Antigenic characterization of swine rotaviruses in Argentina

Mattion, Nora; Bellinzoni, R. C.; Blackhall, Jorge; Torre, J.L. La; Scodeller, Eduardo A.

doi:10.1128/jcm.27.4.795-798.1989

Cited by 32 publications

(28 citation statements)

References 27 publications

(31 reference statements)

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“…The results from PAGE analysis showing that none of the 25 PAGE positive samples had the vaccine virus electropherotype agreed with a previous report which suggested that the vaccine was probably not the cause of rotaviral diarrhea, and it was frequently inefficacious in preventing the infections in the herds [14]. Short electropherotype rotaviruses similar to the rotaviruses previously reported in humans, rabbits, pigs and calves [12][13][14][15][16][17][18][19] were found in all day 1 PAGE positive and most of day 7 PAGE positive samples obtained from calves while long electropherotype rotaviruses were found at days 7 and 14. Regardless of their electropherotypes, the presence of these short and long electropherotype rotaviruses might be due to their antigenic difference from common rotaviruses contaminating the farm environment; thus, they were able to evade passive immunity.…”

Section: Pagesupporting

confidence: 89%

“…Super-short electropherotype rotavirus isolated from a calf, however, was demonstrated to be the result of the altered mobility of genome segment 1 I which migrated between genome segments 9 and 10 [12]. Short electropherotypes have been recognized in mice, rabbits, calves, humans, pigs and birds [5,[12][13][14][15][16][17][18][19][20][21]. Non-group A rotaviruses lack the 7-8-9 triplet pattern at area III found in group A viruses [3,8].…”

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confidence: 99%

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Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California

Chinsangaram

Schore

Guterbock

et al. 1995

Comparative Immunology, Microbiology and Infectious Diseases

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136 fecal samples, collected from 47 dairy calves on a calf ranch and in a dairy herd in California, were tested for the presence of group A and group B rotaviruses by reverse transcription-polymerase chain reaction (RT-PCR). Samples were collected from each calf at days 1, 7 and 14. Within the 14 day period, 44 calves (94%) were positive for group A rotavirus and an unexpectedly high number of calves (38 calves, 81%) were positive for group B rotavirus. When these samples were examined by polyacrylamide gel electrophoresis (PAGE), rotavirus was found in 21 calves and all of them had group A electropherotype. Among 25 PAGE positive samples from 21 calves, 17 (68%) were of short electropherotype, 4 (28%) were of long electropherotype and 4 (28%) contained both short and long electropherotype rotaviruses. Group B and short and long electropherotype group A rotaviruses were found in both normal and diarrheic calves.

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Section: Pagesupporting

confidence: 89%

mentioning

confidence: 99%

Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California

Chinsangaram

Schore

Guterbock

et al. 1995

Comparative Immunology, Microbiology and Infectious Diseases

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“…The role of group C rotavirus as animal and human pathogen was established before [Mattion et al, 1989;Gouvea et al, 1991;Jiang et al, 1995Jiang et al, , 1996Kuzuya et al, 1998]. In the current study, assessment of group C rotavirus as causative agent of the diarrheal disease was not fully accomplished because paired sera from infected children were not avail- Fig.…”

Section: Discussionmentioning

confidence: 93%

“…In a previous report [Sorrentino et al, 1986] an atypical electropherotype, compatible with the pattern (4-3-2-2) of group C rotaviruses was described in a single stool sample taken from a patient in the northeastern Chaco province. Nevertheless, group C rotavirus has been isolated and characterized in Argentine pig herds [Mattion et al, 1989] in geographical regions closer to the location where the human isolates described in this study were identified.…”

Section: Introductionmentioning

confidence: 90%

Characterization of human group C rotavirus in Argentina

Castello

Argüelles

Villegas³

et al. 2000

J. Med. Virol.

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A survey was conducted for identification of human group C rotaviruses in stool specimens taken from children suffering diarrhea in suburban Buenos Aires regions. Among 90 true negative group A samples as defined by ELISA, RT-PCR and PAGE, five were positive by group C specific RT-PCR (VP7 and VP6 genes) and three of these samples exhibited the characteristic 4-3-2-2 dsRNA pattern of group C rotavirus. These results were further confirmed by electron microscopy and by ELISA for detection of group C VP6 specific antigens. Sequence analysis of the VP7 gene from one of these isolates revealed a 97.3-98.6% nucleotide identity and up to 99.1% protein homology with human group C rotavirus strains found scattered throughout the last ten years in other countries. Conversely, similar analysis performed with porcine strains showed a much lower homology degree both at the nucleotide (75.5% nucleotide identity) and amino acid level (85.5% protein homology). Detection of group C rotavirus in children with acute diarrhea in Argentina extends the identification range of this agent in the region and is consistent with previous reported data that demonstrate a global distribution of this virus.

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“…These viruses, the prototype Gottfried (G4, P5) and OSU (G5, P7) strains, were initially reported in 1984 and 1977, respectively. Rotaviruses with similar G-serotype specificities have been detected in swine in several other countries (10,14). Recently, two other G serotypes of rotavirus were isolated from swine and characterized.…”

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confidence: 94%

Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine VP4 and human and porcine VP7 genes

Rosen¹,

Parwani²,

López³

et al. 1994

J Clin Microbiol

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Of 216 fecal and intestinal samples collected from nursing or weaned diarrheic pigs in the United States and Canada, 57 were identified as group A rotavirus positive by RNA electrophoresis and silver staining. Fifty-seven and 52 rotavirus-positive samples were analyzed by hybridization with Gottfried and OSU PCR-derived gene 9 and 4 probes, respectively. Only 17 samples were identified with either homologous VP4 (P)or VP7 (G)-coding genes or both. One rotavirus identified as G4 and P7 was similar to the previously characterized interserotype rotavirus, SB-1A. Additional hybridization analyses were performed with PCRderived probes prepared from gene 9 cDNA of the human rotaviruses Wa (Gi), DS-1 (G2), and P (G3) and of the porcine rotavirus YM (Gll). Eleven of 52 samples collected and analyzed from swine in Ohio, California, and Nebraska were identified as Gll. No samples with Gi-, G2-, or G3-type specificities were detected among the 25 of 57 rotavirus-positive samples analyzed with human rotavirus-derived probes. Further investigations with a PCR-derived gene 4 probe prepared from porcine rotavirus YM revealed hybridization specificities similar to those of the OSU gene 4 probe.

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Antigenic characterization of swine rotaviruses in Argentina

Cited by 32 publications

References 27 publications

Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California

Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California

Characterization of human group C rotavirus in Argentina

Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine VP4 and human and porcine VP7 genes

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