Antigen detection assay with parasite specific monoclonal antibodies for diagnosis of lymphatic filariasis

Pandey, Vaibhav; Madhumathi, Jayaprakasam; Karande, Anjali A.; Kaliraj, Perumal

doi:10.1016/j.cca.2011.06.029

Cited by 16 publications

(24 citation statements)

References 14 publications

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“…It also had higher affinity and avidity to recombinant antigen [12]. The polyclonals were prepared by immunizing laboratory-bred rabbits with purified r Wb SXP-1 protein [23].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Rapid Blood Sample Collection in the Detection of Circulating Filarial Antigens for Epidemiological Survey by rWbSXP-1 Capture Assay

et al. 2014

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BackgroundLymphatic filariasis is a neglected tropical disease leading to profound disfiguring causing socio economic burden in the tropics. Current diagnosis strategies available during field surveys and epidemics are based on traditional microscopic detections and a few antigen/antibody assays. We have compared different sampling methodologies and standardized the highly sensitive and reliable rWbSXP-1 antigen detection assay to our new sampling methodology.MethodologySamples collected as serum, whole blood, whole blood on filter paper and whole blood on microscopic slides from patients belonging to various clinical groups of filariasis [endemic normal(EN), chronic pathology(CP), microfilaraemic(MF) and non-endemic normal(NEN)] were collected and standardized the rWbSXP-1 antigen detection assay using monoclonal antibody raised against rWbSXP-1 protein. The whole blood collected on microscopic slide based sampling method was employed in the field and the presence of circulating filarial antigen (CFA) was assessed using the rWbSXP-1 assay.Principal FindingsThe sampling methods were compared and no significant difference was observed for the detection of CFA (MF, P = 0.304, EN, P = 0.675, CP, P = 0.5698, NEN, P = 0.4494). Further the optimized sampling method was utilized to collect the 1106 samples from Polur, Tiruvannamalai. The rWbSXP-1 assay gave 98 antigen positive results whereas the microscopic method gave only 17.ConclusionsFour sampling methodologies were analyzed and the new sampling methodology of whole blood collected on microscopic slide was found to be convenient for the detection of CFA using rWbSXP-1 antigen detection assay. The 1106 samples from Polur were collected using the new method. The rWbSXP-1 antigen assay perceived a 7.32% increased result which was read as false negatives on the conventional microscopic staining method. This new sampling methodology coupled with the rWbSXP-1 antigen assay can be used in epidemiological surveys for lymphatic filariasis and the same sampling methodology can be expanded to other antigen based high affinity assays.

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“…It also had higher affinity and avidity to recombinant antigen [12]. The polyclonals were prepared by immunizing laboratory-bred rabbits with purified r Wb SXP-1 protein [23].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Rapid Blood Sample Collection in the Detection of Circulating Filarial Antigens for Epidemiological Survey by rWbSXP-1 Capture Assay

et al. 2014

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“…In contrast to the therapeutic field, the field of parasite diagnostics has seen the development of a range of monoclonal antibodies against many of the neglected tropical diseases caused by parasites, including schistosomiasis [72], echinococcosis [73], taeniasis [74], leishmaniasis [75], lymphatic filariasis [76], and onchocerciasis [77]. These and other diagnostic developments may indeed help improve patient stratification, clinical diagnosis, and overall patient care within parasitology.…”

Section: Parasitesmentioning

confidence: 99%

How can monoclonal antibodies be harnessed against neglected tropical diseases and other infectious diseases?

Laustsen

2019

Expert Opinion on Drug Discovery

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Highlights 1. Therapies based on monoclonal antibodies against bacterial and viral agents have already entered the clinic, while no monoclonal antibodies against parasites, animal envenoming, or mushroom poisonings have been tested in the clinical setting 2. Neglected tropical diseases represent a golden opportunity for antibody developers, as there are many scientifically low-hanging fruits in this field 3. An increasing number of therapeutic monoclonal antibody discoveries has been reported for neglected tropical diseases and other infectious diseases 4. Monoclonal antibodies may be particularly useful for developing therapies against intoxications caused by both animals and bacteria 5. Low cost of manufacture and selective cross-reactivity are key challenges for developing monoclonal antibodies against neglected tropical diseases and other infectious diseases 6. Treatment against snakebite envenoming and bacterial and viral infections will likely require the use of oligoclonal antibodies for multi-target neutralization Abstract Introduction: Monoclonal antibody-based therapies now represent the single-largest class of molecules undergoing clinical investigation. Although a handful of different monoclonal antibodies have been clinically approved for bacterial and viral indications, as well as rabies, therapies based on monoclonal antibodies are yet to fully enter the fields of neglected tropical diseases and other infectious diseases. Areas covered: This review presents current state-of-the-art in the development and use of monoclonal antibodies against neglected tropical diseases and other infectious diseases, including viral, bacterial, and parasitic infections, as well as envenomings by animal bites and stings. Additionally, a short section on mushroom poisonings is included. Key challenges for developing antibody-based therapeutics is discussed for each of these fields. Expert opinion: Neglected tropical diseases and other infectious diseases represent a golden opportunity for academics and technology developers for advancing our scientific capabilities within the understanding and design of antibody cross-reactivity, use of oligoclonal antibody mixtures for multi-target neutralization, novel immunization methodologies, targeting of evasive pathogens, and development of fundamentally novel therapeutic mechanisms of action. Furthermore, a huge humanitarian and societal impact is to gain by exploiting antibody technologies for the development of biotherapies against diseases, for which current treatment options are suboptimal or non-existent.

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“…It is one of the most debilitating and disfiguring scourges among all diseases. The infection is caused by three helminthic worms Wuchereria bancrofti, Brugia malayi and Brugia timori inhabiting the lymphatic system . Lymphatic filariasis has lifelong implications, such as lymphoedema of the limbs, genital disease, and painful recurrent attacks.…”

Section: Tropical Diseasesmentioning

confidence: 99%

Electrochemical Biosensing for the Diagnosis of Viral Infections and Tropical Diseases

2017

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Rapid and reliable diagnosis of viral infections and tropical diseases and timely initiation of appropriate treatment are essential for their successful clinical management. This Review summarizes some basic concepts regarding the main viral infection and tropical diseases to which society is facing today and the conventional methods available for their detection. The tremendous potential offered by electrochemical affinity biosensors to address this important challenge, meeting the required demands for viral infections and tropical diseases diagnosis, is clearly stated by discussing challenges, opportunities, implementation, and application of selected examples focused on the determination of specific biomarkers of different molecular (genetic, regulatory, and functional) levels. The highlighted approaches demonstrate the use of a plethora of specific receptors and assay formats and a great diversity of attractive electrochemical approaches in this rapidly advancing and highly interesting field.

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Antigen detection assay with parasite specific monoclonal antibodies for diagnosis of lymphatic filariasis

Cited by 16 publications

References 14 publications

Evaluation of Rapid Blood Sample Collection in the Detection of Circulating Filarial Antigens for Epidemiological Survey by rWbSXP-1 Capture Assay

Evaluation of Rapid Blood Sample Collection in the Detection of Circulating Filarial Antigens for Epidemiological Survey by rWbSXP-1 Capture Assay

How can monoclonal antibodies be harnessed against neglected tropical diseases and other infectious diseases?

Electrochemical Biosensing for the Diagnosis of Viral Infections and Tropical Diseases

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