Antigenic Comparison of Rauscher Murine Leukemia Virus Cultivated in Human Embryo and Mouse Cells

Propagation of Rauscher Leukemia Virus to Human Lymphoblastoid Cells by in Vivo Diffusion Chamber Cultivation

“…c ' 5 -3% from the plasma mem- The leukemogenicity of C type particles continuously produced by human lymphoblastoid cells was tested by inoculation into newborn BALB/c mice. It cannot be inferred which of these two mechanisms is right for the present experiment, because both are known to occur; the leukemogenicity of RLV can be either lost (4, 6,20) or reduced ( 2 1 ) when it is propagated in human embryo cell cultures. Mice that did not develop Rauscher leukemia a t 2 months after inoculation of human cell-passaged C type virus were challenged with ip inoculation of 0.1 ml of 10fold dilution of 10% Rauscher leukemic spleen extract.…”

New method for the removal of extraneous proteins from purified oncornaviruses

“…Virus proteins were also examined by electrophoretic analysis on 10% polyacrylamide gels 254 TRAUL ET AL. containing 0.1% sodium dodecyl sulfate (SDS; 14). Equal amounts (100 ,ug) of protein from either salttreated or control preparations were disrupted and electrophoresed.…”

“…Serologic testing. The microimmunodiffusion and complement fixation methods used to compare test and control preparations have been described previously (14). All virus preparations were disrupted with ether prior to serologic testing.…”

“…Antisera to RD virus, from Hartly guinea pigs, and antisera to RLV, from New Zealand white rabbits, were prepared by weekly injections of ether-treated or NaClether-treated virus (footpad with complete Freund adjuvant then subcutaneously for 5 weeks). Antiserum to RLV was also obtained from Osborne-Mendel rats bearing transplantable RLV-induced tumors (14).…”