Antigenic and Immunogenic Investigation of B-Cell Epitopes in the Nucleocapsid Protein of Peste des Petits Ruminants Virus

Choi, Kang-Seuk; Nah, Jin‐Ju; Ko, Young-Joon; Kang, Shien-Young; Yoon, Kyoung‐Jin; Jo, Nam-In

doi:10.1128/cdli.12.1.114-121.2005

Cited by 30 publications

(23 citation statements)

References 42 publications

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“…Blocking and competitive ELISA revealed that epitopes on domain A-II and C-II were immunodominant. The A-II domain was shown to cross react with RPV antibodies [32].…”

Section: Immunitymentioning

confidence: 99%

Diagnosis and control of peste des petits ruminants: a comprehensive review

Balamurugan¹,

Hemadri²,

Gajendragad³

et al. 2013

VirusDis.

131

118

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Peste des petits ruminants (PPR) is an acute, highly contagious, world organization for animal health (OIE) notifiable and economically important transboundary viral disease of sheep and goats associated with high morbidity and mortality and caused by PPR virus. PPR is considered as one of the main constraints in augmenting the productivity of small ruminants in developing countries and particularly severely affects poor farmer's economy. The disease is clinically manifested by pyrexia, oculo-nasal discharges, necrotizing and erosive stomatitis, gastroenteritis, diarrhoea and bronchopneumonia. The disease can be diagnosed from its clinical signs, pathological lesions, and specific detection of virus antigen/antibodies/genome in the clinical samples by various serological tests and molecular assays. PPR is the one of the priority animal diseases whose control is considered important for poverty alleviation in enzootic countries. Availability of effective and safe live attenuated cell culture PPR vaccines and diagnostics have boosted the recently launched centrally sponsored control programme in India and also in other countries. This review article primarily focus on the current scenario of PPR diagnosis and its control programme with advancement of research areas that have taken place in the recent years with future perspectives.

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“…Blocking and competitive ELISA revealed that epitopes on domain A-II and C-II were immunodominant. The A-II domain was shown to cross react with RPV antibodies [32].…”

Section: Immunitymentioning

confidence: 99%

Diagnosis and control of peste des petits ruminants: a comprehensive review

Balamurugan¹,

Hemadri²,

Gajendragad³

et al. 2013

VirusDis.

131

118

View full text Add to dashboard Cite

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“…MAb P-3H12 was used as a competing antibody. MAb P-3H12 recognizes an immunodominant epitope within the amino-terminal half of the N protein, as described previously (5). A total of approximately 6 mg of the MAb purified from mouse ascitic fluid was coupled with 8 mg of activated peroxidase.…”

Section: Resultsmentioning

confidence: 99%

Rapid Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Peste des Petits Ruminants Virus

Choi

Nah

et al. 2005

Clin Vaccine Immunol

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Peste des petits ruminants (PPR) is a contagious viral disease of small ruminants that is of economic importance in Africa, the Middle East, and Asia. We developed a rapid competitive enzyme-linked immunosorbent assay (rapid c-ELISA) for the diagnosis and surveillance of PPR. This assay detects PPR virus (PPRV) antibodies in serum samples by quantifying the amount of monoclonal antibody (MAb) P-3H12 after 30 min of incubation of a serum-MAb conjugate mixture on plates coated with a PPRV recombinant nucleocapsid protein (rPPRV-N). We tested 249 PPRV-positive serum samples and 733 PPRV-negative serum samples from field ruminants. The threshold of percent inhibition (PI) was determined to be <50 on the basis of the mean PI plus 3 standard deviations for sera from PPRV-negative ruminants. The relative specificity and sensitivity of the rapid c-ELISA were 98.5% (722 of 733 serum samples) and 93.4% (234 of 249 serum samples), respectively. The rapid c-ELISA sensitively detected PPRV antibodies in hyperimmune sera (virus neutralization test [VNT] titer, >512), even at dilutions >512 in normal goat serum, and as early as 6 to 13 days postinfection from 12 goats, each of which was infected with one of the four PPRV lineages. Hyperimmune sera from animals experimentally vaccinated with rinderpest virus gave positive results by the rapid c-ELISA when the rinderpest virus VNT titers were >512, although the rapid c-ELISA titers were very low (2 to 16). However, the rapid c-ELISA was negative when the rinderpest virus VNT titer was <128. The rapid c-ELISA developed in the present work provides a short turnaround time and could be a useful tool for the diagnosis of PPR and screening for PPRV in the field.Peste des petits ruminants (PPR) is an acute and highly contagious viral disease of small ruminants, such as sheep and goats, with high rates of morbidity and sometimes high rates of mortality. Economically, it has been the most important disease of these species in sub-Saharan Africa, the Middle East, and southwest Asia since it was first described in West Africa in 1942 (20). It also results in subclinical infection in large ruminants, which act as carriers of infection to small ruminants (1, 14). The PPR virus (PPRV) belongs to the genus Morbillivirus in the family Paramyxoviridae and is closely related to rinderpest virus (RPV) (2, 16). At least four distinct genetic lineages (lineages I, II, III, and IV) of PPRV with different geographical distributions circulate among small ruminants in regions of endemicity (6,20).A vigorous program for PPR eradication has been carried out in these regions, including vaccination, seromonitoring, serosurveillance, and the destruction of infected animals and those that have been in contact with them. Rapid detection of infected animals is very important for PPR controls to be effective. Severe cases in which animals show clinical signs in the field can easily be detected through clinical surveillance and the detection of antigen in clinical samples, while the diagnosis of PPRV infection ...

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“…Region I includes amino acids 1-120, region II includes 121-145, region III comprises amino acids 146-398, and region IV finishes with amino acids 421-525. Recently, it has been demonstrated that regions I and II are comparatively more immunogenic than regions III and IV (Choi et al, 2005b). Another study demonstrated that the amino acids from 452-472 are the most immunogenic part within the N protein.…”

Section: Host Response To Pprv and Potential For Serodiagnosismentioning

confidence: 99%

“…ELISAs using monoclonal antibodies have been used for serological diagnosis and antigen detection for diagnostic and screening purposes. For PPR antibody detection, competitive ELISA is a better choice as it is sensitive, specific, reliable, and has a high diagnostic specificity (99.8%) and sensitivity (90.5%) (Sreenivasa et al, 2006;Choi et al, 2005b;Brindha et al, 2001). Immunocapture ELISA is a rapid, sensitive and virus specific test for PPRV antigen detection, and it can differentiate between RP and PPR viruses.…”

Section: Laboratory Diagnosis Of Pprmentioning

confidence: 99%