Antifungal Effects of Bacilysin and Fengymycin from Bacillus subtilis F‐29‐3 A Comparison with Activities of Other Bacillus Antibiotics

Abstract

Of the two antifungal antibiotics produced by Badllus subtilis F-29-3, the dipeptide compound bacilysin inhibits yeasts (and bacteria), whereas the formerly unknown fengymycin, a complex of closely related lipopeptide components, shows antibiotic activity against filamentous fungi.Bacilysin production, formerly known for a few strains only, could be demonstrated for all 12 wild-type cultures of Badllus subtilis tested during this study. The antibiotic also occurs in some strains of three other Badllus species considered as closely realted to B. subtilis.Members of the lipopeptide class of antifungal Badllus metabolites were formed by 8 of 12 Badllus subtilis-isolztes and several other Badllus strains.The antibiotics of F-29-3 were compared with antifungal metabolites of other Badllus isolates using TLC, agar-diffusion techniques and tests demonstrating the capacity of six lipopeptide and peptide preparations to protect rice seedlings from phytomycosis due to Rbizoctonia solani.Fengymycin proved to be different from the other compounds tested. It was less toxic to the test plants and protected them better from Rhizoctonia disease than the other antibiotics of the study did.

The production of antifungal volatiles by Bacillus subtilis

“…All the work presented so far points to the principal mode of action of this antagonist as the production of antibiotics (Loeffler et al 1986;McKeen et al 1986). Although these antibiotics typically have a broad spectrum of activity against many genera of fungi (Utkhede and Scholberg 1986), little is known about other possible antagonistic modes of action which are available to B .…”

Isolation and Selection of Antagonistic Microbes for Biological Control of Zoysiagrass Large Patch Disease

“…물의 경우 종에 따라 차이가 있고 생성되는 항균물질(iturin, surfactin 등)도 차이가 나타나는 것으로 알려져 구체적인 항균 기작에 관한 자세한 연구가 필요할 것으로 사료된다 (Elkahoui et al, 2012;Yu et al, 2002). et al, 1964;Gregory et al, 1952;Leoffler et al, 1986). .…”

Characterization of Bacillus licheniformis KJ-9 Isolated from Soil

“…여러 가지 식물병원성 진균에 대한 길항균주를 이용한 생태 학적 생물방제방법에는 첫째는 진균 외막가수분해효소인 chitinase, β-1,3-glucanase와 같은 효소에 의해 식물병원균의 세 포벽을 분해시키는 용균작용 [14], 둘째는 Streptomyces griseus [4], Streptomyces blastmyces [24], Penicillium nigricans [3], Bacillus subtilis [17] 및 Pseudomonas sp. [2] 등이 생산하는 항진 균성 항생물질에 의해 직접 식물병원균의 생육을 저해시키는 항생작용(antibiosis), 셋째는 식물성장 촉진세균(plant growthpromoting rhizobacteria, PGPR) 즉, 대부분 근권 Pesudomonas sp.이 분비하는 철(Fe 3+ )성분 결합물질인 siderophore에 의해 식물병원균의 생육을 저해하는 경쟁적 길항작용(compective antagonism)을 이용한 방제방법 등이 있다 [19].…”