Inhibitory effects of cold atmospheric plasma on the growth, virulence factors and HSP90 gene expression in Candida albicans

Ebrahimi-Shaghaghi, Fatemeh; Noormohammadi, Zahra; Atyabi, Seyed-Mohammad; Razzaghi-Abyaneh, Mehdi

doi:10.1016/j.abb.2021.108772

Cited by 19 publications

(23 citation statements)

References 39 publications

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“…RONS cause photodesorption or etching of the cell wall, peroxidation of lipids and ergosterol, the latter being a critical sterol of the cell membrane [57]. This leads to an increase in membrane permeability favoring leakage of cytoplasm and intracellular components, and ultimately to lethal cellular damage [40,53,58,59]. Our SEM images highlighted the rupture of the cell membrane and the leakage of intracellular components after a 120-min O 2 NTP, which is in accordance with the previous hypotheses.…”

Section: Discussionsupporting

confidence: 91%

Non-Thermal O2 Plasma Efficacy on C. albicans and Its Effect on Denture Base Resin Color

et al. 2021

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Denture stomatitis is a disease involving C. albicans, which can affect elderly and immuno-compromised people. To avoid any recurrence of this pathology, it is necessary to treat patients regularly and disinfect dentures. However, the denture cleansers’ efficacy is not optimal and often leads to adverse color effects on the denture base resins. The aim of this study was to investigate the efficacy of a low-pressure non-thermal O2 plasma (NTP) treatment on C. albicans seeded on ProBase®Hot resin (Ivoclar Vivadent). The viability reduction of C. albicans was assessed by colony forming units (CFU) analysis and by scanning electron microscopy (SEM). The effect of repeated treatments on the resin color was evaluated by spectrophotometry. The resin samples were placed in a sealed bag in which O2 plasma was generated in low-pressure conditions. The results showed that a 120-min O2 NTP treatment led to a 6-log reduction of C. albicans viability (p < 0.05) and to yeasts’ major alterations observed by SEM. Furthermore, significant slight color changes of the resin (∆E00 = 1.33) were noted only after six plasma treatments (p < 0.05). However, the denture aesthetic was preserved, as the color changes were not perceptible and remained below the acceptability threshold (∆E00 < 4).

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Section: Discussionsupporting

confidence: 91%

Non-Thermal O2 Plasma Efficacy on C. albicans and Its Effect on Denture Base Resin Color

et al. 2021

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“…The results show that there was no synergy, with no significant difference ( p > 0.05) between the isolated treatments and the associations. Other authors verified the increased susceptibility of C. albicans cells to amphotericin B previously exposed to cold helium and oxygen plasma for 180 s. [ 27 ]. In this case, however, the CAP exposure parameters were different from our study, in addition to the concentration of amphotericin B (20 µg/mL) and the conditions of the fungal cells, which were in the planktonic form.…”

Section: Discussionmentioning

confidence: 77%

“…Candida albicans are fungal pathogens often associated with cases of mortality from systemic infection [ 27 , 28 ]. Biofilm formation, one of the virulence factors, stands out for its appearance on different surfaces [ 29 ] and for its greater infectious capacity [ 30 ] Biofilm infections of C. albicans can result in increased length of hospital stay, increased cost of antifungal therapy and risk of death [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals on Candida albicans Biofilms

et al. 2021

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The increasing incidence of antifungal resistance represents a great challenge in the medical area and, for this reason, new therapeutic alternatives for the treatment of fungal infections are urgently required. Cold atmospheric plasma (CAP) has been proposed as a promising alternative technique for the treatment of superficial candidiasis, with inhibitory effect both in vitro and in vivo. However, little is known on the association of CAP with conventional antifungals. The aim of this study was to evaluate the effects of the association between CAP and conventional polyene antifungals on Candida albicans biofilms. C. albicans SC 5314 and a clinical isolate were used to grow 24 or 48 h biofilms, under standardized conditions. After that, the biofilms were exposed to nystatin, amphotericin B and CAP, separately or in combination. Different concentrations of the antifungals and sequences of treatment were evaluated to establish the most effective protocol. Biofilms viability after the treatments was compared to negative control. Data were compared by One-way ANOVA and post hoc Tukey (5%). The results demonstrate that 5 min exposure to CAP showed more effective antifungal effect on biofilms when compared to nystatin and amphotericin B. Additionally, it was detected that CAP showed similar (but smaller in magnitude) effects when applied in association with nystatin and amphotericin B at 40 µg/mL and 60 µg/mL. Therefore, it can be concluded that the application of CAP alone was more effective against C. albicans biofilms than in combination with conventional polyene antifungal agents.

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“…Apigenin, rutin, and palmitic acid downregulated the expression of ERG11 [ 56 , 104 ]. Other studies targeted ergosterol biosynthesis by applying tormentic acid [ 105 ], benzodioxane derivatives [ 106 ], honokiol and magnolol [ 107 ], and cold atmospheric plasma [ 108 ].…”

Section: Recent Update On Antifungal Targets and Strategiesmentioning

confidence: 99%

Emerging Antifungal Targets and Strategies

Ivanov

Ćirić

Stojković

2022

IJMS

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Despite abundant research in the field of antifungal drug discovery, fungal infections remain a significant healthcare burden. There is an emerging need for the development of novel antifungals since those currently available are limited and do not completely provide safe and secure protection. Since the current knowledge regarding the physiology of fungal cells and the infection mechanisms is greater than ever, we have the opportunity to use this for the development of novel generations of antifungals. In this review, we selected and summarized recent studies describing agents employing different antifungal mechanisms. These mechanisms include interference with fungal resistance, including impact on the efflux pumps and heat shock protein 90. Additionally, interference with virulence factors, such as biofilms and hyphae; the impact on fungal enzymes, metabolism, mitochondria, and cell wall; and antifungal vaccines are explored. The agents investigated belong to different classes of natural or synthetic molecules with significant attention given also to plant extracts. The efficacy of these antifungals has been studied mainly in vitro with some in vivo, and clinical studies are needed. Nevertheless, there is a large quantity of products employing novel antifungal mechanisms that can be further explored for the development of new generation of antifungals.

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Inhibitory effects of cold atmospheric plasma on the growth, virulence factors and HSP90 gene expression in Candida albicans

Cited by 19 publications

References 39 publications

Non-Thermal O2 Plasma Efficacy on C. albicans and Its Effect on Denture Base Resin Color

Non-Thermal O2 Plasma Efficacy on C. albicans and Its Effect on Denture Base Resin Color

Effect of Cold Atmospheric Plasma Jet Associated to Polyene Antifungals on Candida albicans Biofilms

Emerging Antifungal Targets and Strategies

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