Antigen-specific detection of soluble immune complexes by a solid phase specific antibody system

Pernice, W.; Sedlacek, H.H.

doi:10.1016/0022-1759(79)90325-9

Cited by 23 publications

(3 citation statements)

References 17 publications

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“…Moreover, in all the patients who retained their ICs, as noted by Pernice and Sedlacek (1979), a good correlation was found between the level of HBsAg-containing immune complexes and the clinical state. ICs could be detected simultaneously with HBsAg and either decreased or disappeared before the appearance of free antiHBs.…”

Section: Discussionmentioning

confidence: 83%

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A study of the evolution of specific and non-specific immune complexes in acute hepatitis B and chronic hepatitis

Leyva

Bernal²,

Piédrola³

et al. 1988

Journal of Medical Microbiology

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Section: Discussionmentioning

confidence: 83%

“…It is generally assumed that ICs in slight antigen excess may be responsible for the development of IC disease (Pernice and Sedlacek, 1979).…”

Section: Discussionmentioning

confidence: 99%

A study of the evolution of specific and non-specific immune complexes in acute hepatitis B and chronic hepatitis

Leyva

Bernal²,

Piédrola³

et al. 1988

Journal of Medical Microbiology

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“…Untreated bovine sera from the F. hepatica-infected calves were plated at 1/40 dilutions (optimum) followed by HRP-labeled anti-bovine antibody as described. This assay was designed to detect the soluble host antibody molecules linked to antigen immobilized by the solid-phase antibody according to the principles outlined by Pernice and Sedlacek (1979).…”

Section: Detection Of Antigen In Immune Complexesmentioning

confidence: 99%

Detection of Circulating Immune Complexes by the Enzyme-Linked Immunosorbent Assay in Sera from Cattle Infected with Fasciola hepatica

Hillyer¹

1989

The Journal of Parasitology

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Polyethylene glycol-precipitated immune complexes (PIC) from the sera of 5 calves with Fasciola hepatica worm burdens ranging between 27 and 70 flukes were examined for parasite antigen content at 2, 4, 6, 8, 10, and 16 wk postinfection (PI) by the enzyme-linked immunosorbent assay (ELISA). Three assays were devised using an affinity-processed rabbit antibody to worm excretory/secretory (FhES) antigens. The PIC plate assay detected parasite antigen by adherence of anti-FhES antibody to PIC incubated overnight on ELISA plates, and tests were visualized using anti-rabbit peroxidase-linked antibody. The serum complex and PIC capture assay utilized the anti-FhES immunoglobulin as an antigen capture antibody linked to the solid phase. The attached complexes were then detected by the adhering bovine antibody, either soluble complexes in serum or as PIC. All assays showed circulating immune complex (CIC) values elevated at 6-8 wk PI, which generally coincided with increased host circulating antibody to FhES antigens. The greatest detection rate for all of the immune complex (IC) detection assays occurred with the PIC capture assay. It detected antigen in almost 90% of sera tested at 6 and 8 wk PI. Both the serum complex and PIC capture assay detected greatest amounts of CIC in those animals with the largest worm burdens, whereas the PIC plate assay showed no such trend. This study shows that F. hepatica antigen detection in CIC can be used to aid immunologic diagnosis of fascioliasis.

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Tumor therapy of neoplastic diseases with tumor cells and neuraminidase

Sedlacek

Hagmayer

Seiler

1986

Cancer Immunol Immunother

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The therapeutic effect of i.d. injection of tumor cells mixed with Vibrio cholerae neuraminidase (VCN) on tumor progression in dogs with spontaneous mammary tumors was investigated. The i.d. injections were performed in a chessboard-like manner: different numbers (10(5), 10(6), 10(7), and 10(8) of mitomycin-treated autologous tumor cells (M-TC) were each mixed with different amounts (10, 50, and 100 mU) of VCN. These different mixtures were injected i.d. at different sites in dogs on the day of resection of a part of multiple tumors.

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Antigen-specific detection of soluble immune complexes by a solid phase specific antibody system

Cited by 23 publications

References 17 publications

A study of the evolution of specific and non-specific immune complexes in acute hepatitis B and chronic hepatitis

A study of the evolution of specific and non-specific immune complexes in acute hepatitis B and chronic hepatitis

Detection of Circulating Immune Complexes by the Enzyme-Linked Immunosorbent Assay in Sera from Cattle Infected with Fasciola hepatica

Tumor therapy of neoplastic diseases with tumor cells and neuraminidase

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