Antifungal effects of iron sulfate on grapevine fungal pathogens

Fleurat‐Lessard, Pierrette; Dédaldéchamp, Fabienne; Thibault, Florence; Béré, Emile; Roblin, Gabriel

doi:10.1016/j.scienta.2011.07.004

Cited by 15 publications

(18 citation statements)

References 46 publications

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“…In the previous study, it was shown that the structure of the B. cinerea hyphae changed after treatment with antibiotics, eugenol, FeSO4 and tea tree oil (Šašek and Musilek. 1974;Wang et al 2010;Fleurat-Lessard et al 2011;Shao et al 2013). We found that TPPS also induces a structural change on the fungus.…”

Section: Discussionmentioning

confidence: 64%

“…In particular, the addition of an ammonium counterion to the sulfate moiety contributed to the inhibition of mycelium growth in the pathogenic fungus Eutypa lata, an ascomycete like B.cinerea. Therefore, taking these results from previous works into account (Fleurat-Lessard et al 2011;Guillaumot et al 2016;Issawi et al 2018b;, we decided to test TPPS, a molecule that presents four external sulfonate groups linked with a tetrapyrrole ring. Indeed, TPPS with an ammonium counterion could be as an excellent antifungal candidate.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Photodynamic inactivation of Botrytis cinerea for a safe pest management of grapevine

Ambrosini¹,

Issawi²,

Sol³

et al. 2020

Preprint

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Botrytis cinerea is a necrotic fungal plant pathogen responsible for the gray mold disease on more than 200 crops, including grapevine. Due to its genetic plasticity, this fungus presents a strong resistance to a large spectrum of fungicides. Thus, new fighting strategies against B. cinerea are urgently needed. In this context, antimicrobial photodynamic treatment (APDT) was considered. APDT involves the use of a photosensitizer that generates reactive oxygen species upon illumination. Tetra-4-sulfonatophenyl porphyrin tetra-ammonium (TPPS) was tested on B. cinerea; upon light exposure. 1.5 µM TPPS was shown to completely inhibit mycelial growth. A concentration of 12.5 µM TPPS was tested on three genetic background clones from Chardonnay, Merlot and Sauvignon, grown in vitro for 2 months. Treated root apparatus of the three backgrounds was able to increase thiol production as a molecular protection against photoactivated TPPS, leading to a normal phenotype as compared with control plantlets. Finally, two-month-old grapevine leaves were infected with four-day-old mycelium of B. cinerea pre-incubated or not with TPPS. The pre-treated mycelium was unable to infect the isolated leaves of any of the three grapevine varieties after 72 h growth when subjected to a 16 h photoperiod, contrary to untreated mycelium. These results on fungus, plantlets and Botrytis-grapevine leaves, in contact with a very low concentration of TPPS, suggest a strong potential of photo-treatment against Botrytis mycelium for future agricultural practices in vineyard or other cultures.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Photodynamic inactivation of Botrytis cinerea for a safe pest management of grapevine

Ambrosini¹,

Issawi²,

Sol³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Assuming the desired in vitro fungicidal effect of ozonated water is a fact, a successful control tool should target the pathogen at the right stage in its infection cycle. For instance, 5 mM in vitro iron sulfate inhibit P. aleophilum growth by 40% and appear to be a promising control agent when applied to soil supplements because this compound is absorbed by the roots and redistributed throughout the plant via xylem translocation (Fleurat-Lessard et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

In vitro and in planta fungicide properties of ozonated water against the esca-associated fungus Phaeoacremonium aleophilum

Pierron

Pagès

Couderc

et al. 2015

Scientia Horticulturae

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“…In a similar way, Fleurat-Lessard et al 53 discovered a strategy whereby they investigated the potential of FeSO 4 and found that the sulfate anion determines the inhibition of mycelium growth in pathogenic fungi that is similar to B. cinerea at high concentrations (range of 0.5-20 mM). They also tested different iron salts and found that those with bromide, chloride and sulfate anions showed the best antifungal activity.…”

Section: Rootsmentioning

confidence: 99%

“…Therefore, taking these results from previous works into account 27,28,30,53 , we decided to test TPPS, a molecule that presents four external sulfonate groups linked by a tetrapyrrole ring. Indeed, TPPS with an ammonium counterion could be an excellent antifungal candidate.…”

Section: Rootsmentioning

confidence: 99%

Photodynamic inactivation of Botrytis cinerea by an anionic porphyrin: an alternative pest management of grapevine

Ambrosini

Issawi

Sol

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Botrytis cinerea is a necrotic plant fungus that causes gray mold disease in over 200 crops, including grapevine. Due to its genetic plasticity, this fungus presents strong resistance to many fungicides. Thus, new strategies against B. cinerea are urgently needed. In this context, antimicrobial photodynamic treatment (APDT) was considered. APDT involves the use of a photosensitizer that generates reactive oxygen species upon illumination with white light. Tetra-4-sulfonatophenyl porphyrin tetra-ammonium (TPPS) was tested on B. cinerea using light. 1.5 µM TPPS completely inhibited mycelial growth. TPPS (12.5 µM) was tested on three grapevine clones from Chardonnay, Merlot and Sauvignon, grown in vitro for 2 months. Treated root apparatus of the three backgrounds increased thiol production as a molecular protection against photoactivated TPPS, leading to a normal phenotype as compared with control plantlets. Finally, 2-month-old grapevine leaves were infected with 4-day-old mycelium of B. cinerea pre-incubated or not with TPPS. The pre-treated mycelium was unable to infect the detached leaves of any of the three grapevine varieties after 72 h growth when subjected to a 16 h photoperiod, contrary to untreated mycelium. These results suggest a strong potential of photo-treatment against B. cinerea mycelium for future agricultural practices in vineyard or other cultures.

show abstract

Antifungal effects of iron sulfate on grapevine fungal pathogens

Cited by 15 publications

References 46 publications

Photodynamic inactivation of Botrytis cinerea for a safe pest management of grapevine

Photodynamic inactivation of Botrytis cinerea for a safe pest management of grapevine

In vitro and in planta fungicide properties of ozonated water against the esca-associated fungus Phaeoacremonium aleophilum

Photodynamic inactivation of Botrytis cinerea by an anionic porphyrin: an alternative pest management of grapevine

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