Antigen challenge increases adherence of circulating neutrophils in horses with chronic obstructive pulmonary disease

Marr, K.A.; Lees, P.; Cunningham, Fiona

doi:10.2746/042516402776181231

Cited by 16 publications

(9 citation statements)

References 27 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In asthmatic patients, systemic oxidative stress is explained as a result of increased superoxide generation by peripheral neutrophils . In RAO in horses, neutrophils circulating in the blood have also been shown to produce increased amounts of superoxide anions during exposure to organic dust . Thus, it is possible that a systemic imbalance in oxidant‐antioxidant status in RAO‐affected horses may be a consequence of the disease and activation of peripheral neutrophils.…”

Section: Discussionmentioning

confidence: 99%

Oxidant‐Antioxidant Status in the Blood of Horses with Symptomatic Recurrent Airway Obstruction (RAO)

Niedźwiedź

Jaworski

2014

Veterinary Internal Medicne

View full text Add to dashboard Cite

BackgroundSystemic oxidative stress in horses with recurrent airway obstruction (RAO) is poorly characterized.ObjectivesThe goal of this study was to investigate whether equine RAO is associated with systemic disturbances in the oxidant‐antioxidant equilibrium.AnimalsSeven healthy horses and 7 horses with symptomatic RAO.MethodsA prospective study. Healthy and RAO‐affected horses were exposed to a 48‐hour challenge with moldy hay and straw to induce clinical exacerbation of RAO. Venous blood was collected and the activities of the superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in equine erythrocyte lysates were measured. The concentration of thiobarbituric acid‐reactive substances (TBARSs) was assessed both in erythrocyte lysates and in plasma.ResultsA significant increase in the activities of GPx and SOD was detected in RAO‐affected horses compared with the control animals. There was no significant difference between groups in terms of the erythrocyte lysate activities of CAT, GR, or TBARs or the plasma concentration of TBARs.Conclusion and Clinical ImportanceOur results support the hypothesis that RAO in horses is associated with systemic oxidative stress. Future studies are needed to assess whether horses suffering from RAO can benefit from antioxidant supplementation.

show abstract

Section: Discussionmentioning

confidence: 99%

Oxidant‐Antioxidant Status in the Blood of Horses with Symptomatic Recurrent Airway Obstruction (RAO)

Niedźwiedź

Jaworski

2014

Veterinary Internal Medicne

View full text Add to dashboard Cite

show abstract

“…Despite the priming and activation of circulating neutrophils and their extensive and rapid (4–5 h) recruitment into the airway lumen following environmental challenge of RAO horses , there is relatively little evidence for their involvement in the induction of structural lung damage. Both the gene expression and protein secretion of Clara cell secretory protein, a recognised inhibitor of inflammation in the lung, are depleted in the airways of symptomatic RAO horses compared with controls , a finding that may reflect the entry of Clara cell secretory protein into luminal neutrophils, potentially inhibiting oxidative burst and enhancing phagocytosis .…”

Section: Pathogenesismentioning

confidence: 99%

Recurrent airway obstruction: A review

Pirie

2014

Equine Veterinary Journal

107

115

View full text Add to dashboard Cite

Recurrent airway obstruction is a widely recognised airway disorder, characterised by hypersensitivity-mediated neutrophilic airway inflammation and lower airway obstruction in a subpopulation of horses when exposed to suboptimal environments high in airborne organic dust. Over the past decade, numerous studies have further advanced our understanding of different aspects of the disease. These include clarification of the important inhaled airborne agents responsible for disease induction, improving our understanding of the underlying genetic basis of disease susceptibility and unveiling the fundamental immunological mechanisms leading to establishment of the classic disease phenotype. This review, as well as giving a clinical overview of recurrent airway obstruction, summarises much of the work in these areas that have culminated in a more thorough understanding of this debilitating disease.

show abstract

“…There is a modest but significant increase in peripheral blood neutrophil 7–24 h following the initiation of stable dust exposure 76,125,126 . Blood neutrophils also have an increased active respiratory burst and delayed spontaneous apoptosis 24 h after challenge 145–147 . Increased CD18‐dependant adherence is present, even during clinical remission, suggesting a persistent state of neutrophil activation 145 .…”

Section: Systemic Inflammationmentioning

confidence: 99%

Heaves, an asthma‐like disease of horses

Leclère¹,

Lavoie-Lamoureux²,

Lavoie³

2011

Respirology

176

233

View full text Add to dashboard Cite

Animal models have been developed to investigate specific components of asthmatic airway inflammation, hyper-responsiveness or remodelling. However, all of these aspects are rarely observed in the same animal. Heaves is a naturally occurring disease of horses that combines these features. It is characterized by stable dust-induced inflammation, bronchospasm and remodelling. The evaluation of horses during well-controlled natural antigen exposure and avoidance in experimental settings allows the study of disease mechanisms in the asymptomatic and symptomatic stages, an approach rarely feasible in humans. Also, the disease can be followed over several years to observe the cumulative effect of repeated episodes of clinical exacerbation or to evaluate long-term treatment, contrasting most murine asthma models. This model has shown complex gene and environment interactions, the involvement of both innate and adaptive responses to inflammation, and the contribution of bronchospasm and tissue remodelling to airway obstruction, all occurring in a natural setting. Similarities with the human asthmatic airways are well described and the model is currently being used to evaluate airway remodelling and its reversibility in ways that are not possible in people for ethical reasons. Tools including antibodies, recombinant proteins or gene arrays, as well as methods for sampling tissues and assessing lung function in the horse are constantly evolving to facilitate the study of this animal model. Research perspectives that can be relevant to asthma include the role of neutrophils in airway inflammation and their response to corticosteroids, systemic response to pulmonary inflammation, and maintaining athletic capacities with early intervention.

show abstract

Antigen challenge increases adherence of circulating neutrophils in horses with chronic obstructive pulmonary disease

Cited by 16 publications

References 27 publications

Oxidant‐Antioxidant Status in the Blood of Horses with Symptomatic Recurrent Airway Obstruction (RAO)

Oxidant‐Antioxidant Status in the Blood of Horses with Symptomatic Recurrent Airway Obstruction (RAO)

Recurrent airway obstruction: A review

Heaves, an asthma‐like disease of horses

Contact Info

Product

Resources

About