Antifungal Susceptibility Pattern among Candida species: An Evaluation of Disc Diffusion and Micro Broth Dilution Method

Jayachandran, Abirami Lakshmy; Katragadda, Radhika; Ravinder, Thyagarajan; Leela, K.V.; Manorajan, Lavanya; Hemalatha, S.; Kaliappan, Shanmugam

doi:10.5799/jmid.458457

Cited by 6 publications

(3 citation statements)

References 9 publications

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“…A good agreement between DD method and BMD was reported in India which is similar to that found in this study. 27 Limitations: This study was done only in one hospital of Peshawar. It might increase our knowledge about the causative species and susceptibility pattern of azoles antifungal drugs if samples are collected from different hospitals.…”

Section: Discussionmentioning

confidence: 99%

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Azoles resistance of candida species causing vulvo-vaginitis in reproductive age women at a tertiary care setting

et al. 2022

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Objectives: To study prevalence and resistance pattern to azoles of candida species causing vulvovaginitis in reproductive age women. Methods: Samples were collected from Hayatabad Medical Complex from November 2018 to May 2019. This cross-sectional study was conducted at the department of Microbiology, Institute of Pathology and Diagnostic Medicine, Khyber Medical University Peshawar, Pakistan. A total of 369 high vaginal swabs were collected. Candida was isolated by vaginal swabs inoculation on Sabouraud’s dextrose agar (SDA). Colonies on SDA were inoculated on Candida CHROM agar to identify candida species. Wet film microscopy and Gram staining were performed. Biochemical identification was done with 20C AUX. Antifungal susceptibility testing was done by disc diffusion and broth micro-dilution methods to find the resistance pattern of azole drugs. Fluconazole, Clotrimazole, Miconazole, Voriconazole and Itraconazole were the azoles drugs used. Results: Among 43%(n=158) positive candida cases, 44%(n=85) were non pregnant women while 41%(n=73) were pregnant. The Candida species distribution of 158 isolates was as follow; Candida albicans (C. albicans) 46.2%(n=73), Candida krusei (C. krusei) 29.1%(n=46), Candida parapsilosis (C. parapsilosis) 19%(n=30) and Candida glabrata (C. glabrata) 5.7%(n=9). Overall Candida isolates were highly resistant 72%(n=113) to Fluconazole while least resistant 21.5 % (n=34) to Itraconazole. Conclusion: C. albicans is the most prevalent specie involved in Vulvovaginal candidiasis. Candida species were found to be least resistant to Itraconazole followed by Voriconazole, Miconazole, Clotrimazole and Fluconazole. doi: https://doi.org/10.12669/pjms.38.8.5984 How to cite this:Zaman R, Ullah I, Adeeb H, Arif A. Azoles resistance of candida species causing vulvo-vaginitis in reproductive age women at a tertiary care setting. Pak J Med Sci. 2022;38(8):---------. doi: https://doi.org/10.12669/pjms.38.8.5984 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Section: Discussionmentioning

confidence: 99%

“…A good agreement between DD method and BMD was reported in India which is similar to that found in this study. 27 …”

Section: Discussionmentioning

confidence: 99%

Azoles resistance of candida species causing vulvo-vaginitis in reproductive age women at a tertiary care setting

et al. 2022

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“…More recently, the overall resistance of Candida spp . was estimated as 21.7% and 17.3% against fluconazole and itraconazole, respectively 34 …”

Section: Discussionmentioning

confidence: 99%

In vitro antifungal susceptibility testing of fungi in patients with onychomycosis

El‐Hamd

Elhameed

Shalaby

et al. 2020

Dermatologic Therapy

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Onychomycosis is the most common nail disorder. To examine in vitro antifungal susceptibility of fungi among onychomycosis patients. The study included 68 patients with onychomycosis. Nail specimens were cultured on Sabouraud dextrose agar and Dermasel agar base‐media. Isolated fungi were subjected to antifungal susceptibility tests against terbinafine, itraconazole, fluconazole, and griseofulvin. Candida species (Candida spp.) were detected in 32.4% of the cases of candidal onychomycosis (n = 37), 23.5% of the cases of distal and lateral subungual onychomycosis (n = 17), and 21.4% of the cases of total dystrophic onychomycosis (n = 14). Candida spp. were sensitive to fluconazole in 73.5%, itraconazole in 58.8%, and terbinafine in 5.9% of the cases. Aspergillus spp. were sensitive to itraconazole in all cases, and terbinafine in 87.5% of cases. Penicillium spp. were sensitive to itraconazole and terbinafine in 88.9% and 77.8% of cases, respectively. Trichophyton spp. were sensitive to terbinafine and resistant to itraconazole. Microsporum spp. were sensitive to itraconazole and resistance to terbinafine. All isolated fungi were resistant to griseofulvin. An increasing proportion of Candida spp. was observed among patients with different clinical varieties of onychomycosis. Candida spp. were highly sensitive to fluconazole and a lesser extent to itraconazole.

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Inhibitory effects of cold atmospheric plasma on the growth, virulence factors and HSP90 gene expression in Candida albicans

Ebrahimi-Shaghaghi

Noormohammadi

Atyabi

et al. 2021

Archives of Biochemistry and Biophysics

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Antifungal Susceptibility Pattern among Candida species: An Evaluation of Disc Diffusion and Micro Broth Dilution Method

Cited by 6 publications

References 9 publications

Azoles resistance of candida species causing vulvo-vaginitis in reproductive age women at a tertiary care setting

Azoles resistance of candida species causing vulvo-vaginitis in reproductive age women at a tertiary care setting

In vitro antifungal susceptibility testing of fungi in patients with onychomycosis

Inhibitory effects of cold atmospheric plasma on the growth, virulence factors and HSP90 gene expression in Candida albicans

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