental murine cysticercosis. For this, BALB/c mice were immunized with mCc48 (G1; n = 40), sCc48 (G2; n = 40) and phosphate-buffered saline (PBS) (G3; n = 40, positive control) and challenged with Taenia crassiceps metacestodes. Another PBS group without parasite challenge was used as a negative control (G4; n = 40). Mice were sacrificed 15, 30, 45 and 60 days post-infection for cysticerci and serum collection. Immunization efficacy was determined by cysticerci counting. Serum samples were tested by ELISA to verify antibody (IgM, IgG, IgA and IgE) and cytokine (IFNγ and IL-4) levels. The sCc48 achieved the highest rates of protection and efficacy (90 and 98%, respectively). The group immunized with mCc48 presented the highest reactivity for IgM, IgG and IgE. All groups presented IL-4, but IFNγ was quite variable among groups. The protection induced by sCc48 synthetic peptide supports further studies of this mimotope as a potential vaccine candidate against cysticercosis.

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Taeniasis and Cysticercosis

Ferrer

Perteguer

2022

Helminth Infections and Their Impact on Global Public Health

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Antigenic fractions from Taenia crassiceps metacestodes obtained by hydrophobicity for the immunodiagnosis of active and inactive forms of neurocysticercosis in human cerebrospinal fluid samples

Cited by 7 publications

References 24 publications

IgY antibody and human neurocysticercosis: a novel approach on immunodiagnosis usingTaenia crassicepshydrophobic antigens

IgY antibody and human neurocysticercosis: a novel approach on immunodiagnosis usingTaenia crassicepshydrophobic antigens

Mimotope-based antigens as potential vaccine candidates in experimental murine cysticercosis

Taeniasis and Cysticercosis

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