Comparative Biology of Murine and Avian Tumor Viruses

Bentvelzen, P.

doi:10.1016/b978-0-12-429760-9.50018-2

Viruses, Evolution and Cancer Basic Considerations 1974

DOI: 10.1016/b978-0-12-429760-9.50018-2

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Comparative Biology of Murine and Avian Tumor Viruses

P. Bentvelzen¹

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1975

1996

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Cited by 3 publications

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“…A number of assays for such viruses are in common use, but they have disadvantages for quantitative applications. Infectivity assays, although necessary to measure the biological activity of viruses, are time-consuming (1 week to 12 months) and usually cannot be analyzed in terms of the number of virus particles in the sample (1,8). Assays for incorporation of radioactive uridine into particles that band at a density of 1.15 to 1.18 in sucrose are useful as a relative measure of virus production (11), but they require that the virus be grown in tissue culture (a condition that frequently cannot be met) and presume that uridine incorporation into viral particles is linear over the iabeling period.…”

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Quantitation of RNA tumor viruses by spectroscopy of density gradient gels

Liebes¹,

Retzel²,

Maher³

et al. 1975

J Virol

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We have developed a system for virus particle quantitation based on the measurement of the optical absorbance of stained viruses which first have been banded at their buoyant density in an equilibrium 24 to 53% (wt/wt) sucrose density gradient, then fixed in position in the gradient by photopolymerizing an acrylamide-riboflavin mixture in the sucrose, and finally stained and destained. Using plasma from mice infected with leukemia virus (Rauscher) or chickens infected with avian myeloblastosis virus (BAI strain) or suitable controls, we have shown that this technique specifically detects RNA tumor viruses. By using virus stock solutions for which the absolute concentrations were determined by laser beat frequency spectroscopy, we have calibrated the absorbance of the viral bands in terms of virus particle concentration. Using 0.8-ml gradients gels (4 by 45 mm) we can detect as low as 2 x 107 viral particles with Coomassie blue staining and 6 x 106 viral particles with a more sensitive staining procedure using amido black.

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confidence: 99%

Quantitation of RNA tumor viruses by spectroscopy of density gradient gels

Liebes¹,

Retzel²,

Maher³

et al. 1975

J Virol

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Evidence for an oncovirus in swimbladder fibrosarcoma of Atlantic salmon Salmo salar L.

Duncan

1978

Journal of Fish Diseases

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several cases of swimbladder neoplasia were found in pen-reared Atlantic salmon i^almo salar L. on a commercial marine fish farm in Scotland, in the summer and autumn of 1976. A histological examination showed the condition to be a fibrosarcoma (McKnight, 1978). The multiple incidence of tumours prompted an electron microscopic survey, in an attempt to discover if the condition had been caused by an infectious agent; this communication reports preliminary evidence in support of such an aetiology.Suspect fish were brought back alive to the laboratory, where they were killed and tbeir swimbladders removed. Pieces of tissue were fixed with 2-5% phosphatebuffered glutaraldehyde, postfixed with 1% buffered osmic acid, then alcoholdehydrated and embedded in Epon 812 epoxy resin. Sections were cut on an LKB Ultrotome III, then stained with a saturated solution of uranyl acetate in methanol, followed by alkaline lead citrate (Reynolds 1963), and examined in a Siemens Elmiskop 1 electron microscope.

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Mouse mammary tumor virus and mammary tumorigenesis in wild mice

Imai

1996

Pathology International

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The current knowledge of the distribution of the mouse mammary tumor virus (MMTV) proviral genomes and the mechanism of mammary tumorigenesis by MMTV in mice, with the main emphasis on Asian feral mice, is reviewed. The relevant earlier discoveries on the mode of MMTV transmission are summarized to provide an outline of the biology of MMTV. Finally, the viral etiology of human breast cancer will be discussed.

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Comparative Biology of Murine and Avian Tumor Viruses

Cited by 3 publications

References 353 publications

Quantitation of RNA tumor viruses by spectroscopy of density gradient gels

Quantitation of RNA tumor viruses by spectroscopy of density gradient gels

Evidence for an oncovirus in swimbladder fibrosarcoma of Atlantic salmon Salmo salar L.

Mouse mammary tumor virus and mammary tumorigenesis in wild mice

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